FastQCFastQC Report
Sat 16 Sept 2023
SRR15906299_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15906299_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30541380
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG12643874.139914437396083No Hit
ACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG10751463.520292796199779No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGA10548003.4536749812876826No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA10422823.412687966293599No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC4907971.6069902538785084No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG4189831.3718535311763909No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3571971.1695509502190142Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG3180821.0414788067860719No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAAT2852190.9338772511261769No Hit
TAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA2780390.9103681628007642Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA2745240.8988591871094234No Hit
TGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGA2717290.8897076687431936No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGAT2661140.8713227758536123No Hit
TGCAGCGCACCACGGCCCTGTGCGTGCTGTAGGCACCATCAATAGATCGG2501480.8190461596692749No Hit
TAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGA2449570.8020495472044812No Hit
TAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGG2245830.7353400533964084No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAG2051990.6718720634103632No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGAT1832300.5999401467779124No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTCTGTAGGCACCATCAATAGA1824670.5974418968625517No Hit
CTAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCG1784080.5841517311922382No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAG1720010.5631736352450347No Hit
TCGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC1605570.5257031607609087No Hit
ATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1526090.49967945128871055No Hit
TTGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAA1520770.4979375522651563No Hit
CTAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGG1491510.48835710763560786No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATA1387590.45433114024317167No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGATC1373950.44986506831060025No Hit
ACTGCGATCTGTTGAGACTATCCTTTGACTGTAGGCACCATCAATAGATC1363360.44639764149491606No Hit
ATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1302960.42662119393426234No Hit
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATA1300410.4257862611316188No Hit
TACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATC1286800.4213300119379019No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGCTGTAGGCACCATCAAT1204570.3944058847373629No Hit
TTCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1203750.394137396542003Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA1188640.3891900104055547No Hit
TTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAAT1147200.3756215338010267No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATAGA1116000.36540588539221214No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAATA1032110.3379382333083836No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATCTGTAGGCACCATCAATAGAT1029320.337024718594903No Hit
ACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGGAAGAGC1024470.33543670914673795No Hit
TACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCG1018650.33353109780893986No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC988920.32379676360400217Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGAT963560.31549327502555546No Hit
TCTAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATC948740.3106408420313686No Hit
TTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATA945600.30961272869791734No Hit
TCTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG935720.30637777336845945No Hit
TCGACTCTTTACTTATTCGGTTAAACTGTAGGCACCATCAATAGATCGGA932460.3053103690795897No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA931580.3050222354065206No Hit
TCTAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCG921180.3016170192702491No Hit
AGTAGAGCAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT905870.2966041482081032Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA899780.29461013222061344Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATAG882790.2890471877826084No Hit
TTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGATCGGA863770.2828195713487734No Hit
TCGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA857650.280815732622429No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGA848850.2779343958917377No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGA844540.276523195742956No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCA841550.2755441961037779No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAAT820040.26850129234500864No Hit
ACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCA809900.2651812066121439No Hit
TCGACTCTTTACTTATTCGGTTAACTGTAGGCACCATCAATAGATCGGAA809780.2651419156567254No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATA792420.25945782410617985No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGA776600.2542779664835053No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAAT761420.2493076606230629No Hit
TACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGAT760690.24906864064426687No Hit
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAAT752000.24622332062270927No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTCTGTAGGCACCATCAATAGATCG747630.24479247499621823No Hit
ACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGC631940.20691271972648256No Hit
TCTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG620320.20310804554345613No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGCTGTAGGCACCATCAATA596180.19520401501176438No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAATA573350.18772891074339143No Hit
AAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGATCG551710.1806434417829188No Hit
TCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG545760.1786952652434173Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTAAATCACTTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC542180.17752308507343154No Hit
TTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA535830.17544393868253497No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCCTGTAGGCACCATCAATAG529630.17341390598591158No Hit
TAAATCACTTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT523320.17134785658015453Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTCTGTAGGCACCATCAATAGATCG521300.17068645883060948No Hit
TTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAA514130.1683388242443531No Hit
TGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAAG509920.1669603665584201No Hit
AGTAGAGCAGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG494460.16189838180200108Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGATC493560.1616036996363622No Hit
ACGACTTGGTCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT491800.16102743229022395Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGACGACAGTGAAATACCACCACTCTGTAGGCACCATCAATAGATCGGA486120.1591676604004141No Hit
AAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAGATCGG474940.15550705305392226No Hit
AGTAGAGCAGTCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC463040.15161069997491927No Hit
GCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGAA452020.1480024805689854No Hit
TCAAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGAT449300.14711188557949903No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTCTGTAGGCACCATCAATAGATCGG437330.14319261277650192No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAATAG437090.14311403086566488No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAAT418970.13718109659746874No Hit
TTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC411770.13482363927235771No Hit
ACAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG411230.1346468299729744Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG410730.13448311765873056Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAA396430.12980094547135723No Hit
ACGGTGGCCATGGAAGTCGGTATCCGCTCTGTAGGCACCATCAATAGATC395750.12957829672398563No Hit
GTGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGG394830.129277066065777No Hit
GCAGCGCACCACGGCCCTGTGCGTGCTGTAGGCACCATCAATAGATCGGA393180.12873681542877238No Hit
TCCAGTTGGTACGTCTGGTTCAGGGCTGTAGGCACCATCAATAGATCGGA391630.1282293072546165No Hit
GTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA389720.12760392621420513No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGAT366350.11995201264644885No Hit
GTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC364790.11944123022600812No Hit
AAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATAGATCGGA362550.1187077990581958No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGAT361680.11842293963141154No Hit
TATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT358620.11742102026823936No Hit
TGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATAG357780.11714598358030973No Hit
CAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGATC352400.1153844390790462No Hit
AGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGATCG340950.11163542708286266No Hit
TATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG339490.11115738712527069No Hit
TCCAGTTGGTACGTCTGGTTCCTGTAGGCACCATCAATAGATCGGAAGAG338060.11068916990653337No Hit
CTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCGG331360.10849542489566615No Hit
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAAT322700.10565992761296314No Hit
ACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT318430.10426182444932088No Hit
CCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGAT318130.1041635970607746No Hit
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA314270.1028997379948123No Hit
CAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA314020.10281788183769039Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAG313830.10275567115827773No Hit
AAGTGCAGCCTAAAGTGGGTGATACTGTAGGCACCATCAATAGATCGGAA312660.10237258434294717No Hit
TCTCCAGTTGGTACGTCTGGTTCCTGTAGGCACCATCAATAGATCGGAAG308840.10112182226212436No Hit
AAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAA308720.10108253130670586No Hit
ACTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA307670.10073873544679383No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)