FastQCFastQC Report
Sat 16 Sept 2023
SRR15906272_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15906272_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32888254
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC10875723.306870592765429No Hit
CTAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCG7075382.151339502547019No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATA6799052.067318623846678No Hit
TAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGG6599052.006506639118027No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGAT6385941.9417084287904125No Hit
TAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGA6050261.8396415936218447No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGA5684941.7285624223164904No Hit
TGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGAA5551011.687839676742949No Hit
TTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA5549891.6874991296284687No Hit
TCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAG5039201.5322187672230945No Hit
ACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG5000201.5203604302010074No Hit
CTAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGG4992211.5179309914110977No Hit
GACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAAGA4791981.4570490729000085No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG4294351.3057397330974152No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAATAG3735251.1357398297884709No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAAT3598201.0940684172531627No Hit
ACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG3572211.0861658998376746No Hit
GTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA3275620.9959847670843214No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAATA3197150.972125184876035No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTCTGTAGGCACCATCAATAGATCG3194930.971450171845547No Hit
TGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGA2905140.883336646572968No Hit
CGACGTGGGATCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2806810.8534384342811266No Hit
TCGACGTGGGATCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2716990.8261277719394894No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2267920.6895835820290125Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATAG1785820.5429962928405989No Hit
AAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAA1719660.5228796882923612No Hit
AAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGGA1672990.5086892116559304No Hit
TAGGTGGGAGTCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1562460.47508146829564135No Hit
GACGTGGGATCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1539350.4680546434602457No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAAT1349310.41027109557108143No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATA1290790.392477508839478No Hit
GTGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGG1278310.38868284099241024No Hit
TACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGAT1236900.376091719554343No Hit
ACGCCGACGGCGTTGGCTGCTCGCTGTAGGCACCATCAATAGATCGGAAG1212760.36875171299759485No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTCTGTAGGCACCATCAATAGA1162030.35332675307117245No Hit
TACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATC1147590.3489361277737638No Hit
ACGCCGACGGCGTTGGCTGCTCCTGTAGGCACCATCAATAGATCGGAAGA1106790.336530482889119No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGAT1089920.3314009919772573No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAA1050790.3195031271650967No Hit
GTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATAGA1036140.315048649283723No Hit
ACTGCGATCTGTTGAGACTATCCTTTGACTGTAGGCACCATCAATAGATC951510.2893160579457943No Hit
TCGACTCTTTACTTATTCGGTTAAACTGTAGGCACCATCAATAGATCGGA918230.27919694368694675No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGTCTGTAGGCACCATCAA895620.2723221488133727No Hit
GACGACAGTGAAATACCACCACTCTGTAGGCACCATCAATAGATCGGAAG890330.2707136718172999No Hit
GCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGAA887920.2699808874013196No Hit
TGCAATTATTAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC861030.26180471605455247No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA842710.256234338253408No Hit
TACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGA828710.25197749932240243No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAA824350.2506517980553179No Hit
GTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAA807800.245619606319022No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGCTGTAGGCACCATCAAT807750.2456044033228398No Hit
TACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCG775080.23567076561741468No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGATC772980.23503223977776383No Hit
CGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAGA765860.23286733312142383No Hit
TGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAAG759430.23091222781239773No Hit
TGCAGCGCACCACGGCCCTGTGCGTGCTGTAGGCACCATCAATAGATCGG758540.2306416144803552No Hit
ACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAAGAG748230.22750675666759324No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCAGCTGTAGGCACCATCAAT740960.2252962410227068No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAATA738540.22456041600749008No Hit
TGCTCGTTCTACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG707220.2150372591989833Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTCTTTACTTATTCGGTTAAACTGTAGGCACCATCAATAGATCGGAA701080.21317033126781373No Hit
CCGACGTGGGATCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC681340.20716818837509587No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATCTGTAGGCACCATCAATAGAT674540.20510058089432173No Hit
TTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATCGGAAGAGCACAC669490.2035650782799233No Hit
TCGACTCTTTACTTATTCGGTTAACTGTAGGCACCATCAATAGATCGGAA641420.19503011622325708No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGA608550.18503566653310327No Hit
TCGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC607650.18476201260182434No Hit
TTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA603500.18350016391870483No Hit
TGCTCGTTCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT597180.18157850520127947Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGGAAGAGC589710.17930717757166434No Hit
AGTGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCG570580.17349051123236886No Hit
GTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATCGGAAGAGCACA542320.16489777779021045No Hit
TCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAG525400.15975308388216655No Hit
TGTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGG515530.15675201243580764No Hit
CGACTCTTTACTTATTCGGTTAACTGTAGGCACCATCAATAGATCGGAAG510650.15526820000842853No Hit
TGGGTTCGTGGCGGCTAGCCTGTAGGCACCATCAATAGATCGGAAGAGCA504360.15335566308871246No Hit
ACGCCGACGGCGTTGGCTGCTCGTCTGTAGGCACCATCAATAGATCGGAA504230.15331613529863886No Hit
AATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAG483220.14692783630289402No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAATA471600.1433946599901594No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAG470310.14300242268865962No Hit
AGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAGAGCAC456620.13883984233398344No Hit
TAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAG452130.13747461327682522TruSeq Adapter, Index 13 (100% over 35bp)
TTCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT440440.13392015276943556Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGCAATTATTAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT439530.1336434582389202No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCCTGTAGGCACCATCAATAGATCGG427470.12997649555978255No Hit
ACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCA418190.12715481946837312No Hit
TTGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATC409300.12445172674718458No Hit
GGGTAAACGGCGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC401230.1219979631633835No Hit
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAA392470.11933439823226857No Hit
TGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGATCGGAA391350.11899385111778812No Hit
TTTGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAAT375050.11403767436240306No Hit
GAGTGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATC372350.11321671256856627No Hit
TACGCCGACGGCGTTGGCTGCTCGTTCTCTGTAGGCACCATCAATAGATC367960.11188188950377238No Hit
GGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGAAG362920.11034942748861037No Hit
CGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATCG361140.10980820082452536No Hit
CTTGGTCAAGGTGTTGTACTGTAGGCACCATCAATAGATCGGAAGAGCAC351420.10685273836671293No Hit
GTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCAC350060.10643921687055811No Hit
ATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT348620.10600137058051182No Hit
CCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGAT337870.10273272640134681No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC332540.10111208700832827Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)