FastQCFastQC Report
Thu 14 Sept 2023
SRR15960259.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR15960259.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72859393
Total Bases7.2 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1341090.18406549173419548Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGGGGGGGGGATGCGTGCATTTATCAGATCAAAACCAAAAAAAAAAAAA1327120.18214809997113207No Hit
CGCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1300880.17854664257222125Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1252470.17190233797308743Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCAAAAAAAAAAA1229840.168796355467853No Hit
CCGAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1144180.15703946366942695Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCAAAAAAAAAAA1063790.14600588286537058No Hit
GGCCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCAAAAAAAAAAA1022520.140341548000544No Hit
TCCAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA976200.1339840972872228Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACG909050.12476771526219001Illumina Single End PCR Primer 1 (96% over 32bp)
GGGGGGGGGGATGCGTGCATTTATCAGATCAAAACCAAAAAAAAAAAAAA803170.110235615056524No Hit
GGGGGGGGGGGATGCGTGCATTTATCAGATCAAAACCAAAAAAAAAAAAA735880.10100001793866167No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)