FastQCFastQC Report
Thu 29 Jun 2023
SRR1605295.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1605295.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28869948
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC8306602.87724799504315No Hit
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAA7302362.529398390326162Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACT6896392.388778116261242Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT6511932.2556084964198755Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGA6328632.192116868378149Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCT5876942.035660057302493Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG4577581.5855865067716783Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACCTGGTATTGCAGTACCTCCAGATCGGAAGAGCACACGTCTGAACT3929691.36116975340586Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC3346751.1592504427094914No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG3073581.0646295587369954No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG3006591.0414254989305836Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTAT2807240.9723744566495236TruSeq Adapter, Index 2 (100% over 48bp)
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACA2399310.8310752759236005No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG2379930.8243624131224622No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC2256140.7814839153849532No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC2188290.757981967961979No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCA2037250.7056645893508364No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA1954630.677046595303878No Hit
CGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCT1781730.6171573291368588Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1758870.6092390606314912Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGT1632800.5655708143291426No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGA1467560.5083348262352256Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACCTGGTATTGCAGTACCTCCAGATCGGAAGAGCACACGTCTGAACTC1417020.4908287330479431Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT1407250.4874445911714147Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACAC1394210.4829277835900502No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC1303670.4515664524231218No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA1268030.43922143538325736No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG1255500.43488128208613336No Hit
GGGGGCCCGAGGTGGGATCCCGAGGCCTAGATCGGAAGAGCACACGTCT1224940.42429588026968396Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCAC1218670.42212407171637445No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1217820.421829647909307Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1211610.41967862221296687No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT1190450.41234920132173425No Hit
CGACCTGGTATTGCAGTACCTCCAGGAAGATCGGAAGAGCACACGTCTG1144520.39643992431160596Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCT1140670.395106357656065Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA1066670.3694741673937203Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACCAAGATGAGTGGTGCAAATCTGATCAGATCGGAAGAGCACACGTCT982020.3401530200192948Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA812220.2813375347956983No Hit
GGGGCCCGAGGTGGGATCCCGAGGCCTAGATCGGAAGAGCACACGTCTG807200.2795987024292527Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACA798530.2765955795971645No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCA791520.2741674491412316No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTC771080.26708742253363255Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACAC743920.257679715945453No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT706030.24455534176923352No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG698980.24211335607532097Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCAC608150.21065157443304017No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTG597520.20696954493994932Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA566450.19620748883925943No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCAGATCGGAAGAGCACAC551250.190942498406994No Hit
CGCGAGCCGGGCCCTTCCCGTGGATCGCCTCAGCAGATCGGAAGAGCAC545380.188909242233481No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGT543530.18826843747692237No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA532120.1843162308432284Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACCTGGTATTGCAGTACCTCCAAGATCGGAAGAGCACACGTCTGAAC526320.18230722133617974Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGGGAGACCGGGGTTCGATTCCCCGACAGATCGGAAGAGCACACGTCTG514550.17823031756066895Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTC505240.1750055109209064No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA504530.1747595804467677Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGT499980.173183547126583No Hit
GGGGGGCCCGAGGTGGGATCCCGAGGCCTAGATCGGAAGAGCACACGTC492120.17046099286358257No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGA487540.1688745681149131Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA460290.1594356872412794No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC456150.15800167011038604No Hit
ACCCCGAGTGTTACAGCCCTCCGGAGATCGGAAGAGCACACGTCTGAAC443540.15363380633730273Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAATACAAGCTTGGGCTGCAGGTCGAGATCGGAAGAGCACACGTCTGAA443230.15352642824296045Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGCACACGTCTG442300.1532042939599337Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCT440240.15249074920398192Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCAGATCGGAAGAGCACACGTC437590.15157283968783042No Hit
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCC430280.14904079494705014Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAACT426420.1477037644820143Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACG425340.14732967305656386No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACG422980.14651221401576478No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC421910.14614158639980923No Hit
GTATAGTGGTTAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCC420730.14573285687940968Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA413810.14333590070893096Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGACGTACGCAGTTTTATCCGGTAAAGATCGGAAGAGCACACGTCTGA403690.13983052550008057Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGACGTACGCAGTTTTATCCGGTAAAAGATCGGAAGAGCACACGTCTG379020.131285307476134Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTC363890.12604456371033299No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACAC362500.1255630941905403No Hit
GGGAGAGCGACGCTCAGACAGGCAGATCGGAAGAGCACACGTCTGAACT361000.12504352276630357Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGGCCCGAGGTGGGATCCCGAGGCCTAGATCGGAAGAGCACACGTC356740.12356793992147129No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGCGCAGATCGGAAGAGCACA356510.12348827230308833No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACAGATCGGAAGAGCACACGTC349340.12100472089523681No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACAC347770.1204609028045357No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA341130.1181609332999145No Hit
GTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAACTCCA339220.11749934568638641Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAA328490.11378267809834641Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTC326730.11317304762724201No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACG303790.10522706864591512No Hit
GGGAGACCGGGGTTCGATTCCCCGACAGATCGGAAGAGCACACGTCTGA292540.10133028296413972Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG290210.10052321535182537Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCAAGATGAGTGGTGCAAATCTGATCAGATCGGAAGAGCACACGTCTG289090.10013526868839527Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)