FastQCFastQC Report
Thu 29 Jun 2023
SRR1605296.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1605296.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29399992
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC303330310.317359950302027No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACT18080116.149698952299035Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGT8352272.8409089363017515No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC6207432.1113713228221287No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT6000922.0411298071101514Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCA5842961.9874019013338506No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGA4610591.5682283178852567Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCT4514551.5355616423296987Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC4370851.486684078009273No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG3235541.1005241089861522No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCA2931880.9972383665954739No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACG2466180.8388369629488335No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG2383790.8108131457994954Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT2072600.7049661782220893Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC2062480.701524000414694No Hit
CGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCT1992040.6775648102217171Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA1866990.6350307850423905No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACAC1654900.5628913096302883No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGC1533520.5216055841103631No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGT1437410.48891509902451674No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG1375180.4677484265982113No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1330020.4523878782007832Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACAGATCGGAAGA1278890.4349967170059094No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTAT1221800.4155783443750597TruSeq Adapter, Index 6 (100% over 48bp)
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACA1184960.4030477287204704No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACGT1076760.3662449976176864No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACG1027090.34935043519739734No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACAGATCGGAAGAG946550.3219558699199646No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTC916460.3117211732574621Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC892160.3034558648859496No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACA887860.3019932794539536No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCAC859950.29250007959185836No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCAC848610.28864293568515254No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA843710.28697626856497105No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCAGATCGGAAGA802990.27312592466011554No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAA794450.270221161964942Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACAC782440.2661361268397624No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCAC765460.26036061506411295No Hit
GTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAACT736230.2504184354880097Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG687590.23387421329910565Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCAGATCGGAAG681410.23177217191079508No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGC631660.2148503985987479No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAAGATCGGAAGAG621330.21133679220048768No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGAGATCGGAA559590.1903367864862004No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA554580.18863270438985155Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACA533800.18156467525569395No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGT509390.17326195190801413No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA492870.16764290275997354No Hit
GTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGC490680.1668980045980965No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA478880.1628843980637818No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAGATCGGAAGAGC464490.1579898389087997No Hit
GTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTC459280.1562177295830557Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACAC455880.15506126668333786No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC413270.14056806546069808No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCA393520.13385037655792562No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGC380790.12952044340692337No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC371740.12644221127679217No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAGATCGGAAGAGCA366330.12460207472165298No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC365420.124292550827905No Hit
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCC362200.12319731243464285Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAAGATCGGAAGAGC357630.12164289024296333No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATGTCGGAGATCGGAAGA354570.12060207363321732No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACAC352220.11980275368782413No Hit
CGCGAGCCGGGCCCTTCCCGTGGATCGCCTCAGCAGATCGGAAGAGCAC346200.11775513408302969No Hit
GTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTC345190.11741159657458411Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAAC345090.11737758295988653Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGCACACGTCTG341920.11629935137397317Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGA335340.11406125552687225Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACA334380.11373472482577546No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG327870.11152043850896287Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGAGATCGGAAGAGCACAC326710.1111258805784709No Hit
TGGAAAGGATGAACGAACTTGGCCTGACCAGATCGGAAGAGCACACGTC319540.10868710440465426No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACG294190.10006465307881716No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)