FastQCFastQC Report
Sat 16 Sept 2023
SRR15906301_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15906301_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40112296
Total Bases6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA15996663.9879691753371582No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGA15456643.8533421273117847No Hit
ACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG12229133.0487235136078974No Hit
ACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG10558092.6321330496763387No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA9504152.3693856866233736Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC8198802.043961781694072No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG7036811.7542775412307487No Hit
TGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGA5572971.3893420610976743No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA5326611.3279244848013687No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAAT3860140.9623333453662188No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAG3703890.9233802024197268No Hit
TCGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGATC3466000.8640741980962645No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGAT3445870.8590557867841822No Hit
TAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGA3296050.8217056435762241No Hit
TGCAGCGCACCACGGCCCTGTGCGTGCTGTAGGCACCATCAATAGATCGG2935560.7318354451712263No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGAT2867660.7149079673723987No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCA2774960.6917978467251039No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAG2774820.6917629447090239No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGCTGTAGGCACCATCAAT2500860.6234646852426499No Hit
TAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGG2246510.5600552010286322No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATAG2206780.5501505074653418No Hit
CTAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCG2020220.5036410780375175No Hit
TTCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1924450.47976560603761Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA1886480.4702996806764689No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAAT1878380.46828034974612276No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTCTGTAGGCACCATCAATAGA1824850.4549353145977981No Hit
TCGACTCTTTACTTATTCGGTTAAACTGTAGGCACCATCAATAGATCGGA1807870.450702198647517No Hit
CTAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGG1757320.43810007784146787No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGATC1731550.4316756138815888No Hit
TTGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATA1713860.4272654948497588No Hit
TTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAAT1667050.41559575647327696No Hit
ACGCCGACGGCGTTGGCTGCTCGTTCTCTGTAGGCACCATCAATAGATCG1654320.4124221660111403No Hit
ACGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA1613120.4021510012790093No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAATA1532250.3819901009904793No Hit
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAATA1382000.3445327587331326No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATA1316030.3280864301559801No Hit
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1280280.3191739510498227Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGA1272670.3172767771757568No Hit
TCGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGAT1261190.3144148118571921No Hit
TCGGCCTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGA1256840.3133303563575618No Hit
ACTGCGATCTGTTGAGACTATCCTTTGACTGTAGGCACCATCAATAGATC1251310.31195172672239957No Hit
TTGGGAATCCTGGATGTTGTAAGCTCTGTAGGCACCATCAATAGATCGGA1227890.30611311803243574No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAAT1221130.30442784925599875No Hit
TGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATAGA1176060.293191893079369No Hit
TACTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATC1120090.27923856565079197No Hit
GTGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGG1085370.2705828656629379No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAATAG1070240.2668109549251431No Hit
TCTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGA1069970.26674364389413163No Hit
TTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATA1052640.2624232729036503No Hit
TACGCCGACGGCGTTGGCTGCTCGTTCTACTGTAGGCACCATCAATAGAT1037650.25868626418193563No Hit
TTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAA1007210.25109756868567185No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATCTGTAGGCACCATCAATAGAT1006830.251002834642026No Hit
TCTAAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATC1000810.24950204795058353No Hit
GCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGGAA978320.24389528836743726No Hit
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAAT933580.23274160122871052No Hit
TCTAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCG923350.2301912610537178No Hit
CTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCCTGTAGGCACCATCAA917940.22884254743233845No Hit
TACTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCG886880.221099285864863No Hit
TTGGGTTCGTGGCGGCTAGCGTTTACTGTAGGCACCATCAATAGATCGGA836260.20847971405077384No Hit
TCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGCTGTAGGCACCATCAATA822070.20494214542094522No Hit
TACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAAT821870.20489228539797372No Hit
CAAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGATC803500.20031264228804058No Hit
TTGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCGG788760.19663795859504027No Hit
TTCCAGTTGGTACGTCTGGTTCAGGGTTGTCTGTAGGCACCATCAATAGA788710.1966254935892974No Hit
ACTGCGATCTGTTGAGACTATCCTTTGATTGAGTCTGTAGGCACCATCAA781100.19472831971523147No Hit
TTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAATAG772160.19249957668840495No Hit
ACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAATA769960.19195111643571838No Hit
TCAAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGAT762920.19019604362712123No Hit
TCTGCGATCTGTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCG736330.183567153573059No Hit
TTACCCCAACGCAAGGCGGGGTGCAATTATTAGACTGTAGGCACCATCAA731350.1823256390010684No Hit
TCGACTCTTTACTTATTCGGTTAACTGTAGGCACCATCAATAGATCGGAA729340.18182454577020474No Hit
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCCTGTAGGCACCATCAA685910.17099744178194137No Hit
TCTGCGATCTGTTGAGACTATCCTTTCTGTAGGCACCATCAATAGATCGG667050.166295641615728No Hit
GCAGCGCACCACGGCCCTGTGCGTGCTGTAGGCACCATCAATAGATCGGA657070.16380762646944966No Hit
AAGGCGTGGAGCTTGCGGCTTAATTTGCTGTAGGCACCATCAATAGATCG641170.15984375464321463No Hit
TTGACGACAGTGAAATACCACCACCTGTAGGCACCATCAATAGATCGGAA639400.15940249343991678No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTTCTGTAGGCACCATCAATAGATCG604310.15065455240956538No Hit
AGTGAAACGGGAAGAGCCCAGCGCCGAATCGATCTGTAGGCACCATCAAT555400.13846128379188266No Hit
AAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAA545540.13600318465938724No Hit
AAGTGCAGCCTAAAGTGGGTGATACTGTAGGCACCATCAATAGATCGGAA540810.13482399511611104No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGCTGTAGGCACCATCAATAGAT529500.13200441081707215No Hit
ACTCCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATA513590.12803804598968854No Hit
CTCCAGTTGGTACGTCTGGTTCAGGGTTGTTTCTGTAGGCACCATCAATA507890.12661703533500052No Hit
TCCAGTTGGTACGTCTGGTTCAGGGTCTGTAGGCACCATCAATAGATCGG505310.12597384103866804No Hit
CCAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGAT505110.12592398101569652No Hit
CCTTGGTCAAGGTGTTGTATTCAGTAGAGCAGTCTGTAGGCACCATCAAT502950.12538549276760425No Hit
CAGTTGGTACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGATC500330.12473232646667745No Hit
TCGGGAGGCATCTCTATCTCGTGCTGTAGGCACCATCAATAGATCGGAAG500170.12469243844830025No Hit
TGTTGACTCTGAATAAAGCAGTTTACTGTCACTGTAGGCACCATCAATAG485070.12092800671395125No Hit
ACGTCTGGTTCAGGGTTGTTCTGTAGGCACCATCAATAGATCGGAAGAGC478580.11931004896852575No Hit
TCCAGTTGGTACGTCTGGTTCAGGGCTGTAGGCACCATCAATAGATCGGA478090.11918789191224556No Hit
AGTGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCG462320.11525642910094201No Hit
TACGCCGACGGCGTTGGCTGCTCGTTCTAGCTGTAGGCACCATCAATAGA454440.11329194419586452No Hit
TTGACGACAGTGAAATACCACCACTCTGTAGGCACCATCAATAGATCGGA450610.11233712475596012No Hit
ACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGGAAGAGC449350.1120230066112396No Hit
TCGCCGACGGCGTTGGCTGCTCGTTCTCTGTAGGCACCATCAATAGATCG433950.10818378484243336No Hit
TGTGCAGCGCACCACGGCCCTGTGCGTCTGTAGGCACCATCAATAGATCG430600.10734862945766058No Hit
GTTGAGACTATCCTTTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA421310.10503263139063394No Hit
AAATCACTTGCATACGACTTGGTCTCTGTAGGCACCATCAATAGATCGGA415250.10352187269459719No Hit
ACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGAAGAGCA414910.10343711065554562No Hit
TCTTGGTCAAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC407520.10159478280674834No Hit
AAAATCACTTGCATACGACTTGGTCCTGTAGGCACCATCAATAGATCGGA402030.10022612517618039No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)