FastQCFastQC Report
Thu 29 Jun 2023
SRR1605293.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1605293.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29776606
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAA12382284.158391994037198Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA4177521.402953714738342Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG3743491.2571916356081685Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACA3541361.1893094867830134No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG2717630.9126728546564374Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTAT2239460.7520870578735535TruSeq Adapter, Index 2 (100% over 48bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA2080530.6987129426369144Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC1961330.6586815166241579No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA1762750.5919915788925038No Hit
GACCTGGTATTGCAGTACCTCCAGATCGGAAGAGCACACGTCTGAACTC1735170.5827292741153911Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1569740.5271722371582578Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG1482710.49794459449139367No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACG1326750.44556790656396505No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTG1262370.42394690650774636Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1206900.4053181883791591Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGCACACGTCTG1119250.375882328563571Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT1114060.37413934952828404No Hit
ACCCCGAGTGTTACAGCCCTCCGGAGATCGGAAGAGCACACGTCTGAAC1077960.36201573812676974Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGA1065110.357700269802408Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT1040210.3493380004423607Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT991310.3329157124220269No Hit
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTC990890.33277466209547185No Hit
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA892470.29972186890608016Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTG858000.28814566710524364Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC838360.2815498851682425No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCT790300.2654096978010187Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGAGAGCGACGCTCAGACAGGCGTAGATCGGAAGAGCACACGTCTGAA779190.2616785808295277Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCT749080.2515666157519766Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGAGAGCGACGCTCAGACAGGCAGATCGGAAGAGCACACGTCTGAACT718430.24127330025456895Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG697190.2341401837402154No Hit
GGGAGAGCGACGCTCAGACAGGAGATCGGAAGAGCACACGTCTGAACTC627300.2106687377332393Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGGAGATCGGAAGAGCACACGT620970.2085429078115887No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACAC608000.20418713939392555No Hit
AAGGGCCAGCGAGAGCTCACCGGACGCAGATCGGAAGAGCACACGTCTG567250.1905018993769807Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACG546810.18363745015130334No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC535010.17967460764332913No Hit
AGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAGATCGGAAGAGCACACG519350.17441544546749216No Hit
CGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACGTCT507580.1704626779828433Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACCTGGTATTGCAGTACCTCCAGAGATCGGAAGAGCACACGTCTGAAC501020.16825960621569833Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGA496120.1666140190725565Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGTCT475920.159830170033482Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGAAGGTGGCTCGGGGGGAGATCGGAAGAGCACACGTCTGAACTCC469310.15761030656079475Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGTCCGCCCGGAGGATTCAGATCGGAAGAGCACACGTCTGAACTCCAGT466370.15662295427490966Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACGCCCGCGGTCGGCGGGAGAGGCAGATCGGAAGAGCACACGTCTGAAC462120.15519565930381723Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGACGAAGCCGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA453410.1522705441983549Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCTGA441790.14836815183033283Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCT439910.14773678370194374Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GATACCATGATCACGAAGGTGGTAGATCGGAAGAGCACACGTCTGAACT432620.1452885530338817Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT416790.13997229905920106No Hit
CGACCTGGTATTGCAGTACCTCCAGATCGGAAGAGCACACGTCTGAACT415870.1396633316772234Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACA411100.13806140296849143No Hit
CCCGCGGACGCTACGCCGCGACGAGTAGATCGGAAGAGCACACGTCTGA389520.13081410285645045Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGCCCGAGGTGGGATCCCGAGGCCTAGATCGGAAGAGCACACGTCTGA387610.130172659704736Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTC373650.125484415517336Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACAC358280.12032264523364415No Hit
CCCGCGGACGCTACGCCGCGACGAGTAGGAAGATCGGAAGAGCACACGT353580.11874422491267138No Hit
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCAC340590.11438173981279129No Hit
GTAGTGTTTCCTACTTTATGGAAGATCGGAAGAGCACACGTCTGAACTC336360.11296116152391578Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGATACCATGATCACGAAGGTGGTAGATCGGAAGAGCACACGTCTGAAC335810.1127764527629509Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GACCTGGTATTGCAGTACCTCCAAGATCGGAAGAGCACACGTCTGAACT330320.11093272349441034Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGTACGAAACCCCGACCCAGAAGCAGATCGGAAGAGCACACGTCTGAAC326790.10974722908312652Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAAC317050.10647620484349359Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGT317010.10646277147905976No Hit
GACTGCATAATTTGTGGTAGTGGGAGATCGGAAGAGCACACGTCTGAAC315130.10583140335067066Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC309250.10385669877890046Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGTCTG306660.1029868884318112Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGGAGGTTTCTGTCCTCCCTGAGCTCGCAGATCGGAAGAGCACACGT305890.10272829616646034No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACT297890.10004162327969816Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)