FastQCFastQC Report
Thu 29 Jun 2023
SRR1605300.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1605300.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25606128
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACT7132702.7855441478696035Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC5871232.2929003557273475No Hit
CGACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGA5026561.9630300996698915Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAA4993861.9502597190797455Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACCTGGTATTGCAGTACCTCCAGATCGGAAGAGCACACGTCTGAACT3749951.4644736603675494Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCT2946071.150533184868872Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT2926241.1427889448963156Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG2404650.93909161119557Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGCCCGAGGTGGGATCCCGAGGCCTAGATCGGAAGAGCACACGTCT2404520.9390408420984226Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC2147330.8386000413650982No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCT2074540.810173252277736Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCA2062000.8052759870605974No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG1993570.7785519153852547No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC1811200.7073306827178244No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGT1612820.6298570404709373No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC1603500.6262172867369874No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTAT1564980.6111740127207049TruSeq Adapter, Index 12 (100% over 48bp)
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACA1501780.5864924208767527No Hit
GACCTGGTATTGCAGTACCTCCAGATCGGAAGAGCACACGTCTGAACTC1420550.5547695457899765Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGCCCGAGGTGGGATCCCGAGGCCAGATCGGAAGAGCACACGTCTG1327940.5186024220452229Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCCCGAGGTGGGATCCCGAGGCCTAGATCGGAAGAGCACACGTCTG1300470.507874521286467Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1278680.49936483954153477Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACG1276640.49856815524783754No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1210810.4728594655154422No Hit
GGGGCCCGAGGTGGGATCCCGAGGCCAGATCGGAAGAGCACACGTCTGA1038950.40574271908661863Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC1022340.39925599059725075No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGA952190.3718602047134967Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CACCAAGATGAGTGGTGCAAATCTGATCAGATCGGAAGAGCACACGTCT931870.3639246042978462Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACAC901210.35195090800139717No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT891230.3480534034665452Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTC873300.3410511733753733No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACAC862290.3367514213785075No Hit
CGACCTGGTATTGCAGTACCTCCAGGAAGATCGGAAGAGCACACGTCTG846830.3307138041331356Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGAGAGCGACGCTCAGACAGGCAGATCGGAAGAGCACACGTCTGAACT832500.3251174875014294Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA816790.31898223737692794Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCAC785920.30692652946201004No Hit
GGGAGAGCGACGCTCAGACAGGAGATCGGAAGAGCACACGTCTGAACTC776580.3032789650977297Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA758230.2961127117696201No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA707230.27619560442719027No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT704910.27528957130886794No Hit
GGGGGCCCGAGGTGGGATCCCGAGGCAGATCGGAAGAGCACACGTCTGA673310.26294877538689176Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTC668510.2610742241076042Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGACGTACGCAGTTTTATCCGGTAAAGATCGGAAGAGCACACGTCTGA624650.24394551179311455Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGACGTACGCAGTTTTATCCGGTAAAAGATCGGAAGAGCACACGTCTG604150.2359396157044907Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGT590590.2306440083405035No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC589600.23025738213915042No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCTAGATCGGAAGAGCACACGTC582280.22739869143823696No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG582080.22732058513493333No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTG576950.22531715845519482Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACGTCTG573260.22387609715924253Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGT559730.21859220574075078No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCAGATCGGAAGAGCACAC555330.21687386706807057No Hit
AGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAGATCGGAAGAGCACACG542850.212000033741923No Hit
CGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA542300.211785241407838Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC536440.20949672672104114No Hit
CGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCT518780.20259994013932917Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGA497300.1942113231645175Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCGACAGATCGGAAGAGCACACGTCTG494070.1929499063661636Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACAC489840.19129795805129146No Hit
AGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTCTG489470.19115346139017975Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGATCGGAAGAGCACA484530.18922423569857966No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA478950.1870450698364079No Hit
CGCGAGCCGGGCCCTTCCCGTGGATCGCCTCAGCAGATCGGAAGAGCAC477520.18648660976778683No Hit
GAATACAAGCTTGGGCTGCAGGTCGAGATCGGAAGAGCACACGTCTGAA470100.18358886591522153Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGAGACCGGGGTTCGATTCCCCGACAGATCGGAAGAGCACACGTCTG451870.17646947636909416Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTC446690.1744465231135297Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACG444310.1735170581042163No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACAGATCGGAAGAGCACACGTC418940.16360927353014873No Hit
CGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACGTCTG412140.16095365921782473Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGATCGGAAGAGCACACGT388210.15160824002754342No Hit
GGGGGGCCCGAGGTGGGATCCCGAGGCCTAGATCGGAAGAGCACACGTC387060.15115912878354745No Hit
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCC383360.14971416217242997Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GTGCATTTATCAGATCAAAACCAAGATCGGAAGAGCACACGTCTGAACT377860.14756623883157968Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCCGGCTAGCTCAGTCGGTAGAGCAAGATCGGAAGAGCACACGTCTGA372220.14536364107841684Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTCTAGGGGTATGATTCTCGCAGATCGGAAGAGCACACGTCTGAACTCC367210.1434070781806605Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGGAGATCGGAAGAGCACACGT365150.14260258325663294No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCAC363130.1418137095932661No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAA354630.13849419170286112Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGTAGTGTTTCCTACTTTATGGAAGATCGGAAGAGCACACGTCTGAACT353600.13809194424084736Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG348920.136264256743542Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CCCTGGTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAA347930.13587763054218896Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCCGCCGCCCTCTCCCACGTCCGGGAGATCGGAAGAGCACACGTCTGA338130.13205042168031028Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACCCCGAGTGTTACAGCCCTCCGGAGATCGGAAGAGCACACGTCTGAAC332090.12969161132054013Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGATACCATGATCACGAAGGTGGTAGATCGGAAGAGCACACGTCTGAAC332020.1296642741143839Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCCCGGGGAGCCCGGCGGGTGCCGGCGCGGGAGATCGGAAGAGCACACG322690.12602061506526874No Hit
GGGGCCCGAGGTGGGATCCCGAGGCAGATCGGAAGAGCACACGTCTGAA318730.12447411025985654Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGGGCCCGAGGTGGGATCCCGAGGCCAGATCGGAAGAGCACACGTCT310050.12108429669647827Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACA303390.11848335679646685No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACA301730.11783507447904658No Hit
TGTCATAAGATTCGGTGCAATAAGTAGATCGGAAGAGCACACGTCTGAA301100.11758903962364009Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGAGACCGGGGTTCGATTCCCCGACAGATCGGAAGAGCACACGTCTGA300570.11738205791988543Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTC299400.1169251360455591No Hit
GGGAGAGCGACGCTCAGACAGGCGTAGATCGGAAGAGCACACGTCTGAA298240.11647211948639793Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGACCTGGTATTGCAGTACCTCCAAGATCGGAAGAGCACACGTCTGAAC296410.11575744681116959Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGATCGGAAGAGCACACGTCT291190.11371887229494439Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACAC289160.11292609331641239No Hit
AAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGTCTGAAC287000.112082545240733Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGC280290.10946207876489567No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA278470.10875131140483246No Hit
CGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTCTGAACT277740.10846622339777416Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGAGATCGGAAGAGCAC276390.10793900585047454No Hit
GTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGC276220.10787261549266645No Hit
TCCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGT272590.10645498608770525No Hit
GGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACGTC267470.1044554647231319No Hit
GGGAGACCGGGGTTCGATTCCCCGACGAGATCGGAAGAGCACACGTCTG263890.10305736189399663Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCACACGTC257100.10040565289683781No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)