FastQCFastQC Report
Sat 16 Sept 2023
SRR15906276_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15906276_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38364534
Total Bases5.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGA16123254.202644557079724No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA13899903.6231119085142547No Hit
ACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG12079143.14851732592399No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA10761042.8049447961494853Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG10486142.733290074629865No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG7357161.9176982574583077No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGAT6430221.6760844794830558No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAG4676931.219076452225381No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA4615701.203116399120083No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA3847991.0030071002556684No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAG3785570.9867368648345892No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG3669130.9563859162214768No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAATA3536840.9219035476880809No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGCTGTAGGCACCATCAAT3502470.9129447525675667No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAAT3495010.911000248302247No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGATC3202340.8347136446385612No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCA2904320.7570325238408995No Hit
TCGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA2801510.730234335701823No Hit
AGTGAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAAT2645190.6894883696489054No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGA2489190.6488258139666182No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATA2150980.5606688719326032No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATAGA1975030.5148062009563312No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGA1923520.5013797378589299No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1846030.4811813952959783Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACTGCGATCTGTTGAGACTATCCTTTGATTCTGTAGGCACCATCAATAGA1803090.4699887661870205No Hit
CCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA1765310.46014112930447687No Hit
TGCAGCGCACCACGGCCCTGTGCGTGCTGTAGGCACCATCAATAGATCGG1735350.452331833354212No Hit
GTGAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAATA1727510.45028827927376885No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAAT1644160.4285624842986494No Hit
TAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGG1624090.42333109011567815No Hit
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATA1584490.4130090567501746No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGTCTGTAGGCACCATCAA1513330.39446067558125425No Hit
CTAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCG1494870.38964893982551696No Hit
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAAT1435340.3741320043141929No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGA1431110.3730294234774232No Hit
TGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGA1419280.36994584633818306No Hit
TTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAAT1415330.3689162495757149No Hit
TACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCG1277060.33287514974116456No Hit
AACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAATAGATC1224200.3190967991426665No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATA1140370.29724588861160156No Hit
TAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAAT1115330.29071902710977804No Hit
TTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAA1078950.28123631059874205No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAA1043230.27192562797713116No Hit
TGTGAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAAT1037950.27054935686173065No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGAT1005620.26212230285398486No Hit
TAGTGAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAA1004400.26180430081595674No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTCTGTAGGCACCATCAATAGATCG1003290.2615149710928328No Hit
TCCAGTTGGTACGTCTGGTTCTGTAGGCACCATCAATAGATCGGAAGAGC976710.2545866971823507No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGCTGTAGGCACCATCAATA953570.24855508475614482No Hit
ACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAATAGATCG953300.24848470725592547No Hit
TTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATA890200.23203722479725677No Hit
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATAG867480.22611508848250314No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAAT857910.22362059708583973No Hit
ACTGCGATCTGTTGAGACTATCCTTTGACTGTAGGCACCATCAATAGATC841070.21923112633141847No Hit
TACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATC839000.21869156549640356No Hit
TCTCCAGTTGGTACGTCTGGTTCTGTAGGCACCATCAATAGATCGGAAGA834770.2175889846596338No Hit
AAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGATCG811940.21163817602997603No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC777020.20253601933494098No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAATAG774330.2018348509068297No Hit
TGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATAG761010.1983628942293421No Hit
CAAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGATC760030.19810744996928675No Hit
TCTAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATC754710.19672075255755747No Hit
ACGGTGGCCATGGAAGTCGGTATCCGCTCTGTAGGCACCATCAATAGATC745170.19423408088314068No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAATA728710.18994365994384294No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAAT710780.1852700726144621No Hit
TCTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG706980.1842795744632269No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTCTGTAGGCACCATCAATAGATCGG682630.17793256657307502No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATCTGTAGGCACCATCAATAGAT666230.17365778507827048No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGAT664220.1731338636877487No Hit
ACAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG662870.17278197618665198Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGA659520.17190877386911568No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCCTGTAGGCACCATCAATAG659520.17190877386911568No Hit
AGTGAAACGGGAAGAGCCCAGCGCCGAATCGACTGTAGGCACCATCAATA655950.17097822692177103No Hit
CTAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGG636620.1659397192208825No Hit
AAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAATAGA629130.16398739523331626No Hit
TTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGATCGGA610010.1590036255881539No Hit
ACCTGAACTCAGTCGTGATTACCCGCTGAACTGTAGGCACCATCAATAGA602610.15707476076732746No Hit
TTCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT587340.15309452214381125Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAG582390.1518042679731233No Hit
GTGAAACGGGAAGAGCCCAGCGCCGAATCGACTGTAGGCACCATCAATAG565810.1474825681448392No Hit
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA562360.14658330008648093No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGAT549720.1432885904465828No Hit
TGCGGTCCGGATCGTATGGGTTATCAATTCTGTAGGCACCATCAATAGAT549190.14315044202022628No Hit
CCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGAT546120.14235022377699155No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTCTGTAGGCACCATCAATAG540050.1407680333090974No Hit
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGCTGTAGGCACCATCAAT534850.1394126147863545No Hit
TTAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAA522160.13610487227604537No Hit
ACTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA521510.1359354449607025No Hit
AGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAATA519020.13528640801423522No Hit
CTCCAGTTGGTACGTCTGGTTCTGTAGGCACCATCAATAGATCGGAAGAG506140.13192914059636435No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGCTGTAGGCACCATCAATAG494400.12886902262386402No Hit
TCAAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGAT493500.12863443095646618No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGCTGTAGGCACCATCAATAGAT479500.12498522724138915No Hit
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAAT427220.11135805793965854No Hit
TCCAGTTGGTACGTCTGGTTCCTGTAGGCACCATCAATAGATCGGAAGAG420700.1096585716380655No Hit
TCGGCCTGGGAATCCTGGATGTTGTAAGCTTCTGTAGGCACCATCAATAG415200.10822495589285666No Hit
GTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAGAT414740.10810505348507556No Hit
GTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC400150.10430206189914883No Hit
ACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGC389720.10158340513141642No Hit
ACCTGAACTCAGTCGTGATTACCCGCTGACTGTAGGCACCATCAATAGAT386140.10065025160998957No Hit
GTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA385800.10056162809119486No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)