FastQCFastQC Report
Sat 16 Sept 2023
SRR15906275_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15906275_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39659350
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGGCTGTAGGCACC29867037.530892462937492No Hit
TAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGGCTGTAGGCACCA19988905.040148161782783No Hit
TCCGGGGCTGCACGCGTGCTACACTGGTGGAGTCAGCGCTGTAGGCACCA7654041.9299459017860856No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGA7151871.8033250671027135No Hit
ATATGGATAACTGCGGAAATACTGGAGCTAATACATGCCTGTAGGCACCA6416741.6179639857940182No Hit
TTAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGCTGTAGGCACCA5479411.3816187103419495No Hit
AGTGAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAAT4284301.0802748910408264No Hit
TAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGCTGTAGGCACCAT4096381.0328913610535724No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG3817870.9626658026417477No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGATC3801420.958517978736414No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGAT3581520.9030707765003712No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA3183180.8026304011538263No Hit
GTGAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAATA3181400.8021815788710607No Hit
ATATGGATAACTGCGGAAATACTGGAGCTAATACATGCACTGTAGGCACC2993060.754692146996862No Hit
CATATGGATAACTGCGGAAATACTGGAGCTAATACATGCCTGTAGGCACC2929150.7385774098667779No Hit
CCGGGGCTGCACGCGTGCTACACTGGTGGAGTCAGCGCTGTAGGCACCAT2422810.6109051207344548No Hit
TTTAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGGCTGTAGGCAC2158830.5443432633162167No Hit
AAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGATCG2063290.5202531055097979No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2014450.5079382289422293Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGGTGGCCATGGAAGTCGGTATCCGCTCTGTAGGCACCATCAATAGATC1911720.48203513169025713No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGA1615180.4072633565603067No Hit
TCGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA1613960.40695573679346736No Hit
TAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTTAGTAACGCTGTAGGCA1588970.40065457452025816No Hit
AACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAATAGATC1523190.3840683218459203No Hit
AGAACGTGAAATCGCTGGAGTGGAACCGGAGACAGCTGTAGGCACCATCA1465330.36947907618253956No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGAT1423610.3589594887460334No Hit
TAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAAT1345920.3393701611347639No Hit
ATATGGATAACTGCGGAAATACTGGAGCTAATACATGCTGTAGGCACCAT1319370.33267564899576013No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATA1292350.3258626276023182No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAAT1247750.3146168557981914No Hit
TTAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGGCCTGTAGGCAC1218140.30715077276859054No Hit
AGCGGAGGAAAAGAAACTAAAAAGGATTCCCTTAGTAACGCTGTAGGCAC1203870.3035526300859696No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA1175920.2965051116571502No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAAT1168040.29451819054018785No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA1147420.2893189121859032No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGCTGTAGGCACCATCAAT1048250.26431345949946233No Hit
GTGAAACGGGAAGAGCCCAGCGCCGAATCGACTGTAGGCACCATCAATAG1007550.25405106235982183No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGTTTTTTGCTGTAGGCAC933980.23550058183000983No Hit
CAAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGATC913480.23033156115770934No Hit
CCAGAGATGGGGACGTTGTTTCCGAAAAGCACCGCGCTGTAGGCACCATC912640.23011975738381996No Hit
TCCAGAGATGGGGACGTTGTTTCCGAAAAGCACCGCGCTGTAGGCACCAT894820.22562649160916656No Hit
CATATGGATAACTGCGGAAATACTGGAGCTAATACATGCACTGTAGGCAC892040.22492552197653265No Hit
TATATGGATAACTGCGGAAATACTGGAGCTAATACATGCCTGTAGGCACC880040.22189975377811286No Hit
AGTGAAACGGGAAGAGCCCAGCGCCGAATCGACTGTAGGCACCATCAATA875090.22065162439626468No Hit
AGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAATA863870.21782253113074218No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATA863310.2176813286148159No Hit
TCATATGGATAACTGCGGAAATACTGGAGCTAATACATGCCTGTAGGCAC860740.21703330992565434No Hit
ACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG830650.20944619616811672No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAAT820290.20683394962348095No Hit
CTCTAAGTTAGAATCTCGCCTTGTCAAGGCGAAAATTCTGTAGGCACCAT811160.20453184431918325No Hit
AGAACGTGAAATCGCTGGAGTGGAACCGGAGACAGTCTGTAGGCACCATC803560.20261552446018405No Hit
TAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGGCCTGTAGGCACC792230.19975869498617604No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGTCTGTAGGCACCATCAA788080.19871228348422248No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCA773880.1951317911160924No Hit
TTAGAGTGGTGAAATTTCGAACGCATAGCACCAACTCTGTAGGCACCATC724820.18276144213155285No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG713960.18002312191198294No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAATA710120.1790548760884886No Hit
TCCGGGGCTGCACGCGTGCTACACTGGTGGAGTCAGCGGCTGTAGGCACC676540.17058776807991055No Hit
TTAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAA674980.17019441821411596No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTCTGTAGGCACCATCAATAGA659370.16625839808267154No Hit
GAGAACGTGAAATCGCTGGAGTGGAACCGGAGACAGCTGTAGGCACCATC656610.165562471397035No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAATAG639860.1613390032867407No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTCTGTAGGCACCATCAATAGATCG628250.15841157255476956No Hit
TCTAAGTTAGAATCTCGCCTTGTCAAGGCGAAAATTCTGTAGGCACCATC606760.15299292600609943No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAG573030.14448799589504116No Hit
AAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAATAGA548500.13830282140277134No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGAT541060.1364268451197511No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGCTGTAGGCACCATCAATA536300.13522662373437788No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATAGA536150.13518880163189764No Hit
TAGAGTGGTGAAATTTCGAACGCATAGCACCAACTCTGTAGGCACCATCA527390.1329799908470512No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAA524200.13217564080097127No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAA522130.13165369578674388No Hit
TTAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTTAGTAACGCTGTAGGC514800.12980545571220908No Hit
GCCAGAGATGGGGACGTTGTTTCCGAAAAGCACCGCGCTGTAGGCACCAT501140.1263611229130079No Hit
TCAACCTGAACTCAGTCGTGATTACCCGCTGAACTTCTGTAGGCACCATC499140.12585682821327127No Hit
CCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGAT489880.12352194375349065No Hit
ATAGCACCAACTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG486430.12265203539644498No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGTTTTTTCTGTAGGCACC472750.1192026596502464No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAG465430.11735694104921034No Hit
TTTGATCCTTCGATGTCGGCTCTTCCTATCATTGCGAACTGTAGGCACCA461780.11643660322219099No Hit
AGTGAAACGGGAAGAGCCCAGCGCCGAATCGATCCTGTAGGCACCATCAA460180.11603316746240167No Hit
ACCTGAACTCAGTCGTGATTACCCGCTGAACTGTAGGCACCATCAATAGA455480.11484807491802058No Hit
TATTGCCCTTAAACGAGGAATGCCTAGTAAGTGTGAGTCTGTAGGCACCA449700.11339066323578172No Hit
GAGAACGTGAAATCGCTGGAGTGGAACCGGAGACAGTCTGTAGGCACCAT446020.11246276098826632No Hit
GTTGAAATTCTTCCATGTCCGGGATAGGGTATCTGTAGGCACCATCAATA444590.11210219027795462No Hit
TAGGCAATAAAAATTTACCTCGGCAATTTATCGCTTCTGTAGGCACCATC442950.1116886686241706No Hit
TCAACCTGAACTCAGTCGTGATTACCCGCTGAACTCTGTAGGCACCATCA425090.10718531695552247No Hit
TTAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGACTGTAGGCACC421620.1063103656514794No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTCTGTAGGCACCATCAATAG420170.10594475199417035No Hit
ACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAATAGATCG410870.10359978164039502No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGA410150.10341823554848983No Hit
CTAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGGCTGTAGGCACC406920.10260379960841516No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGAT405750.10230878720906925No Hit
TGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATAG403360.10170615504288395No Hit
TTAGAGTGGTGAAATTTCGAACGCATAGCACCAACCTGTAGGCACCATCA400510.10098753509575925No Hit
TATATGGATAACTGCGGAAATACTGGAGCTAATACATGCACTGTAGGCAC398760.10054627723348972No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)