FastQCFastQC Report
Sat 16 Sept 2023
SRR15906300_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15906300_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38008421
Total Bases5.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA14397953.7880947487926426No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGA14205063.7373454687844045No Hit
ACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG11407593.0013322573963284No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA10769392.833422098750169Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG9697062.5512925148876873No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC7691112.0235278913586017No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG6365861.6748551590711962No Hit
TGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGA5220191.3734298512427021No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA4825321.2695397159487367No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAAT3502700.9215589355843011No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG3386760.8910551690637187No Hit
TCGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC3228490.849414396877997No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGAT3137220.8254012972546267No Hit
TAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGA3032910.79795737897136No Hit
TGCAGCGCACCACGGCCCTGTGCGTGCTGTAGGCACCATCAATAGATCGG2806900.7384942405263297No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGAT2670200.7025285265073231No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAG2504110.6588303155240256No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCA2476340.6515240398963167No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGCTGTAGGCACCATCAAT2292760.6032242170754739No Hit
TAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGG2060990.5422456249892622No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAG1984950.5222395321289458No Hit
TTCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1940030.5104210985244559Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCG1827940.4809302654272325No Hit
CCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA1703000.4480586025923045No Hit
TCGACTCTTTACTTATTCGGTTAAACTGTAGGCACCATCAATAGATCGGA1688650.4442831234688755No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAAT1684030.4430676033608447No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTCTGTAGGCACCATCAATAGA1676290.4410312125305074No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1618720.42588456910640937Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGATC1600230.42101985767838135No Hit
CTAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGG1598150.420472610530177No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTCTGTAGGCACCATCAATAGATCG1550890.4080385238839572No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATA1531250.40287124792687384No Hit
TTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAAT1498820.3943389282075148No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA1471840.3872405012562874No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAATA1351830.3556659194024398No Hit
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATA1262470.33215533999689173No Hit
TCGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGAT1178260.3099997234823304No Hit
ACTGCGATCTGTTGAGACTATCCTTTGACTGTAGGCACCATCAATAGATC1177060.30968400397375095No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATA1167420.30714772392149625No Hit
TTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGATCGGA1160800.30540600463249973No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGA1138930.29965201658863966No Hit
TCGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA1119780.29461365943089296No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAAT1102460.29005677452372985No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATAGA1057810.2783093778086704No Hit
TACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATC1040110.2736525150571238No Hit
GTGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGG1013530.26665932794208946No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAATAG989190.26025548390973674No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGA977230.25710881280756176No Hit
TACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGAT959600.252470367027349No Hit
TTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATA950020.24994987295052323No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATCTGTAGGCACCATCAATAGAT926530.24376966357008098No Hit
TCTAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATC914370.24057037254980942No Hit
TTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAA910410.2395284981714973No Hit
GCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGAA907010.2386339595638556No Hit
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAAT843970.2220481613798163No Hit
TCTAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCG841970.22152196219885062No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAA822220.2163257452868142No Hit
TACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCG804320.2116162626171711No Hit
TTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA797440.20980613743464901No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGCTGTAGGCACCATCAATA743240.19554613963047818No Hit
TTGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGG742160.1952619920727567No Hit
CAAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGATC737820.19412013985006113No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAAT722210.19001315524262374No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGA716570.18852927355230042No Hit
TCAAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGAT708320.18635870193081686No Hit
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATAG707110.1860403514263326No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGTCTGTAGGCACCATCAA704690.1854036504173641No Hit
TCGACTCTTTACTTATTCGGTTAACTGTAGGCACCATCAATAGATCGGAA686240.18054946297295538No Hit
TCTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG682390.1795365295495964No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAATA679370.17874196878633816No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAA653770.17200661926997704No Hit
GCAGCGCACCACGGCCCTGTGCGTGCTGTAGGCACCATCAATAGATCGGA639020.1681259003103549No Hit
TCTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG622190.16369793420252843No Hit
AAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGATCG612620.1611800711216075No Hit
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA609410.16033552143615754No Hit
TTGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAA595740.1567389500342569No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTCTGTAGGCACCATCAATAGATCG556600.1464412320627579No Hit
AAGTGCAGCCTAAAGTGGGTGATACTGTAGGCACCATCAATAGATCGGAA509170.133962418486156No Hit
AAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAA506490.13325731158366194No Hit
TCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAG492450.12956339333328265No Hit
ACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGC470550.12380151230170809No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGAT468250.12319638324359752No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTCTGTAGGCACCATCAATAGATCGG466130.12263861211177385No Hit
ACTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA465850.12256494422643867No Hit
AGTGAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAAT462280.12162567868841487No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAATA458490.12062853124048484No Hit
CAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGATC457270.12030754974009575No Hit
CCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGAT454440.11956297789902928No Hit
AGTGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCG448330.11795543940117902No Hit
TCCAGTTGGTACGTCTGGTTCAGGGCTGTAGGCACCATCAATAGATCGGA448030.11787650952403417No Hit
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAAT440960.11601639541932036No Hit
ACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGGAAGAGC435300.1145272517371874No Hit
TGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATAG424250.11162000126235183No Hit
TACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGA416500.1095809794361097No Hit
TTGACGACAGTGAAATACCACCACTCTGTAGGCACCATCAATAGATCGGA415460.10930735586200753No Hit
TCTTGGTCAAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC410120.10790240404882907No Hit
ACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCA408670.10752090964262895No Hit
TGTGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCG406570.10696840050261494No Hit
TCGCCGACGGCGTTGGCTGCTCGTTCTCTGTAGGCACCATCAATAGATCG401260.10557134167715097No Hit
ACAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG396400.10429267766740428Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGGA387510.1019537223080117No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)