FastQCFastQC Report
Sat 16 Sept 2023
SRR15906260_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15906260_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27230580
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA6069932.2290858292405082No Hit
ACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG5137121.886526104107955No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2257580.8290605635282099Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAAT2073230.7613609405308297No Hit
TTGGGTTCGTGGCGGCTAGCGTTTCTGTAGGCACCATCAATAGATCGGAA1962100.7205502049534016No Hit
TTGGGTTCGTGGCGGCTAGCCTGTAGGCACCATCAATAGATCGGAAGAGC1418400.5208849756413562No Hit
ACGGTGGCCATGGAAGTCGGTATCCGCTCTGTAGGCACCATCAATAGATC1388630.5099524137936099No Hit
TTGGGTTCGTGGCGGCTAGCGTTTACAGGGTTGTTCTGTAGGCACCATCA1179320.43308662540423304No Hit
TTAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAA1155060.42417752394550534No Hit
TCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAG1153950.4237698939941786No Hit
ATTCAGTAGAGCAGTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1016510.37329722686773475No Hit
ACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG926510.34024614973313094No Hit
TTGCGGTCCGGATCGTATGGGTTATCAATTCTGTAGGCACCATCAATAGA904260.3320751889970761No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA794040.29159863653289797No Hit
TAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGA783030.28755538809676473No Hit
TCTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG762370.2799683297234212No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATA734770.2698326660688094No Hit
TGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGA718840.2639826254159845No Hit
GTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC718390.2638173700303115No Hit
ATGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA676630.24848167023985535No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGA675950.24823195099039386No Hit
ATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC673960.24750115495152875No Hit
TCGAGTACAGACCGCGAAAGCGTGGCCTATCGCTGTAGGCACCATCAATA557810.2048469037383706No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC546690.20076325954129512No Hit
TTTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGG503200.18479224460147378No Hit
TTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG490170.18000718310076394No Hit
AGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAATA487570.17905237420576425No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAG462080.16969157469286367No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC434640.15961466850871336Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATA427170.15687142910654125No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCCTGTAGGCACCATCAATAG422130.15502056878700343No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAATA414260.15213043570867754No Hit
TGCGGTCCGGATCGTATGGGTTATCAATTCTGTAGGCACCATCAATAGAT409680.15044850311671656No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGAT403480.14817165113633277No Hit
TTAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGGCTGTAGGCACC398570.14636853126154492No Hit
TAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGG385450.14155041868370047No Hit
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAAT380440.1397105753898742No Hit
ATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGGAAGAGC378850.13912667302716286No Hit
ATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCT377470.13861988984443227TruSeq Adapter, Index 4 (100% over 43bp)
ACGTGAAATCGCTGGAGTGGAACCGGAGACAGCTGTAGGCACCATCAATA376950.13842892806543233No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAG375650.13795152361793248No Hit
TCCGCTTGTCGAAAGGGAATAGGGTTAATATTCCCTAACTGAGATCTGTA373540.1371766594762212No Hit
ATTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGG371810.13654134432685605No Hit
TCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAGATC371730.1365119655916253No Hit
CTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA357350.13123113793389637No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAATA350650.1287706688583203No Hit
TGTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGG329030.1208310656622077No Hit
ATCATGGAATAATGAAACTGTAGGCACCATCAATAGATCGGAAGAGCACA321070.11790788150674719No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAAT316330.11616719144432472No Hit
ACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAATAGATCG315640.11591379985295942No Hit
ATAGGTCCGGAGTCGGTGGGTTATCTGACTGTAGGCACCATCAATAGATC309250.11356717337640257No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA307960.11309344127080657No Hit
TCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAATAGAT304310.1117530364759032No Hit
AGAACGTGAAATCGCTGGAGTGGAACCGGAGACAGCTGTAGGCACCATCA297270.10916770777559641No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG295080.10836346489865438No Hit
ACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT288580.10597644266115522No Hit
TTTGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCAT287130.1054439530850977No Hit
ATCGCTGGAGTGGAACCGGAGACAGCTGTAGGCACCATCAATAGATCGGA285560.10486739540619408No Hit
TCGAAATGTGGCGTCCTTGGAGTCGGGTTGCTCTGTAGGCACCATCAATA279410.10260890513532947No Hit
ATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATAGATCGGAAGAGCA277080.10175324947173361No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)