FastQCFastQC Report
Sat 16 Sept 2023
SRR15906295_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15906295_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31099261
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGA12068813.880738516584044No Hit
TTAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGGCTGTAGGCACC11971163.8493390566418926No Hit
TAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGGCTGTAGGCACCA8367922.6907134545737277No Hit
TCCGGGGCTGCACGCGTGCTACACTGGTGGAGTCAGCGCTGTAGGCACCA7206402.3172254800524037No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG7085242.278266354946505No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGAT4355091.400383758315029No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA4354691.400255137895399No Hit
AGTGAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAAT3471351.1162162342056938No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGATC3219871.0353525763843714No Hit
ATATGGATAACTGCGGAAATACTGGAGCTAATACATGCCTGTAGGCACCA2892950.9302311074208484No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2691980.8656089930882923Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTGAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAATA2486000.7993759079998717No Hit
TTAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGCTGTAGGCACCA2334140.7505451656873776No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA2195620.7060039143695408No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATA2114580.6799454173525217No Hit
CCGGGGCTGCACGCGTGCTACACTGGTGGAGTCAGCGCTGTAGGCACCAT2101530.675749176162096No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGA2009520.6461632641367265No Hit
ATATGGATAACTGCGGAAATACTGGAGCTAATACATGCACTGTAGGCACC1955670.6288477401440504No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAATAG1947760.626304271345869No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGCTGTAGGCACCATCAAT1879300.6042908865262103No Hit
TCGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA1833710.5896313741988918No Hit
TAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGCTGTAGGCACCAT1722840.5539810093879722No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAAT1716010.5517848157227916No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAAT1452700.46711720899091463No Hit
TTTAGAGTGGTGAAATTTCGAACGCATAGCACCAACTGGGCTGTAGGCAC1322670.42530592607972256No Hit
ACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG1269380.4081704706745282No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAATA1264150.4064887586878672No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGAT1237480.39791299220904314No Hit
ACGGTGGCCATGGAAGTCGGTATCCGCTCTGTAGGCACCATCAATAGATC1236510.3976010876914407No Hit
AAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGATCG1198360.38533391516923826No Hit
CAAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGATC1174080.3775266556977029No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG1164540.3744590586895296No Hit
CATATGGATAACTGCGGAAATACTGGAGCTAATACATGCCTGTAGGCACC1158820.37261978668882195No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAAT1112100.35759692167604884No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA1102690.35457112630425525No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGTCTGTAGGCACCATCAA1006780.32373116518749434No Hit
AACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAATAGATC979500.31495925256873464No Hit
ATAGCACCAACTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG943130.3032644409138854No Hit
ATATGGATAACTGCGGAAATACTGGAGCTAATACATGCTGTAGGCACCAT938620.30181424568255816No Hit
CCAGAGATGGGGACGTTGTTTCCGAAAAGCACCGCGCTGTAGGCACCATC816340.26249498340169564No Hit
GTGAAACGGGAAGAGCCCAGCGCCGAATCGACTGTAGGCACCATCAATAG796330.25606074690970954No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAA780110.25084518889371676No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATA760370.24449777118498087No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGCTGTAGGCACCATCAATAG752880.24208935382741087No Hit
CCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGAT734270.23610528880412948No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAATA724580.23298945913859498No Hit
AGTGAAACGGGAAGAGCCCAGCGCCGAATCGACTGTAGGCACCATCAATA721710.23206660762775036No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATAGA656900.2112268841372147No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAG615060.19777318824392645No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTCTGTAGGCACCATCAATAGATCG609220.1958953301173298No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAG607340.1952908141450692No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGAT600160.1929820776127124No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTCTGTAGGCACCATCAATAGA581620.18702052116286622No Hit
AGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAATA570220.18335483920341386No Hit
CGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGAT567050.18233552237784686No Hit
TAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAAT553790.17807175546711543No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTCTGTAGGCACCATCAATAG538590.17318417952117895No Hit
CTAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCG531570.17092689115667412No Hit
ACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG519270.16697181325305446No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAA498690.16035429266309575No Hit
TCCAGAGATGGGGACGTTGTTTCCGAAAAGCACCGCGCTGTAGGCACCAT487760.15683973969670856No Hit
TCCGGGGCTGCACGCGTGCTACACTGGTGGAGTCAGCGGCTGTAGGCACC486170.15632847352867965No Hit
CATATGGATAACTGCGGAAATACTGGAGCTAATACATGCACTGTAGGCAC486150.15632204250769818No Hit
AAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAATAGA477380.15350203980731247No Hit
AGAACGTGAAATCGCTGGAGTGGAACCGGAGACAGCTGTAGGCACCATCA461630.1484376107843849No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGCTGTAGGCACCATCAATA461100.14826718872837524No Hit
TGCAGCGCACCACGGCCCTGTGCGTGCTGTAGGCACCATCAATAGATCGG454820.1462478481401857No Hit
AGAACGTGAAATCGCTGGAGTGGAACCGGAGACAGTCTGTAGGCACCATC451550.1451963762097112No Hit
TCCCGGTGTCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAG433860.1395081381515786No Hit
AGTGAAACGGGAAGAGCCCAGCGCCGAATCGATCCTGTAGGCACCATCAA428320.1377267453397044No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGA422490.1358521027235985No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAAT405310.13032785570049396No Hit
GCCAGAGATGGGGACGTTGTTTCCGAAAAGCACCGCGCTGTAGGCACCAT404000.12990662382620602No Hit
TCAAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGAT374310.12035977317917618No Hit
TACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGA373450.12008323927697188No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGA372240.11969416250759142No Hit
ACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGC366990.11800601949994889No Hit
ACCTGAACTCAGTCGTGATTACCCGCTGAACTGTAGGCACCATCAATAGA366220.11775842519216131No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGTTTTTTGCTGTAGGCAC359250.1155172143801102No Hit
CCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA358790.11536930089753579No Hit
AGGGCACCACAAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCAT356120.11451075959650617No Hit
TCATATGGATAACTGCGGAAATACTGGAGCTAATACATGCCTGTAGGCAC349220.11229205735789029No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCA347920.11187404099409308No Hit
CCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGATC341060.10966820079744016No Hit
GTTGAAATTCTTCCATGTCCGGGATAGGGTACTGTAGGCACCATCAATAG332220.10682568952361923No Hit
TTAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAA330760.10635622499197007No Hit
GTTGAAATTCTTCCATGTCCGGGATAGGGTATCTGTAGGCACCATCAATA324210.10425006562053034No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGAT318240.10233040585755399No Hit
CCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGATCG314750.10120819269628305No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)