FastQCFastQC Report
Sat 16 Sept 2023
SRR15906279_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15906279_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42732217
Total Bases6.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG17206754.026645750675655No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGA14742703.4500199229073463No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA14501593.3935964520633224No Hit
ACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG14311373.3490820286717162No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC6960721.6289161875219345No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5930361.387796004124944Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG5902051.3811710260668197No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG4477781.0478698074569825No Hit
TGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGA4052740.9484038705504093No Hit
TAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA3889780.9102687089696282Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAAT3826020.8953478823717477No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA3752670.8781828473818711No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGAT3734640.8739635483925395No Hit
TGCAGCGCACCACGGCCCTGTGCGTGCTGTAGGCACCATCAATAGATCGG3662280.8570301887215448No Hit
TAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGA3345080.782800480489931No Hit
TAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGG3013660.7052430722234702No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAG2906410.6801449126779451No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGAT2659370.6223337300753669No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTCTGTAGGCACCATCAATAGA2452730.5739767726069537No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAG2401860.5620724054640086No Hit
CTAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCG2391400.5596246036099648No Hit
TCGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC2282370.5341098965213998No Hit
TTGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAA2079680.4866773001737775No Hit
ATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2026970.4743423445593754No Hit
CTAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGG2003710.4688991446430219No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGATC1937370.45337455812320715No Hit
ACTGCGATCTGTTGAGACTATCCTTTGACTGTAGGCACCATCAATAGATC1853250.43368917648246524No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATA1789890.41886195607403187No Hit
TACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATC1785570.4178510092280024No Hit
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATA1736220.4063023456049566No Hit
ATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1697100.3971476602770223No Hit
CCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA1692320.3960290663131286No Hit
TTCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1688320.39509300441865675Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGCTGTAGGCACCATCAAT1608480.3764092090049997No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATAGA1555790.36407893370006994No Hit
TTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAAT1539340.3602293791590547No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1430590.33478019640310264Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAATA1417210.33164906936609445No Hit
ACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGGAAGAGC1408720.3296622779950781No Hit
TCGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGAT1398510.3272729800094388No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATCTGTAGGCACCATCAATAGAT1376030.32201231216250725No Hit
TACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCG1373510.32142259316899No Hit
TCTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG1279390.2993970567920686No Hit
TCTAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATC1269010.2969679761759143No Hit
AGTAGAGCAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1266950.29648590430026134Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1259970.29485247629440803Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATA1257980.29438678550190833No Hit
TCTAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCG1254410.29355135026109225No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA1237520.2895988289116851No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGA1210440.28326168988611095No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGA1196750.28005801805228125No Hit
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATAG1186160.2775797941866672No Hit
TCGACTCTTTACTTATTCGGTTAAACTGTAGGCACCATCAATAGATCGGA1166680.2730211727605895No Hit
TTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGATCGGA1159320.27129881887476137No Hit
TCGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA1147260.2684765922629289No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCA1136640.2659913479331063No Hit
ACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCA1123090.2628204382655831No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAAT1112350.2603071120789263No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGA1086780.25432333641851534No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTCTGTAGGCACCATCAATAGATCG1074730.25150344996141905No Hit
TACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGAT1067000.2496945103503523No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATA1064580.24912819290419685No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAAT1035720.2423745063355828No Hit
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAAT1015210.23757484897167866No Hit
TCGACTCTTTACTTATTCGGTTAACTGTAGGCACCATCAATAGATCGGAA1011860.23679089713505858No Hit
ACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGC843630.19742247400831087No Hit
TCTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG833490.19504955710582486No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGCTGTAGGCACCATCAATA791390.18519750566650917No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAATA758080.17740245024029527No Hit
CTAAATCACTTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC755720.17685017372255693No Hit
TTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA755260.17674252660469267No Hit
AAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGATCG743470.17398348417073703No Hit
TCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG734330.17184458274186895Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTCTGTAGGCACCATCAATAGATCG732400.17139293287778634No Hit
TAAATCACTTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT722060.16897321288057673Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAAG698850.16354171373790413No Hit
TTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAA695100.1626641557118368No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCCTGTAGGCACCATCAATAG693740.1623458946677164No Hit
AGTAGAGCAGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG691530.16182872047102073Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGATC691340.16178425753103332No Hit
TTGACGACAGTGAAATACCACCACTCTGTAGGCACCATCAATAGATCGGA666240.1559104691432228No Hit
GCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGAA665900.15583090388219267No Hit
ACGACTTGGTCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT664250.15544477835072307Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTAGAGCAGTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC647400.1515016176202606No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAATAG626890.14670196025635646No Hit
AAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAGATCGG619890.1450638519410308No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTCTGTAGGCACCATCAATAGATCGG614830.14387973364452397No Hit
TCAAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGAT611630.14313088412894653No Hit
GTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA611240.14303961809423554No Hit
GTGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGG582940.13641698019084758No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAAT578760.13543879551112453No Hit
GCAGCGCACCACGGCCCTGTGCGTGCTGTAGGCACCATCAATAGATCGGA575350.13464080274608733No Hit
TCCAGTTGGTACGTCTGGTTCAGGGCTGTAGGCACCATCAATAGATCGGA560830.1312428980691547No Hit
TTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC560210.13109780847551158No Hit
ACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG548370.12832706526787507Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC538330.12597754991275084No Hit
ACAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG537470.1257762966054394Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAA527590.12346422372609407No Hit
ACGGTGGCCATGGAAGTCGGTATCCGCTCTGTAGGCACCATCAATAGATC523260.12245093672532835No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGAT514700.12044776427115869No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGAT512810.12000547502602076No Hit
TATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT498800.11672691824063329No Hit
CAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGATC490510.11478692996434048No Hit
AGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGATCG479370.11217999758823653No Hit
AAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATAGATCGGA477560.11175642958098804No Hit
TGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATAG475490.11127201755059889No Hit
TATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG474590.11106140362434273No Hit
TCCAGTTGGTACGTCTGGTTCCTGTAGGCACCATCAATAGATCGGAAGAG469700.10991706795835095No Hit
CTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCGG457000.106945071443403No Hit
CAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA456420.10680934246870459Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGAT451340.1056205438627254No Hit
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAAT445860.10433813906729904No Hit
TCTCCAGTTGGTACGTCTGGTTCCTGTAGGCACCATCAATAGATCGGAAG439650.10288490297613156No Hit
ACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT439020.10273747322775226No Hit
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA438370.1025853631699006No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)