FastQCFastQC Report
Sat 16 Sept 2023
SRR15906298_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15906298_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30932155
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG12665904.094735720805744No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGA10751873.475952451421506No Hit
ACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG10745163.4737831877539733No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA10299573.329729208973639No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC5105481.6505413218057392No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG4241161.371116884678743No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3532881.1421383346876413Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG3257371.0530692090479954No Hit
TGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGA2920450.9441469564600333No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAAT2804280.9065905689403146No Hit
TAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA2773760.8967238137788978Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA2747290.8881663757342481No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGAT2718370.8788168816559984No Hit
TGCAGCGCACCACGGCCCTGTGCGTGCTGTAGGCACCATCAATAGATCGG2660440.8600887975635709No Hit
TAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGA2486580.8038819151139002No Hit
TAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGG2267930.7331949552173135No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAG2084860.6740105886576606No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGAT1916670.619636750171464No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTCTGTAGGCACCATCAATAGA1814720.5866775205283952No Hit
CTAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCG1784650.5769562450466189No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAG1748170.5651626923504036No Hit
TCGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC1652790.5343274660300907No Hit
TTGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAA1538910.4975114084356554No Hit
ATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1502530.48575018455713803No Hit
CTAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGG1498610.48448289490337804No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGATC1405830.454488217843212No Hit
ACTGCGATCTGTTGAGACTATCCTTTGACTGTAGGCACCATCAATAGATC1374310.44429817450481546No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATA1352150.4371341085029478No Hit
TACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATC1309800.4234428542078623No Hit
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATA1286360.4158649793394608No Hit
ATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1269760.4104983956015997No Hit
TTCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1211080.39152784537643753Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA1195870.38661063220457803No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGCTGTAGGCACCATCAAT1194590.38619682333804417No Hit
TTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAAT1139440.36836748037761996No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATAGA1125480.36385437742698495No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAATA1047640.33868962573089395No Hit
ACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGGAAGAGC1031980.33362693287939366No Hit
TACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCG1026590.33188440960547366No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATCTGTAGGCACCATCAATAGAT1016690.32868385665337574No Hit
TCGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGAT1012360.3272840188470541No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC956830.30933182637937773Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCTAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATC948600.30667116468283573No Hit
TCTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG941340.3043240925179639No Hit
TTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATA931740.3012205260189599No Hit
TCTAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCG930560.300839045970124No Hit
AGTAGAGCAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT907250.2933031985647298Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA900280.2910498799711821No Hit
AGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA892830.28864138305268416Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCGACTCTTTACTTATTCGGTTAAACTGTAGGCACCATCAATAGATCGGA887070.28677924315328174No Hit
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATAG873360.2823469622468916No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGA872260.2819913452522141No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGA869300.2810344122483545No Hit
TTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGATCGGA851520.27528634846165745No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCA821960.2657299499501409No Hit
ACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCA820430.2652353190393621No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAAT818750.2646921949020364No Hit
TCGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA802200.25934177557302424No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGA800250.2587113636279141No Hit
TACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGAT786660.25431787730276145No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATA782150.2528598476245835No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTCTGTAGGCACCATCAATAGATCG782010.25281458727980644No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAAT771580.24944269159390933No Hit
TCGACTCTTTACTTATTCGGTTAACTGTAGGCACCATCAATAGATCGGAA759790.24563112398731998No Hit
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAAT750120.24250492731592738No Hit
TCTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG628180.2030831670150366No Hit
ACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGC617440.19961105199427587No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGCTGTAGGCACCATCAATA597010.1930062745385829No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAATA573330.1853508105077063No Hit
AAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGATCG557370.1801911312031121No Hit
CTAAATCACTTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC551850.1784065804661848No Hit
TTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA542230.17529654820364116No Hit
TCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG530150.17139122702572776Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTCTGTAGGCACCATCAATAGATCG528410.1708287055977833No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCCTGTAGGCACCATCAATAG521430.16857215412246576No Hit
TGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAAG520980.16842667444282494No Hit
TAAATCACTTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT519610.16798376964036293Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAA514940.16647401385386826No Hit
CAAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGATC507350.1640202565905932No Hit
TTGACGACAGTGAAATACCACCACTCTGTAGGCACCATCAATAGATCGGA495540.16020222322046426No Hit
AGTAGAGCAGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG492750.15930024920669122Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGAA483570.15633246374201862No Hit
ACGACTTGGTCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT481680.1557214490875272Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAGATCGG465490.1504874135022277No Hit
AGTAGAGCAGTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC462290.149452891335893No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAATAG453220.14652066757068816No Hit
TCAAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGAT448050.14484926769570372No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTCTGTAGGCACCATCAATAGATCGG442450.143038853904618No Hit
GTGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGG418870.1354157186914394No Hit
GCAGCGCACCACGGCCCTGTGCGTGCTGTAGGCACCATCAATAGATCGGA415580.13435210058917654No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAAT406790.13151039751352597No Hit
TTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC406630.13145867140520923No Hit
TCCAGTTGGTACGTCTGGTTCAGGGCTGTAGGCACCATCAATAGATCGGA406380.13137784936096433No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAA397440.12848765305876683No Hit
ACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG395560.12787987128604522Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG392510.12689384234625747Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGTGGCCATGGAAGTCGGTATCCGCTCTGTAGGCACCATCAATAGATC387320.12521597670773343No Hit
GTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA382830.12376441279309508No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGAT376320.12165980676095797No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGAT369810.11955520072882085No Hit
TATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT361480.11686221021458092No Hit
TGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATAG359030.11607015418098092No Hit
CAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGATC357550.11559168767905113No Hit
AAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATAGATCGGA357330.11552056428011563No Hit
GTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC352430.11393645221291565No Hit
AGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGATCG345640.11174132549122426No Hit
TCCAGTTGGTACGTCTGGTTCCTGTAGGCACCATCAATAGATCGGAAGAG340580.11010548731570755No Hit
TATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG338500.10943304790759002No Hit
CTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCGG336690.10884789630725696No Hit
CCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGAT329870.10664307094025618No Hit
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAAT324260.10482942426740069No Hit
CAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA321080.10380136786460561Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA319650.10333906577152481No Hit
ACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT318810.10306750370286195No Hit
TCTCCAGTTGGTACGTCTGGTTCCTGTAGGCACCATCAATAGATCGGAAG315020.10184224151210933No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)