FastQCFastQC Report
Mon 3 Jul 2023
SRR1598975.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1598975.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92575736
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCGTGTACTCCGAAGAGGATCCAAACTCGTATGCCGTCTTCTGCTTGAA17040101.840665895435063TruSeq Adapter, Index 4 (96% over 27bp)
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCTCGTATGCCGTCTTCTGCT7643040.8255986212197114TruSeq Adapter, Index 21 (96% over 25bp)
CCCCATGTGAGAGTAGGTCATCGTCAAGATTCGTATGCCGTCTTCTGCTT6324210.6831390462831427Illumina Single End Adapter 1 (95% over 21bp)
CTAAACCATTCGTAGACGACCTGCTTTCGTATGCCGTCTTCTGCTTGAAA6289870.679429650983277TruSeq Adapter, Index 16 (95% over 24bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6171100.6666001553582032No Hit
TGGATGGTTTAGTGAGGCCCTCGGATCGGTCGTATGCCGTCTTCTGCTTG3975720.4294559429697648Illumina Single End Adapter 1 (95% over 23bp)
GGATGGTTTAGTGAGGCCCTCGGATCGGTCGTATGCCGTCTTCTGCTTGA3966480.42845784126415154Illumina Single End Adapter 1 (95% over 23bp)
CGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGTCGTATGCCGTCTTCTGC3400660.36733815435180556No Hit
GAAAAAATTAGAGTGTTCAAAGCAGGCCCTCGTATGCCGTCTTCTGCTTG2949390.3185921200777707TruSeq Adapter, Index 19 (95% over 24bp)
GCTGAGAAGACGGTCGAACTTGACTATCTTCGTATGCCGTCTTCTGCTTG2831850.30589548864078164Illumina PCR Primer Index 1 (95% over 22bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTTCGTATGCCGTCTTCTGCTT2750770.2971372541936907Illumina Paired End PCR Primer 2 (95% over 21bp)
AGCGTGTACTCCGAAGAGGATCCAAATCGTATGCCGTCTTCTGCTTGAAA2201150.2377674858561211Illumina PCR Primer Index 3 (95% over 22bp)
CGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGATCGTATGCCGTCTTCTG2173320.23476129857611933No Hit
TTGAAAAAATTAGAGTGTTCAAAGCAGGCCCTCGTATGCCGTCTTCTGCT1961270.21185572858961663TruSeq Adapter, Index 19 (95% over 22bp)
TGAAAAAATTAGAGTGTTCAAAGCAGGCCCTCGTATGCCGTCTTCTGCTT1950070.21064590834038846TruSeq Adapter, Index 13 (95% over 23bp)
GGCATTAACGCGAACTCGGCCTACAATAGTGACTCGTATGCCGTCTTCTG1739850.1879380143410364No Hit
CTAAACCATTCGTAGACGACCTGCTTCTCGTATGCCGTCTTCTGCTTGAA1710660.18478492031648552Illumina PCR Primer Index 3 (96% over 26bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTTCGTATGCCGTCTTCTGCT1691440.18270878235307791TruSeq Adapter, Index 13 (95% over 22bp)
AAAAAATTAGAGTGTTCAAAGCAGGCCCTCGTATGCCGTCTTCTGCTTGA1650760.17831454237641708TruSeq Adapter, Index 13 (95% over 24bp)
AAAAATTAGAGTGTTCAAAGCAGGCCCTCGTATGCCGTCTTCTGCTTGAA1611350.17405748737444549TruSeq Adapter, Index 13 (95% over 24bp)
CCCATGTGAGAGTAGGTCATCGTCAAGATTCGTATGCCGTCTTCTGCTTG1589140.17165837061236003Illumina Single End Adapter 1 (95% over 22bp)
TGGATGGTTTAGTGAGGCCCTCGGATCGGCCTCGTATGCCGTCTTTTGCT1568640.16944396747761206No Hit
GCGGGTATAGTTTAGTGGTAGAACCTCAGCCTCGTATGCCGTCTTCTGCT1425890.15402416028320856TruSeq Adapter, Index 14 (95% over 22bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTCGTATGCCGTCTTCTGCTTG1368190.14779142560638134Illumina PCR Primer Index 1 (96% over 27bp)
AGGGAGTCGGGTTCAGATCCCCGAATCCGGATCGTATGCCGTCTTCTGCT1363610.14729669554017913Illumina Single End Adapter 1 (95% over 21bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTATCGTATGCCGTCTTCTGCT1289040.13924166911295202TruSeq Adapter, Index 19 (95% over 22bp)
CTGAGAAGACGGTCGAACTTGACTATCTTCGTATGCCGTCTTCTGCTTGA1177760.1272212407795494Illumina PCR Primer Index 1 (95% over 22bp)
GACCGCCTGGGAATACCGGGTGCTGTAGGCTTTCGTATGCCGTCTTCTGC1140550.12320182904081908TruSeq Adapter, Index 13 (95% over 21bp)
GCTGAGAAGACGGTCGAACTTGACTATCTCGTATGCCGTCTTCTGCTTGA1097450.11854618147459288RNA PCR Primer, Index 37 (96% over 28bp)
TCCCCATGTGAGAGTAGGTCATCGTCAAGATTCGTATGCCGTCTTCTGCT1072610.115862972993269No Hit
CGGCGGTAACAGGGGTTCGAGTCCCCTAGGGTCGTATGCCGTCTTCTGCT1050820.11350922449052958Illumina Single End Adapter 1 (95% over 21bp)
CCCCATGTGAGAGTAGGTCATCGTCAAGATCGTATGCCGTCTTCTGCTTG1049840.11340336521872212Illumina Single End Adapter 1 (95% over 24bp)
GCTGAGAAGACGGTCGAACTTGACTATCTATCGTATGCCGTCTTCTGCTT1007610.1088416947611413TruSeq Adapter, Index 19 (95% over 23bp)
TTCCCCATGTGAGAGTAGGTCATCGTCAAGATTCGTATGCCGTCTTCTGC998810.10789112170817633No Hit
AAAATTAGAGTGTTCAAAGCAGGCCCTCGTATGCCGTCTTCTGCTTGAAA980300.10589167770699656TruSeq Adapter, Index 19 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)