FastQCFastQC Report
Thu 29 Jun 2023
SRR1605294.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1605294.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19730128
Total Bases966.7 Mbp
Sequences flagged as poor quality0
Sequence length49
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC7412493.7569396407362383No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT6615973.3532321736584785Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACT5121482.5957662312175573Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTAT4912142.489664537401886TruSeq Adapter, Index 6 (100% over 48bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCT4779732.4225539743077187Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG4534112.298064158529534Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG4195782.1265852912865038Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG2195231.1126283620663788No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA2124031.0765414192954044Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC2073241.0507990622260535No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG1954900.9908197250418244No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1758080.8910636565561054Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC1603680.8128077020078126No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA1315050.6665187372327235No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC1310570.6642480981370217No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1272120.6447601353625279No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGA1144680.5801685625151545Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCT1051390.5328855443816685Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT1031880.522997113855521Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACA943520.4782128124054745No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGT806020.40852243837444946No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT775560.3930841198800129No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACG769290.38990623882419817No Hit
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT767040.3887658508855087No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC737020.37355054158797146No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC718770.36430072830748994No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA716040.3629170576085467Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTC689600.3495162322312354Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA665560.3373318206551929Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCA644240.32652601138725507No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACA620020.3142503687761174No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCA616100.3122635595673784No Hit
CGACGAAGCCGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA607190.307747623330168Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGA597150.30265895892819344Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACAC595340.30174158018640324No Hit
AATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT578060.29298340081726787Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCA566300.28702297319105075No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAA558580.2831101754636361Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAG520610.26386549544939597Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAAC500370.2536070723920291Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCAC478400.24247181771958093No Hit
AGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAGATCGGAAGAGCACACG473650.24006433207123642No Hit
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAA470990.2387161401081635Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACG456950.23160011937074101No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACAC447330.2267243273839886No Hit
GAACGGAAGGACGGACGGCGCCGGACGCGCAGATCGGAAGAGCACACGT434530.220236787110555No Hit
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC427680.21676493938610025Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG387450.196374803042332Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA377140.19114929208771478No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA360310.18261919030631732No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACA349200.1769882080846105No Hit
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGCACACGTCTG341110.17288787989616694Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCGGCGCTCCCCCGGGGAGGGGGGAGGACAGATCGGAAGAGCACACG336550.1705766936737562No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG335910.1702523166600845No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA322590.16350122006304266No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA322140.16327314247530478No Hit
AATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC319180.16177289878707327Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCAGATCGGAAGAGCACA314040.15916774589602256No Hit
GTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAACT311160.1577080493345Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACAGATCGGAAGAG308420.15631931024471812No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCAGATCGGAAGAGCACAC308340.15627876311800917No Hit
CGACCTGGTATTGCAGTACCTCCAGATCGGAAGAGCACACGTCTGAACT308070.15614191656536644Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACGAAGCCGAGCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGT302020.15307554010800134Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA299780.15194022056015047No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACAGATCGGAAGAGCACACGTC296730.15039436135437134No Hit
GTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTC273500.13862048943625707Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA268590.13613190953449464No Hit
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACAC267170.1354121980354106No Hit
AGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACA267170.1354121980354106No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACA257360.1304401066227244No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACAC253890.12868137500172325No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC248150.12577211866035537No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG246350.12485980830940378No Hit
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACAC232970.1180783013673302No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT224190.11362825421102185No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA218390.11068858752462224No Hit
AATCCCCGATCCCCATCACGAATGGGGTTCAGATCGGAAGAGCACACGT218310.11064804039791329No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAGATCGGAAGAGCACACG218140.11056187775365675No Hit
TGGAAAGGATGAACGAACTTGGCCTGACCAGATCGGAAGAGCACACGTC212920.1079161777358971No Hit
GACGGCGCGACCCGCCCGGGGCGCACTGGGGACAGATCGGAAGAGCACA210130.10650209669192212No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC208470.1056607438127112No Hit
GGACGGCGCCGGACGCGCACGCCCCGCCGGGCCAGATCGGAAGAGCACA206550.10468761277169615No Hit
GGGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC201640.10219903286993375Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGCTCCCCGGACCCCGTCCCGGCCCGGAGCGGACAGATCGGAAGAGCA198240.10047577998480293No Hit
GTATAGTGGTTAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCC198130.10042002768557812Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)