FastQCFastQC Report
Sat 16 Sept 2023
SRR15906292_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15906292_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37322214
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGA12135273.251487170616406No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC9649262.585393245963383No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGA8561262.2938778497974424No Hit
TAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGA7429161.9905464343567614No Hit
ACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG7193461.9273936964189744No Hit
ACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG6511681.744719646053152No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG5584041.4961706183882875No Hit
CTAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGG5465091.464299518779888No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGAT5018101.3445343837318977No Hit
TTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA4499081.205469750535164No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATA3495680.9366218199166856No Hit
TAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGG3392740.9090403907978235No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA3059280.8196941371163028No Hit
CTAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCG3013530.8074360218823031No Hit
GTGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGG2984400.799631018674294No Hit
TCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAG2849340.7634434548818567No Hit
TCGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC2630850.7049019117676139No Hit
TGCAGCGCACCACGGCCCTGTGCGTGCTGTAGGCACCATCAATAGATCGG2567260.6878638014347166No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTCTGTAGGCACCATCAATAGATCG2478940.6641996104518344No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGATC2168840.5811123638056412No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCCTGTAGGCACCATCAATAG2112200.5659364152405321No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGAT2104040.5637500497692876No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCA2075480.5560977706199316No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAAT1785980.478530025040851No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA1771200.47456991699367035No Hit
TACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGAT1765620.47307482884053986No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGCTGTAGGCACCATCAAT1709940.4581560997426359No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAAT1653410.4430096242414772No Hit
AAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAA1616260.4330557667345244No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAG1574450.42185332306384615No Hit
TCGACTCTTTACTTATTCGGTTAAACTGTAGGCACCATCAATAGATCGGA1568000.4201251297685609No Hit
TCGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGAT1486690.398339176770167No Hit
CTTGGTCAAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1453880.3895481656045378Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGAA1435010.3844921954522848No Hit
TCGACTCTTTACTTATTCGGTTAACTGTAGGCACCATCAATAGATCGGAA1390670.37261187131074275No Hit
TGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAAG1385290.37117037054661334No Hit
AGTGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCG1382470.37041478836169794No Hit
TGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATAG1343380.35994113318143456No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTCTGTAGGCACCATCAATAGA1239040.33198459233956484No Hit
TGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCA1186720.3179661313768792No Hit
ACTGCGATCTGTTGAGACTATCCTTTGACTGTAGGCACCATCAATAGATC1177660.3155386226551297No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAA1137430.3047595193575601No Hit
TACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGA1117280.2993605899157001No Hit
CGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAGA1104150.2958425778277784No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAATA1079000.2891039636608911No Hit
TTGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATC1051790.2818133993872925No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGA1041100.2789491534451842No Hit
TACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATC979780.26251925997744935No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG956040.25615843690301976No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGTCTGTAGGCACCATCAA937490.2511882065731684No Hit
ACGGTGGCCATGGAAGTCGGTATCCGCTCTGTAGGCACCATCAATAGATC919130.24626888426286822No Hit
GACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAAGA896390.24017599813344406No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATA881390.23615694395836218No Hit
TACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCG851910.22825816281960123No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGAT842360.2256993649947991No Hit
TTGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGG818730.21936801498432007No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTCTGTAGGCACCATCAATAGATCGG812980.21782737755053866No Hit
TGTTGACTCTGAATAAAGCAGTTTACTGTCCTGTAGGCACCATCAATAGA806830.2161795653387551No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAATA779680.20890507728185687No Hit
CTCTAAGTTAGAATCTCGCCTTGTCAAGGCGAAAATTCTGTAGGCACCAT763870.20466899418132056No Hit
TCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAG761990.2041652727247103No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATG757950.20308280746688823TruSeq Adapter, Index 25 (97% over 43bp)
AAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGGA734570.19681844169266055No Hit
TTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATA730990.195859227429541No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATCTGTAGGCACCATCAATAGAT722480.19357908402754456No Hit
ATATGGATAACTGCGGAAATACTGGAGCTAATACATGCCTGTAGGCACCA689610.1847719966452151No Hit
TGTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGG680000.18219712260371262No Hit
GGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAGAG663140.17767970571092057No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAAT654250.17529774626982203No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATAGA652600.17485565031056305No Hit
TTTGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCAT650510.17429566209550162No Hit
TCGCCGACGGCGTTGGCTGCTCGTTCTCTGTAGGCACCATCAATAGATCG645500.17295329800102427No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCC636030.17041593513182257TruSeq Adapter, Index 25 (97% over 41bp)
GTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA615670.1649607389315114No Hit
GAGTGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATC600110.16079164006722646No Hit
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA576410.15444153447059705No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAATAG576220.15439062645104604No Hit
TACGCCGACGGCGTTGGCTGCTCGTTCTCTGTAGGCACCATCAATAGATC573070.15354662507427883No Hit
GTGCAGCGCACCACGGCCCTGTGCGTGCTGTAGGCACCATCAATAGATCG565320.15147011375048652No Hit
GGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAGAGC560840.15026975623686206No Hit
GTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATAGA533740.1430086650272141No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA527590.1413608528154305Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAAT518710.13898157274378203No Hit
ACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCA509650.13655406402203257No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCAGCTGTAGGCACCATCAAT491260.13162670360338216No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAA465040.124601396905339No Hit
TCTAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCG457470.12257311423164768No Hit
ACGCCGACGGCGTTGGCTGCTCCTGTAGGCACCATCAATAGATCGGAAGA451630.1210083624728158No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA450610.12073506678891022No Hit
ACGCCGACGGCGTTGGCTGCTCGCTGTAGGCACCATCAATAGATCGGAAG447020.11977317315634063No Hit
TCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGAA441390.11826468815595989No Hit
TAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGGCTGTAGGCACCA437940.11734030569569105No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAATA433350.11611047511811598No Hit
TCCAGTTGGTACGTCTGGTTCAGGGCTGTAGGCACCATCAATAGATCGGA422810.11328641971775844No Hit
GCAGCGCACCACGGCCCTGTGCGTGCTGTAGGCACCATCAATAGATCGGA409860.10981663627993773No Hit
CCGACTCTTTACTTATTCGGTTAAACTGTAGGCACCATCAATAGATCGGA398630.10680770438752643No Hit
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCA393390.10540371479569781No Hit
CTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCGG388710.10414976989307224No Hit
CTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGGA384110.10291725994604714No Hit
TGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGAA378910.10152398783201874No Hit
GTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAA375690.10066123086910118No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)