FastQCFastQC Report
Fri 30 Jun 2023
SRR1596102.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1596102.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87231633
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA42927234.921062293996032No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA29889443.4264450832876188No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT22848442.619283763723648No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCT20918812.3980761657872436No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA15563691.78417959915986No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT14708811.6861784531764985No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT8048230.9226274601554232No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGCTGTAGGCACCATCAATA7888180.9042797582386196No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA7207730.82627479873041No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCT6240280.7153689304429277No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT5853390.6710169004860885No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT5617760.6440049104663672No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT5430040.6224851941038407No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA5044890.5783326330713079No Hit
TGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATATCT4885770.5600915438554268No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAT4454760.5106817156569796No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTCG4080680.46779818967736164No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT4016610.4604533770449993No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT3825560.438551918430783No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATATCTC3547500.40667586722811894No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATATCTCG3464790.3971942150847962No Hit
CTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATATCTCG3218490.3689590449372878No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATATCTCGT2932830.33621175015719357No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT2826530.32402580380445245No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAA2809930.3221228244116443No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC2756100.31595189786255634No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAT2692630.30867586761788585No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTC2674710.3066215669721556No Hit
TGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATATC2650990.30390236991207076No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATATCTC2593120.29726830861919096No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATATC2556990.2931264625070128No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT2382460.27311881230057905No Hit
GGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATATCT2377830.27258804154222355No Hit
GGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATATCTC2307280.26450037912278906No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATATC2268470.260051305012254No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2052300.23527015710000523No Hit
CTCGCCCGTCACGTTGAACGCACGTTCTGTAGGCACCATCAATATCTCGT1885670.21616814166484766No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATATCTCG1879960.21551356260864682No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATATCT1859930.21321737723286688No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTCTGTAGGCACCATCAATATC1845700.2115860882714416No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATATCTC1743480.19986786215500518No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCCATATCT1668960.19132508960367622No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCTGTAGGCACCATCAATATC1658280.19010076310276114No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGCTGTAGGCACCATCAATA1647490.18886382649743585No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATATC1636620.187617718907085No Hit
TGGCCGCCGGTGAAATACCACTACTCTCCTGTAGGCACCATCAATATCTC1583660.1815465268201502No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATATCTCGT1581300.18127598276189555No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1564840.1793890525928822No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATAT1531190.17553150701649708No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1511060.17322385790943523No Hit
GGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATATC1479830.16964373463007393No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1438030.1648518949542077No Hit
GCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATATCTCG1425370.1634005865739095No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATATCTCGT1424360.16328480288796152No Hit
TTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT1422780.1631036759337063No Hit
TGACGTACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATATCT1404850.16104822891484788No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGCTGTAGGCACCATCAATA1357110.15557544360083228No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA1355640.15540692675098722No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATATCTC1340950.15372290462566487No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCTGTAGGCACCATCAAT1321260.15146569593624368No Hit
CCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTCGT1260660.1445186747793659No Hit
GACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAATA1240750.14223624588112435No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCTGTAGGCACCATCAATATCTCG1237780.14189577306204965No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAT1233540.14140971085569382No Hit
GGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATATCT1228320.14081130408277465No Hit
TGACCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATATCT1223690.14028053332441914No Hit
GCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATATCTC1174540.13464610939932764No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1174280.13461630369799452No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATATC1153700.13225706780016375No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATAT1081980.12403527972473014No Hit
CTTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCT1075040.12323969677376097No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAT1030870.11817616666651191No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATATC1030860.11817502029338371No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGCTGTAGGCACCATCAATATCTC1027200.11775544772846336No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATATCTCG1017080.11659531812272733No Hit
GGGCCGCCGGTGAAATACCACTACTCTCCTGTAGGCACCATCAATATCTC972630.11149968956788875No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA969560.11114775301753207No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA958190.10984432677077134No Hit
GGGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATATC954330.10940182674328704No Hit
TGGGCCGCCGGTGAAATACCACTACTCTCCTGTAGGCACCATCAATATCT949620.10886188499990594No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCCTGTAGGCACCATCAATATC896460.1027677654504072No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCT872430.10001303082334823No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)