FastQCFastQC Report
Sat 16 Sept 2023
SRR15906274_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15906274_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39508245
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGGCTGTAGGCACC8992342.2760666792463193No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA8915362.2565821387409137Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGGCTGTAGGCACCA5834861.4768714732836146No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGA3935260.9960604425734426No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGATC3932580.9953821031534051No Hit
ATATGGATAACTGCGGAAATACTGGAGCTAATACATGCCTGTAGGCACCA3604260.9122804619643318No Hit
ACGGTGGCCATGGAAGTCGGTATCCGCTCTGTAGGCACCATCAATAGATC3303000.8360280240238462No Hit
AGTGAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAAT3286900.8319529252691431No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA2445920.619091027708267No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGAT2124240.5376700483658537No Hit
GTGAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAATA2036960.5155784571043335No Hit
TCGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA1958310.4956712200200237No Hit
AAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGATCG1727030.4371315405176818No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATA1711290.4331475619835809No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG1415530.35828723852451555No Hit
TAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAAT1302440.32966283366927585No Hit
AACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAATAGATC1268130.32097857042245237No Hit
TCAACCTGAACTCAGTCGTGATTACCCGCTGAACTTAAGCCTGTAGGCAC1216160.30782435413165027No Hit
TCCGGGGCTGCACGCGTGCTACACTGGTGGAGTCAGCGCTGTAGGCACCA1171020.29639889091504823No Hit
CATATGGATAACTGCGGAAATACTGGAGCTAATACATGCCTGTAGGCACC1103600.2793340984900747No Hit
TTAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGCTGTAGGCACCA1086110.2749071744391582No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA1010130.2556757456576469No Hit
AGAACGTGAAATCGCTGGAGTGGAACCGGAGACAGCTGTAGGCACCATCA919000.23260967425913248No Hit
TTTAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGGCTGTAGGCAC900080.22782080044304676No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGAT897610.2271956144850271No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAAT837480.21197600652724516No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA834500.21122173359003926No Hit
ACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG833520.21097368410062253No Hit
TAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGCTGTAGGCACCAT796910.20170726388884144No Hit
AGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAATA753950.19083358423032964No Hit
TTAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAA742180.18785445924009025No Hit
ATATGGATAACTGCGGAAATACTGGAGCTAATACATGCACTGTAGGCACC725630.18366546020963473No Hit
TAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTTAGTAACGCTGTAGGCA676210.17115667881476387No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGCTGTAGGCACCATCAAT639760.16193075647880587No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGA634230.16053104864566875No Hit
ATGTCCGAAGTCCTTACGATTGAGGCCCTGTAGGCACCATCAATAGATCG621560.15732412310392427No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTCTGTAGGCACCATCAATAGATCG617260.1562357426911775No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG606520.15351732277654956No Hit
CTCTAAGTTAGAATCTCGCCTTGTCAAGGCGAAAATTCTGTAGGCACCAT596840.15106720128924986No Hit
TATATGGATAACTGCGGAAATACTGGAGCTAATACATGCCTGTAGGCACC594250.15041164192436288No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGA591020.14959409105618332No Hit
ATGAGCGTAGGCTCGGCTGGAGCTTCCGTCCTGTAGGCACCATCAATAGA588900.1490574942015268No Hit
TAGTGAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAA574720.14546836995670143No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGAT572140.14481534170905339No Hit
TTGACTCTATCCGGAAAGGGTGTCTGCCCTTTCAACTCTGTAGGCACCAT557100.14100854138167868No Hit
AAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAATAGA556180.14077567859569567No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC548040.13871534916319367No Hit
AAGTTAGAATCTCGCCTTGTCAAGGCGAAAATTCTGTAGGCACCATCAAT547820.13865966458393686No Hit
AGTGAAACGGGAAGAGCCCAGCGCCGAATCGACTGTAGGCACCATCAATA546100.13822431241883815No Hit
ACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAATAGATCG539690.1366018662686738No Hit
TGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGA521380.13196739060416376No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCA518500.13123842883934733No Hit
GTGAAACGGGAAGAGCCCAGCGCCGAATCGACTGTAGGCACCATCAATAG509680.129005983434597No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGTCTGTAGGCACCATCAA507020.1283327062490374No Hit
ACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG503870.12753540431876942No Hit
AGCGCGGTAACGCATTCGAACTTGGTTCTGTAGGCACCATCAATAGATCG492090.12455374821129109No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGAT488770.12371341728796104No Hit
TAGCCAGAGATGGGGACGTTGTTTCCGAAAAGCACCGCGCTGTAGGCACC482440.12211122007570825No Hit
TGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATAG478820.12119495563520982No Hit
ACGGCTCATTAGAGCAGATATCACCTTATCCGGGATCCTGTAGGCACCAT475820.12043562046352603No Hit
TCTAAGTTAGAATCTCGCCTTGTCAAGGCGAAAATTCTGTAGGCACCATC473900.11994964595364842No Hit
TTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA470060.11897769693389317No Hit
CAAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGATC467400.11830441974833356No Hit
TCATATGGATAACTGCGGAAATACTGGAGCTAATACATGCCTGTAGGCAC466540.1180867436657842No Hit
ACCTGAACTCAGTCGTGATTACCCGCTGAACTGTAGGCACCATCAATAGA460480.11655288661898296No Hit
TAGAGATGGGGACGTTGTTTCCGAAAAGCACCGCGCTGTAGGCACCATCA454040.11492284711710175No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAG453100.11468492209664086No Hit
AGCGGAGGAAAAGAAACTAAAAAGGATTCCCTTAGTAACGCTGTAGGCAC430810.10904306177103033No Hit
TAGTCGTGATTACCCGCTGAACTTAAGCCTGTAGGCACCATCAATAGATC425140.10760791829654798No Hit
AGTGTCGTATGTCCGAAGTCCTTACGATTGAGGCCCTGTAGGCACCATCA415980.10528941490567348No Hit
TAAACCAGAGAGGGTGCGAGCCCCGTTCTGGATAGCGGCCTGTAGGCACC414090.10481103374751269No Hit
TCCAGAGATGGGGACGTTGTTTCCGAAAAGCACCGCGCTGTAGGCACCAT412990.10453261085122864No Hit
TGTGAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAAT406180.10280892001150646No Hit
GAGAACGTGAAATCGCTGGAGTGGAACCGGAGACAGCTGTAGGCACCATC405250.10257352610828449No Hit
TTAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGGCCTGTAGGCAC405250.10257352610828449No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)