FastQCFastQC Report
Sat 16 Sept 2023
SRR15906273_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15906273_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29367720
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGAT25864278.807040519318491No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC10363043.5287179256680465No Hit
TACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGA5843191.989664161875692No Hit
TTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA5445901.854382975593611No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAAT5195861.769241875092789No Hit
CTAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCG4880131.6617326779198385No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTCTGTAGGCACCATCAATAGATCG4534371.543997967836795No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAA3675461.25153059209227No Hit
GTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA3584071.2204113904654499No Hit
TCGACGTGGGATCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2983171.0157989792874627No Hit
TTGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATC2817550.9594037262681611No Hit
TGTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGG2498330.8507061494729586No Hit
TAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGG2369600.8068723074178044No Hit
TACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGAT2282850.7773330718217145No Hit
CTAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGG1878410.6396172396086588No Hit
TCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAG1762530.6001589500308502No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATA1744340.5939650745784828No Hit
TGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGAA1717110.5846929894455545No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGA1632040.5557258105157636No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGCTGTAGGCACCATCAAT1382560.47077539557037457No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAATAG1260700.4292808566684782No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAATA1256550.42786774049875176No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAAT1236750.4211256440745144No Hit
TGCAGCGCACCACGGCCCTGTGCGTGCTGTAGGCACCATCAATAGATCGG1232850.419797655384892No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAAT1198800.40820329259472643No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG1155810.39356477111604166No Hit
ACGCCGACGGCGTTGGCTGCTCGCTGTAGGCACCATCAATAGATCGGAAG1147070.39058871441160564No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATA1142890.3891653829442667No Hit
TAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGA1141850.38881125262703403No Hit
TCGACTCTTTACTTATTCGGTTAAACTGTAGGCACCATCAATAGATCGGA1117390.38048237997365814No Hit
ACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG1114660.3795527878909224No Hit
TACGCCGACGGCGTTGGCTGCTCGTTCTCTGTAGGCACCATCAATAGATC1109530.37780597199918825No Hit
TCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAG1090310.37126137132879233No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAATA1058220.36033440798264216No Hit
TCGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGAT1046650.3563947082034288No Hit
ATACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAA1021580.3478581244985991No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAA1015820.34589678735700286No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA1012140.3446437108498719No Hit
ACGCCGACGGCGTTGGCTGCTCCTGTAGGCACCATCAATAGATCGGAAGA999450.3403226399597926No Hit
TTTGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCAT955680.32541852074318334No Hit
CCGACGTGGGATCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC950310.3235899824705493No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCAGCTGTAGGCACCATCAAT948100.32283745554642984No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCA893230.30415367621320283No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCCTGTAGGCACCATCAATAGATCGG885850.3016407130005326No Hit
TGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGA853270.2905468997933786No Hit
TGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCA841700.28660720001416523No Hit
TAGGTGGGAGTCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC714610.24333179422849305No Hit
CTTGGTCAAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT708520.2412580888131595Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAG696570.23718899526418802No Hit
TTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATA672910.22913253054714497No Hit
TACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATC669450.22795436622250553No Hit
TCGACTCTTTACTTATTCGGTTAAAACTGTAGGCACCATCAATAGATCGG665060.22645952767187918No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTCTGTAGGCACCATCA658900.2243619865621165No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTGTAGGCACCATCAATAGATCGGA657890.2240180715424963No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTCTGTAGGCACCATCAATAGA629850.2144701733740311No Hit
TTGGGTTCGTGGCGGCTAGCGTTTAGCTGTAGGCACCATCAATAGATCGG619200.21084374272160047No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCCTGTAGGCACCATCAATAG617490.21026147075768903No Hit
GTGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGG604770.20593018456999726No Hit
TACTGCGATCTGTTGAGACTATCCTTTGATTGAGCTGTAGGCACCATCAA598340.20374070578172226No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTCTGTAGGCACCATC588920.20053310233140334No Hit
GACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAAGA568750.19366501723661217No Hit
ACGCCGACGGCGTTGGCTGCTCGTCTGTAGGCACCATCAATAGATCGGAA561100.1910601163454296No Hit
CGACGTGGGATCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG548430.18674585565375862No Hit
TTGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTGGCTGTAGGCACCAT547630.1864734477174258No Hit
AAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGGA545330.18569027490046897No Hit
GTGCAGCGCACCACGGCCCTGTGCGTGCTGTAGGCACCATCAATAGATCG482800.16439818957685512No Hit
ACTGCGATCTGTTGAGACTATCCTTTGACTGTAGGCACCATCAATAGATC470990.16037676741674192No Hit
TCGACTCTTTACTTATTCGGTTAACTGTAGGCACCATCAATAGATCGGAA466710.15891938495736135No Hit
CTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA461770.1572372659505062No Hit
TCGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC458380.15608293732029588No Hit
TTTTGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAA457700.155851390574413No Hit
TTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA429490.14624560571947703No Hit
GTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAA426520.14523429125584145No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGTCTGTAGGCACCATCAA419790.14294265949144164No Hit
TTGGGTTCGTGGCGGCTAGCGTTTCTGTAGGCACCATCAATAGATCGGAA413920.14094386625859956No Hit
GACGACAGTGAAATACCACCACTCTGTAGGCACCATCAATAGATCGGAAG411600.1401538832432344No Hit
GTGGGTTCGTGGCGGCTAGCGTTTAGCTGTAGGCACCATCAATAGATCGG398400.13565915229374292No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATCTGTAGGCACCATCAATAGAT398270.13561488600408886No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTAGCCTGTAGGCACCATCAATAGA395940.1348214978895195No Hit
TACTGCGATCTGTTGAGACTATCCTTTGATTCTGTAGGCACCATCAATAG378600.12891705586950572No Hit
ACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGGAAGAGC378450.12886597938144329No Hit
ACGCCGACGGCGTTGGCTGTTCGTTCTAGCTGTAGGCACCATCAATAGAT366590.12482753172530928No Hit
AGTGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCG360000.12258357134976769No Hit
ACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG335730.11431939558127087No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGATC335430.11421724260514605No Hit
TGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATAG332380.1131786873478772No Hit
GTGGGTTCGTGGCGGCTAGCGTTTCTGTAGGCACCATCAATAGATCGGAA327950.11167022840043422No Hit
TGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAAG313580.106777100844056No Hit
GCAGCGCACCACGGCCCTGTGCGTGCTGTAGGCACCATCAATAGATCGGA312760.10649788270931486No Hit
GCGACGTGGGATCTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC312330.10635146344353597No Hit
TCGACTCTTTACTTATTCGGTTAAAAGCTGTAGGCACCATCAATAGATCG311230.10597690253107833No Hit
CTACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAA309120.10525842659900053No Hit
TACTGCGATCTGTTGAGACTATCCTTTGACTGTAGGCACCATCAATAGAT302640.10305192231470472No Hit
GTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGATCGGAAGAGCAC301370.10261947471577636No Hit
GAGTGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATC301220.10256839822771398No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCAGTCTGTAGGCACCATCAA296740.10104291378425019No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTCTGTAGGCACCATCAATAGATCGG295730.10069899876463001No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)