FastQCFastQC Report
Thu 29 Jun 2023
SRR1596104.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1596104.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122022773
Total Bases6.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA42614373.4923292556218177No Hit
CCTCAATCCTATTTCCTATAACAACTGTGCAGCCCAACTCTTTTTCTTTA26528902.1740941750274763No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA23118351.8945930691150576No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCG20946931.71664104043923No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCG17886071.4657976999096718No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCGT16312401.3368324288122841No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT14428131.1824128927147066No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA11698670.9587284170308111No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC10968110.8988576255351941No Hit
CGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATATCT10827190.8873089615821138No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAT9399280.7702890017095416No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATCA9345290.7658644177837197No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATATCTCGT9038260.7407027211223924No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCAA8910880.7302636861071826No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAAT8447170.6922617633021665No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATATCTCGT7365540.6036201127800955No Hit
GGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATATCTCG7347550.6021457978175927No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAT6673090.5468725087898142No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT6597110.5406458022388985No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATCAATATCTCGTA6432210.5271319313485853No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCAT4283060.35100497183423296No Hit
GGGGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATATCTC4222090.3460083635371899No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATAT4059190.3326583964781722No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCT3975010.32575968421894497No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA3851170.31561075898512814No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGTCCCTGTAGGCACCATCAAT3758440.30801135784711264No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATATCTCG3676520.30129785691724936No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGTAGGCACCATC3615210.2962733849688861No Hit
GGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATATCTC3601900.2951826049716146No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT3544810.2905039701072848No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGTAGGCACCATCA3502220.2870136380198473No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGTCCGCTGTAGGCACCATCAATATC3064040.25110394762131816No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT2978800.24411836633150436No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGTAGGCACCATCAATATCTCGT2880250.23604200504441902No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGTAGGCACCATCAATATCTCG2873860.23551833230342997No Hit
GGGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATATCTCGT2872410.2353995020257407No Hit
TGGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATATCT2818430.23097573761907542No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA2680870.2197024321025715No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATATCTC2680370.21966145614474766No Hit
ACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTCG2665150.218414147988589No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATATCTCGT2647480.21696605763909332No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATCTGTAGGCACCATCAA2603480.21336017335059249No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATC2398460.19655839160449173No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGTAGGCACCATCAATA2282570.1870609841000745No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAATA2163800.17732755507859177No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAT2031290.166468106736109No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT1944540.15935877805366708No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATATCT1942500.15919159614574568No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCTGTAGGCACCATCAATATCTCGTA1876650.15379506250034164No Hit
GCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCG1840650.1508447935370228No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCCTGTAGGCACCATCAATATCTC1835800.15044732674613123No Hit
TTCCCGTGGATCGCCTCAGCTGCGGCGGGCGTCGCTGTAGGCACCATCAA1801280.14761834661797105No Hit
CCCTCCGCGCGCCGGGTTTCGCCCCCGCGGCGTCCTGTAGGCACCATCAA1792980.14693814571809477No Hit
TTACCCGTCGCCGCGCTCTCCCTCTCCCCTGTAGGCACCATCAATATCTC1780790.14593915186634876No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATATC1777210.14564576400832982No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCCTGTAGGCACCATCAA1751690.14355435112099937No Hit
TGCGCCGCGACCGGCTCCGGGACGGCCGGGCTGTAGGCACCATCAATATC1729880.141766979840722No Hit
GCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1727700.14158832466460994No Hit
CTCGCCCGTCACGTTGAACGCACGTTCCTGTAGGCACCATCAATATCTCG1725990.1414481868888523No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAT1723390.14123511190816818No Hit
CCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATATCTCGTA1608510.13182047583855516No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCTC1519070.12449069650302079No Hit
GGGGGGGGATGCGTGCATTTATCAGATCTGTAGGCACCATCAATATCTCG1517040.12432433411425586No Hit
TGGCCGCCGGTGAAATACCACTACTCTCATCTGTAGGCACCATCAATATC1510220.12376542204953825No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1497320.12270824233768232No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT1478130.1211355850764021No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCCTGTAGGCACCATCAAT1470820.12053651657301709No Hit
TTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGTAGGCACCATCAATA1448670.11872128164141951No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATATC1448440.11870243270082054No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGCTGTAGGCACCATCAATATCT1336290.10951152536092586No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACTGTAGGCACCATCAATATCT1327070.10875592869865366No Hit
CCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATATCTCGTATG1323530.10846581891726062No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1320250.10819701663393602No Hit
GGCCGCCGGTGAAATACCACTACTCTCACTGTAGGCACCATCAATATCTC1312370.10755123553863179No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATATCT1278820.10480174876864994No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)