FastQCFastQC Report
Sat 16 Sept 2023
SRR15906297_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15906297_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30890715
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGA12885544.171331094149164No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA11586893.750929688743041No Hit
ACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG9733323.150888543693469No Hit
ACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG8520812.7583725400982138No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA7130522.3083052626007525Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG6011221.9459633744314433No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGAT5163311.6714763643379573No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA3784371.2250833300556494No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAG3772131.2211209743769285No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA3096471.0023950562491026No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAG3060680.9908090505512741No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG2948310.9544324241119055No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAAT2866850.928062040648784No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAATA2865820.9277286071235321No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGCTGTAGGCACCATCAAT2839970.9193603968053184No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGATC2555260.8271935434320636No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCA2402930.7778809911004002No Hit
TCGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA2310460.7479464298576449No Hit
AGTGAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAAT2194040.7102587298481113No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGA2013420.6517880858374434No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATA1786380.5782902726596002No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATAGA1586950.5137304202897214No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGA1546430.5006132101506877No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTCTGTAGGCACCATCAATAGA1467750.47514277348387696No Hit
CCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA1456690.4715624096107844No Hit
GTGAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAATA1426480.46178277194296086No Hit
TGCAGCGCACCACGGCCCTGTGCGTGCTGTAGGCACCATCAATAGATCGG1412370.45721505636887977No Hit
TAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGG1346350.43584293856584416No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAAT1332760.4314435583637349No Hit
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATA1286700.41653292906946315No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1257220.4069896083661385Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGTCTGTAGGCACCATCAA1230470.3983300483656659No Hit
CTAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCG1229780.3981066802759341No Hit
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAAT1158440.3750123621288792No Hit
TGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGA1157460.37469511469708616No Hit
TTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAAT1150030.3722898612091044No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGA1138080.36842138487244463No Hit
TACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCG1019550.33005063171894855No Hit
AACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAATAGATC1009790.3268911062757855No Hit
TAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAAT960110.31080860381509456No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATA931670.3016019538557136No Hit
TTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAA880830.28514393402677796No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAA850900.2754549384823239No Hit
TGTGAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAAT850250.27524451926735916No Hit
TAGTGAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAA829790.26862116982400697No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGAT821710.2660054971210605No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTCTGTAGGCACCATCAATAGATCG795970.2576728962084562No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGCTGTAGGCACCATCAATA781580.25301453851100564No Hit
ACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAATAGATCG775670.25110134226417224No Hit
TCCAGTTGGTACGTCTGGTTCTGTAGGCACCATCAATAGATCGGAAGAGC761880.24663721768822766No Hit
TTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATA730260.23640113218486525No Hit
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATAG706650.22875805885360698No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAAT701940.22723332884978545No Hit
ACTGCGATCTGTTGAGACTATCCTTTGACTGTAGGCACCATCAATAGATC693480.22449464183655188No Hit
TACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATC677900.21945105511478125No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC658160.21306078541723622No Hit
TCTCCAGTTGGTACGTCTGGTTCTGTAGGCACCATCAATAGATCGGAAGA652360.21118319857601225No Hit
AAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGATCG651550.21092098386197924No Hit
TCTAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATC624930.20230350770449956No Hit
TGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATAG614140.1988105487360846No Hit
CAAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGATC610620.19767104775658315No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAATAG608570.19700741792477128No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAATA601280.19464748549847422No Hit
ACGGTGGCCATGGAAGTCGGTATCCGCTCTGTAGGCACCATCAATAGATC601100.19458921556202244No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAAT587110.19006034661224255No Hit
TCTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG580730.18799500108689618No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATCTGTAGGCACCATCAATAGAT563080.18228131009593013No Hit
TAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGA547790.17733160271622073No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTCTGTAGGCACCATCAATAGATCGG543900.17607232464512396No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCCTGTAGGCACCATCAATAG539970.1748000976992601No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGAT536990.173835406529114No Hit
AGTGAAACGGGAAGAGCCCAGCGCCGAATCGACTGTAGGCACCATCAATA535280.1732818421328221No Hit
CTAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGG525680.17017411218872724No Hit
AAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAATAGA516440.16718292211753596No Hit
ACCTGAACTCAGTCGTGATTACCCGCTGAACTGTAGGCACCATCAATAGA498520.16138182622189226No Hit
TTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGATCGGA488430.1581154725619009No Hit
ACAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG481530.15588179166458271Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAG466820.1511198429689957No Hit
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA462900.14985085324182365No Hit
GTGAAACGGGAAGAGCCCAGCGCCGAATCGACTGTAGGCACCATCAATAG459710.14881818047915046No Hit
TTAGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAA455840.14756537684543722No Hit
TGCGGTCCGGATCGTATGGGTTATCAATTCTGTAGGCACCATCAATAGAT449710.14558096178738497No Hit
AGCGGAGGAAAAGAAACTAAAAAGGATTCCCTCTGTAGGCACCATCAATA447060.14472309883406714No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGAT444590.14392350581720106No Hit
CCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGAT441590.14295234020967143No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTTCTGTAGGCACCATCAATAG440620.14263832999657017No Hit
ACTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA435090.14084814806002388No Hit
TCCTTGAAGACTGAAGTGGAGAAGGGTTCCACGCTGTAGGCACCATCAAT431880.13980900085996714No Hit
TTCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT425390.13770804592901134Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGCGATCTGTTGAGACTATCCTTTGATTGCTGTAGGCACCATCAATAG404010.13078687236601677No Hit
TCAAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGAT400970.1298027578837201No Hit
CTCCAGTTGGTACGTCTGGTTCTGTAGGCACCATCAATAGATCGGAAGAG389590.12611880301249095No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGCTGTAGGCACCATCAATAGAT387390.12540661490030258No Hit
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAAT357190.11563021445117085No Hit
TCGGCCTGGGAATCCTGGATGTTGTAAGCTTCTGTAGGCACCATCAATAG340350.11017873817423779No Hit
GTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAGAT335430.10858602657788918No Hit
TCCAGTTGGTACGTCTGGTTCCTGTAGGCACCATCAATAGATCGGAAGAG321810.10417693471970461No Hit
TACGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAG317970.10293384274206667No Hit
ACCTGAACTCAGTCGTGATTACCCGCTGACTGTAGGCACCATCAATAGAT314170.10170369963919579No Hit
TCTAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCG312490.1011598468989792No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAATA309930.10033111891388723No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)