FastQCFastQC Report
Sat 16 Sept 2023
SRR15906293_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15906293_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28205476
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGA10520143.7298218260879556No Hit
TGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGA7136632.530228527254778No Hit
ACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG7073812.5079562564375797No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG6679012.3679834369751465No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC6033322.139059805266183No Hit
ACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG5665092.0085071423719283No Hit
TAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGA5599251.98516415748488No Hit
CTAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGG3276661.1617105841433062No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGAT3014991.06893781902493No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGAT2827371.002418821082828No Hit
TAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGG2777340.9846811307137663No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGATC2682710.9511309080548755No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATA2525380.8953509595087138No Hit
TTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA2289920.8118707161687326No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCCTGTAGGCACCATCAATAG2170260.76944633020907No Hit
ACGGTGGCCATGGAAGTCGGTATCCGCTCTGTAGGCACCATCAATAGATC2119250.7513611895789314No Hit
CTAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCG2052590.7277274810040434No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA1874870.6647184397809843No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGCTGTAGGCACCATCAAT1784460.632664380491221No Hit
TCGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC1670820.5923743318496025No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCA1662060.5892685519648738No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAAT1631890.5785720474988616No Hit
GTGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGG1545700.5480141515782254No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGA1498990.5314535376038326No Hit
CTCTAAGTTAGAATCTCGCCTTGTCAAGGCGAAAATTCTGTAGGCACCAT1410530.5000908334253958No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAA1379320.48902560623334274No Hit
TTGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATC1286700.4561880111507425No Hit
AAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAA1212700.4299519710286045No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA1203520.4266972838891285No Hit
TGCAGCGCACCACGGCCCTGTGCGTGCTGTAGGCACCATCAATAGATCGG1180910.4186811100085671No Hit
CTTGGTCAAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1137470.4032798453747067Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAAT1110040.39355478347537903No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTCTGTAGGCACCATCAATAGATCG1092530.3873467691167488No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAG1081980.3836063606939305No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGAT1076160.38154293159243263No Hit
GCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGAA1020410.3617772662301462No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGTCTGTAGGCACCATCAA1012530.35898348249822126No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTCTGTAGGCACCATCAATAGA1003280.355703977482954No Hit
TCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAG945540.3352327753660318No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAATA936580.33205608726475666No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATA914350.32417463899563337No Hit
TACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGAT843290.2989809496567263No Hit
TCGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGAT828210.2936344701291338No Hit
ACTGCGATCTGTTGAGACTATCCTTTGACTGTAGGCACCATCAATAGATC799940.2836115937203116No Hit
TGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATAG787310.27913373984541157No Hit
TACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCG776640.2753507864926655No Hit
TCTAAGTTAGAATCTCGCCTTGTCAAGGCGAAAATTCTGTAGGCACCATC709010.2513731730675277No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG688930.24425398812627733No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTCTGTAGGCACCATCAATAGATCGG685490.24303436680168067No Hit
TACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATC685360.24298827646092552No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA676290.23977258884054997No Hit
TGTTGACTCTGAATAAAGCAGTTTACTGTCCTGTAGGCACCATCAATAGA658620.2335078479086827No Hit
AGTGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCG627360.22242489366249307No Hit
TCCAGTTGGTACGTCTGGTTCTGTAGGCACCATCAATAGATCGGAAGAGC597320.21177447953723594No Hit
AAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGGA591480.20970395961408345No Hit
TACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGA569630.20195723695639814No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAATAG561320.19901100055889856No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAATA547760.19420342347705816No Hit
TCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAG544910.1931929813912731No Hit
TGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCA536040.19004820198744385No Hit
CTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGGA527440.18699914867595216No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATCTGTAGGCACCATCAATAGAT516350.18306728806845876No Hit
GTGAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAATA490310.17383503827412802No Hit
GTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA485860.17225733045597244No Hit
TCGACTCTTTACTTATTCGGTTAAACTGTAGGCACCATCAATAGATCGGA485430.17210487779039788No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATAGA483070.1712681608351513No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAAT477310.1692260041986173No Hit
CTCCAGTTGGTACGTCTGGTTCTGTAGGCACCATCAATAGATCGGAAGAG476310.16887146311588572No Hit
AGTGAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAAT474490.16822619834531422No Hit
GTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATAGA462300.1639043425468161No Hit
CTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCGG455950.16165300667147048No Hit
TTCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT445370.1579019620161702Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTCTGTAGGCACCATCAATAGATCG431820.1530979303451571No Hit
CCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGAT421550.1494567934255036No Hit
GTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAGAT419550.14874771126004044No Hit
TCGACTCTTTACTTATTCGGTTAACTGTAGGCACCATCAATAGATCGGAA419100.1485881677728112No Hit
AACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAATAGATC405720.1438444080858625No Hit
TTTGCTTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCAT403040.1428942379841418No Hit
GTTGACTCTGAATAAAGCAGTTTACTGTCCTGTAGGCACCATCAATAGAT401180.14223479157026103No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAATA400800.14210006595882305No Hit
TTGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGG389410.13806184302651017No Hit
TCCAGTTGGTACGTCTGGTTCAGGGCTGTAGGCACCATCAATAGATCGGA388060.1375832125648225No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA374950.13293517897021132Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGAACGTGAAATCGCTGGAGTGGAACCGGAGACAGCTGTAGGCACCATCA354940.1258408119047521No Hit
TGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGAA346470.1228378489340155No Hit
TTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAAT337630.1197037057626682No Hit
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA329980.11699146647977152No Hit
GTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAATAGA324800.11515494367122187No Hit
TGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGATCGGAA320120.113495691404038No Hit
TTCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATA315060.11170171352541612No Hit
TCGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA304800.10806412201658996No Hit
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCA291950.10350826910348898No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCAGCTGTAGGCACCATCAAT283690.10057975976012601No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)