FastQCFastQC Report
Thu 29 Jun 2023
SRR1605297.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1605297.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23188207
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC7246803.1252092928099184No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACT5430372.3418671396197213Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT4200831.8116234687744508Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCT4034871.7400526051884908Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG2928971.2631291414640209Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG2759131.1898850135329566Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG1444280.6228510897802491No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1358750.5859659610594299Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1294570.5582880987736568Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC1265880.5459154302012225No Hit
ACGGGGTCGGCGGCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1232580.5315546820847339Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG1070460.4616398326959907No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC1063400.4585951815938162No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC1060920.45752567242478037No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTC1020800.44022377409344327Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC996470.4297313716407655No Hit
CGACGAAGCCGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA923220.3981420383214623Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGT897330.3869768801011652No Hit
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAG885920.3820562754162062Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGT877900.3785976207647275Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGCACACGTCTG832320.35894107724672286Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAA822200.3545767898311413Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACA774180.3338679872919885No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA742520.3202144952388945No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAAC723980.31221905169295755Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT714630.30818682962421373Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACCCGGCGGCGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC708380.3054914940167647Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACAC689720.29744430002716465No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGA686820.2961936643053083Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACAGATCGGAAGAG669840.2888709765269906No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCA614820.26514339810749493No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACAGATCGGAAGAGC599730.2586357798168698No Hit
CGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCT563380.242959707923946Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT560060.24152794564926908No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCA526270.2269558832211563No Hit
CGACGAAGCCGAGCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGT448740.19352078407787202Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC443820.19139901588768807No Hit
GTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTC427940.18455070717628147Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGCGAGCCGGGCTTCTTACCCAGATCGGAAGAGCACACGTCTGAACTCC426780.1840504528875389Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAA426150.18377876305830804Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCAGATCGGAAGAGCA424980.18327419623259356No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGT414240.17864253152475307No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACAC413960.17852178048953934No Hit
AGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAGATCGGAAGAGCA400500.17271710572533702No Hit
AGCTGGGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGATCGGAAGA393340.16962932925344335No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT387340.16704180707029223No Hit
GGGGGTGGGGGCCGGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC364190.15705828398030083Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC359270.15493651579011694Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAGATCGGAAGAGCACACG352590.15205574109287537No Hit
ATCCGAGTCACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG336010.14490555479343445Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CCGACGAAGCCGAGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTC334910.1444311757265234Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCAC334030.14405167247299458No Hit
AGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAACAGATCGGAAGAGCACA326740.14090783302046597No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG302860.13060949473152453Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACAC291360.1256500772138182No Hit
CCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGTCTG287030.1237827487049775Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA280200.12083728595315714No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA279970.12073809760280302No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA272720.11761150829816208No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC271180.11694737760448662No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTAT266330.11485579717310614TruSeq Adapter, Index 9 (100% over 48bp)
AAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGTCTGAAC263750.11374316263435115Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACAGATCGGAAGA255180.11004731844941698No Hit
GAACGGAAGGACGGACGGCGCCGGACGCGCAGATCGGAAGAGCACACGT255170.11004300591244506No Hit
ACGCCCGGCTCCACGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTC252120.10872768213600992Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA249350.10753310939478847Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA246980.1065110381324438No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCGGAAGAGCAC246730.10640322470814582No Hit
ACGCCCGCGGTCGGCGGGAGAGGCAGATCGGAAGAGCACACGTCTGAAC244670.1055148420919306Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGTGAGATCGGAAGAGCACAC244610.1054889668700991No Hit
GTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTC242970.10478171080670447Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAAGATCGGAAGAGC234370.10107292901085452No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA232510.10027079713407767No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)