FastQCFastQC Report
Sat 16 Sept 2023
SRR15906261_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15906261_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12883026
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG2865562.2242910943438288No Hit
TTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA2626532.038752386279435No Hit
ACGGTGGCCATGGAAGTCGGTATCCGCTCTGTAGGCACCATCAATAGATC1156870.8979800242582759No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1098710.8528353509493811Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGGCTGTAGGCACC760750.590505677780981No Hit
TAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAAT698940.5425278191629823No Hit
ACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG588950.45715191446481596No Hit
TAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGA508620.3947985512099409No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATA447140.34707684359249136No Hit
TTAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAA442190.343234578584255No Hit
TCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAG431770.335146416688129No Hit
TTGGGTTCGTGGCGGCTAGCGTTTCTGTAGGCACCATCAATAGATCGGAA429910.33370265650321596No Hit
TCGAGTACAGACCGCGAAAGCGTGGCCTATCGCTGTAGGCACCATCAATA408390.3169985064068022No Hit
TTGCGGTCCGGATCGTATGGGTTATCAATTCTGTAGGCACCATCAATAGA382540.2969333446971232No Hit
TTGGGTTCGTGGCGGCTAGCCTGTAGGCACCATCAATAGATCGGAAGAGC369610.2868968827665177No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA364530.28295370978836804No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC350310.2719159303101616No Hit
ATTCAGTAGAGCAGTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA334370.25954306076848715No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGA306780.23812728469227648No Hit
TCTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG300250.23305859974201712No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAATA281490.2184968034683777No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCCTGTAGGCACCATCAATAG278630.21627682813028554No Hit
TCTAAGTTAGAATCTCGCCTTGTCAAGGCGAAAATTCTGTAGGCACCATC258140.2003721796416463No Hit
TTTGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCAT257350.1997589696706348No Hit
TTAGGCAATAAAAATTTACCTCGGCAATTTATCGCTTCTGTAGGCACCAT245260.19037452846869982No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC241950.1878052563116771Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA239500.18590352918638836No Hit
TTTAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGGCTGTAGGCAC230910.1792358410205801No Hit
AGAACGTGAAATCGCTGGAGTGGAACCGGAGACAGCTGTAGGCACCATCA228470.17734187604682317No Hit
TGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGA223330.17335213015948273No Hit
ATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGGAAGAGC218430.16954867590890524No Hit
AGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAATA214090.16617990214410808No Hit
GTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC201000.15601924578899398No Hit
TAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGG200540.1556621868185316No Hit
TTTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGG199000.15446681548263583No Hit
ATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCT197880.15359745451107526TruSeq Adapter, Index 5 (100% over 43bp)
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATA192660.1495456114114805No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAAT187870.14582754082775273No Hit
TTGGGTTCGTGGCGGCTAGCGTTTACAGGGTTGTTCTGTAGGCACCATCA182040.14130220648471875No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAG181280.14071228296830263No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA179020.13895803672211793No Hit
ATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATAGATCGGAAGAGCA178350.13843797256948795No Hit
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAAT177510.13778595184081752No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGAT172630.13399802189330365No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAA167300.12986079512685916No Hit
TGCGGTCCGGATCGTATGGGTTATCAATTCTGTAGGCACCATCAATAGAT164600.12776501421327566No Hit
ACCTGCAGCTGGATCATCGCTGTAGGCACCATCAATAGATCGGAAGAGCA163880.12720613930298674No Hit
ATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC163530.12693446399937408No Hit
ATCATGGAATAATGAAACTGTAGGCACCATCAATAGATCGGAAGAGCACA161200.12512588269246683No Hit
ACGTGAAATCGCTGGAGTGGAACCGGAGACAGCTGTAGGCACCATCAATA157060.12191235195830544No Hit
TAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGGCTGTAGGCACCA154330.11979328459012657No Hit
ACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG153470.11912573955839256Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA145740.11312559642431833No Hit
TTTGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTCTGTAGGCACCATC145720.11311007212125475No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAG145220.11272196454466521No Hit
TCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAGATC142180.11036227047900081No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAATA140240.10885641308183343No Hit
ACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCA137500.10672958356212275No Hit
TTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG136460.10592231980281651No Hit
TTAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGCTGTAGGCACCA135130.10488995364908835No Hit
TGTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGG133430.10357038788868392No Hit
TACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCG132610.10293389146307708No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG130380.10120293167148774No Hit
TTAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGG129910.10083811054949357No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)