FastQCFastQC Report
Sat 16 Sept 2023
SRR15906294_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15906294_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32935697
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGGCTGTAGGCACC16447804.993912835668849No Hit
TAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGGCTGTAGGCACCA12496083.794083969135373No Hit
TCCGGGGCTGCACGCGTGCTACACTGGTGGAGTCAGCGCTGTAGGCACCA8158252.477023637908741No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGA7908372.401154589198461No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5348111.6238034980708014Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATATGGATAACTGCGGAAATACTGGAGCTAATACATGCCTGTAGGCACCA4556061.3833197457457787No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG4424461.3433630993144006No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGATC4232371.2850403621335234No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGAT3634341.10346533732078No Hit
CCGGGGCTGCACGCGTGCTACACTGGTGGAGTCAGCGCTGTAGGCACCAT3089620.938076397775945No Hit
AGTGAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAAT3036030.9218052983666932No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA2999770.9107959670627284No Hit
TTAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGCTGTAGGCACCA2922200.8872440136912846No Hit
ATATGGATAACTGCGGAAATACTGGAGCTAATACATGCACTGTAGGCACC2496040.7578524905666942No Hit
TAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGCTGTAGGCACCAT2230160.6771254909225087No Hit
GTGAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAATA2181800.6624423342247774No Hit
AAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGATCG2154890.654271867997814No Hit
ACGGTGGCCATGGAAGTCGGTATCCGCTCTGTAGGCACCATCAATAGATC1950120.5920992047018163No Hit
CATATGGATAACTGCGGAAATACTGGAGCTAATACATGCCTGTAGGCACC1803940.5477157504819163No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGA1676270.5089523382486789No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGAT1632730.4957326392697868No Hit
ACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG1583790.4808733818507014No Hit
TCGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA1442490.43797160266564267No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAAT1343680.4079707194294385No Hit
AACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAATAGATC1260490.3827124107924602No Hit
TTTAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGGCTGTAGGCAC1244040.377717829988538No Hit
CAAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGATC1172740.35606958613931866No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA1133260.3440825922099053No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA1093870.332122924254495No Hit
ATATGGATAACTGCGGAAATACTGGAGCTAATACATGCTGTAGGCACCAT1086510.3298882668248982No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATA996090.3024347716096611No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGCTGTAGGCACCATCAAT929960.28235625315596025No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAATAG897840.27260391665614364No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAAT855720.2598153608226357No Hit
ACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG848030.25748050815502704No Hit
AGAACGTGAAATCGCTGGAGTGGAACCGGAGACAGCTGTAGGCACCATCA822480.24972296775744568No Hit
TAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAAT821820.24952257728142205No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTCTGTAGGCACCATCAATAGATCG817100.24808948175591974No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAAT809150.2456756873856351No Hit
CTAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCG786010.23864987584747335No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATA761380.23117166762859154No Hit
CCAGAGATGGGGACGTTGTTTCCGAAAAGCACCGCGCTGTAGGCACCATC761010.23105932751324498No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG720850.21886587066914054No Hit
TTCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT696080.2113451553795871Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAATA647250.19651929637317225No Hit
GTGAAACGGGAAGAGCCCAGCGCCGAATCGACTGTAGGCACCATCAATAG642060.19494349853898643No Hit
TCCAGAGATGGGGACGTTGTTTCCGAAAAGCACCGCGCTGTAGGCACCAT638030.19371990214750884No Hit
AGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAATA611670.18571642798389842No Hit
CTCTAAGTTAGAATCTCGCCTTGTCAAGGCGAAAATTCTGTAGGCACCAT601810.18272271572087878No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGTCTGTAGGCACCATCAA598990.1818665018687778No Hit
AGTGAAACGGGAAGAGCCCAGCGCCGAATCGACTGTAGGCACCATCAATA585370.17773117113629022No Hit
TGCAGCGCACCACGGCCCTGTGCGTGCTGTAGGCACCATCAATAGATCGG585220.17768562784628483No Hit
CATATGGATAACTGCGGAAATACTGGAGCTAATACATGCACTGTAGGCAC574800.17452188730057847No Hit
TTAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGGCCTGTAGGCAC562390.1707539391074675No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGCTGTAGGCACCATCAATA554540.1683705069305198No Hit
TATATGGATAACTGCGGAAATACTGGAGCTAATACATGCCTGTAGGCACC545330.16557414892419006No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGAT545320.1655711127048564No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAATA537170.16309659394789794No Hit
TAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGGCCTGTAGGCACC506030.15364180694278307No Hit
TCATATGGATAACTGCGGAAATACTGGAGCTAATACATGCCTGTAGGCAC488430.1482980609154863No Hit
AGAACGTGAAATCGCTGGAGTGGAACCGGAGACAGTCTGTAGGCACCATC481270.14612412787256332No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATAGA480420.14586604922919955No Hit
TCCGGGGCTGCACGCGTGCTACACTGGTGGAGTCAGCGGCTGTAGGCACC462510.14042818040255836No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAG452140.13727962095352042No Hit
AGCGGAGGAAAAGAAACTAAAAAGGATTCCCTTAGTAACGCTGTAGGCAC450020.13663594245477786No Hit
ACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAATAGATCG448100.13605298834270912No Hit
GCCAGAGATGGGGACGTTGTTTCCGAAAAGCACCGCGCTGTAGGCACCAT447470.13586170652468657No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCA428340.13005341893933503No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTCTGTAGGCACCATCAATAG423120.12846851244714816No Hit
AGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA421820.1280738039337683Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCTAAGTTAGAATCTCGCCTTGTCAAGGCGAAAATTCTGTAGGCACCATC420400.1276426607883841No Hit
CCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGAT416860.12656783914425737No Hit
ATAGCACCAACTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG413800.1256387560281478No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAG411620.12497686021340311No Hit
AAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAATAGA410350.12459126035802431No Hit
TTAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAA410270.12456697060335477No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTCTGTAGGCACCATCAATAGATCGG408990.12417833452864227No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGCTGTAGGCACCATCAATAG407370.12368646699658428No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGA395710.12014623525350018No Hit
TAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTTAGTAACGCTGTAGGCA387660.11770207868987863No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGAT383640.11648151851773472No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAA377300.11455655546017442No Hit
CCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGATCG374570.11372766758207667No Hit
GAGAACGTGAAATCGCTGGAGTGGAACCGGAGACAGCTGTAGGCACCATC361920.10988685012495712No Hit
CGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGAT359480.10914601260753644No Hit
TCAAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGAT359130.10903974493085725No Hit
AGGGCACCACAAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCAT344800.10468884262567754No Hit
AGTGAAACGGGAAGAGCCCAGCGCCGAATCGATCCTGTAGGCACCATCAA341300.10362616585888557No Hit
TCAACCTGAACTCAGTCGTGATTACCCGCTGAACTTAAGCCTGTAGGCAC332790.10104234320591425No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)