FastQCFastQC Report
Sat 16 Sept 2023
SRR15906296_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15906296_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28773862
Total Bases4.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGA12085104.200027094034161No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA10380883.607746502711384No Hit
ACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG9050483.1453824307630307No Hit
ACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG7920862.7527969655237796No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6966932.4212703876872697Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG5549311.9285940830605222No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGAT4844761.6837364410797548No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA3542641.2312007334990347No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAG3492121.2136431320898111No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA2891881.0050371409997032No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAG2882321.001714681192257No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG2786530.9684240509668115No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAATA2678320.93081700329278No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAAT2658130.9238002184065524No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGCTGTAGGCACCATCAAT2654310.922472624634121No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGATC2397660.8332770901591173No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCA2211220.7684821731611836No Hit
TCGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA2063680.7172064702332972No Hit
AGTGAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAAT2012580.6994472969947517No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGA1868970.6495374169793405No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATA1666540.5791853731695801No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATAGA1482070.5150751053160677No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGA1453750.5052328394429639No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTCTGTAGGCACCATCAATAGA1357790.4718831278192687No Hit
CCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA1329800.46215554936629644No Hit
GTGAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAATA1320140.45879833579517404No Hit
TGCAGCGCACCACGGCCCTGTGCGTGCTGTAGGCACCATCAATAGATCGG1318410.4581970956835756No Hit
TAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGG1252570.43531521767915615No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAAT1245530.4328685527163507No Hit
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATA1206330.4192450773552747No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1192320.4143760750642371Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGTCTGTAGGCACCATCAA1154380.40119049712548144No Hit
CTAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCG1140440.39634582246901723No Hit
TGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGA1091330.3792782491276284No Hit
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAAT1081380.3758202496418451No Hit
TTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAAT1073480.3730747023114242No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGA1071830.37250126521076665No Hit
TACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCG951430.3306577337446047No Hit
AACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAATAGATC931160.3236131458474361No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATA870900.30267052785614945No Hit
TAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAAT858880.2984931254622685No Hit
TTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAA814790.28317019105742564No Hit
TGTGAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAAT797740.2772446743506311No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAA792190.27531584046660124No Hit
TAGTGAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAA770290.26770476622151035No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGAT764830.265807210724789No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTCTGTAGGCACCATCAATAGATCG747650.25983651412521547No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGCTGTAGGCACCATCAATA725630.2521837353637131No Hit
ACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAATAGATCG716090.24886822630900224No Hit
TCCAGTTGGTACGTCTGGTTCTGTAGGCACCATCAATAGATCGGAAGAGC713120.24783603952781866No Hit
TTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATA678580.2358320895540543No Hit
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATAG662950.23040007629146203No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAAT647510.22503409517985454No Hit
ACTGCGATCTGTTGAGACTATCCTTTGACTGTAGGCACCATCAATAGATC645810.22444328119735893No Hit
TACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATC624580.21706505716889862No Hit
TCTCCAGTTGGTACGTCTGGTTCTGTAGGCACCATCAATAGATCGGAAGA614250.2134749933811457No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC603930.2098884049697604No Hit
AAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGATCG599280.20827235495881644No Hit
TCTAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATC585050.20332689438769114No Hit
TGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATAG579830.20151274792379278No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAATAG575910.2001504003876852No Hit
CAAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGATC574880.1997924366218202No Hit
ACGGTGGCCATGGAAGTCGGTATCCGCTCTGTAGGCACCATCAATAGATC563520.19584440906820225No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAATA559700.19451681529577086No Hit
TCTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG540900.1879831077246426No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAAT534160.18564070405286576No Hit
TAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGA516790.17960397530230734No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTCTGTAGGCACCATCAATAGATCGG513600.17849533024103612No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATCTGTAGGCACCATCAATAGAT510520.17742491431980872No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGAT505230.175586440221337No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCCTGTAGGCACCATCAATAG501910.17443261526728668No Hit
AGTGAAACGGGAAGAGCCCAGCGCCGAATCGACTGTAGGCACCATCAATA498400.17321275816225154No Hit
CTAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGG491280.17073829018850512No Hit
AAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAATAGA480910.16713432489528168No Hit
ACCTGAACTCAGTCGTGATTACCCGCTGAACTGTAGGCACCATCAATAGA465870.1619073588383791No Hit
TTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGATCGGA464770.16152506743794073No Hit
ACAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG462510.16073963237885827Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAG439790.15284357727162243No Hit
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA432070.1501605867158187No Hit
GTGAAACGGGAAGAGCCCAGCGCCGAATCGACTGTAGGCACCATCAATAG427290.14849935681209564No Hit
TGCGGTCCGGATCGTATGGGTTATCAATTCTGTAGGCACCATCAATAGAT416710.1448224086151522No Hit
CCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGAT416600.14478417947510833No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGAT414310.14398831828692304No Hit
TTCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT412790.14346006107904458Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGCTGTAGGCACCATCAAT410080.14251823408341918No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTCTGTAGGCACCATCAATAG409050.14216027031755418No Hit
TTAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAA407410.14159030859326427No Hit
AGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAATA395340.1373955293175452No Hit
ACTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA391700.136130492319731No Hit
TCAAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGAT375710.13057336550790435No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGCTGTAGGCACCATCAATAG375410.13046910421687571No Hit
CTCCAGTTGGTACGTCTGGTTCTGTAGGCACCATCAATAGATCGGAAGAG368610.12810584828689314No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGCTGTAGGCACCATCAATAGAT368130.1279390302212473No Hit
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAAT331750.1152956109958406No Hit
TCGGCCTGGGAATCCTGGATGTTGTAAGCTTCTGTAGGCACCATCAATAG317680.11040575644659725No Hit
GTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAGAT312440.10858465922996363No Hit
TCCAGTTGGTACGTCTGGTTCCTGTAGGCACCATCAATAGATCGGAAGAG302980.10529695318619377No Hit
ACCTGAACTCAGTCGTGATTACCCGCTGACTGTAGGCACCATCAATAGAT298270.10366005091704408No Hit
TCTAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCG297730.10347238059319254No Hit
TACGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAG289180.10050093379887623No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)