FastQCFastQC Report
Wed 9 Aug 2023
SRR1605302_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1605302_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29904842
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT6996632.339631154045221Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG6042102.0204420407905848TruSeq Adapter, Index 9 (100% over 49bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC5987632.002227599129265Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG2893800.967669382770857Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT2523590.8438733767595228Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC2451480.8197602247823279Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA2416480.8080564344730529No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT2209070.7386997731002892Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT2203370.7367937272499215No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC2077610.6947403366986523No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA2006310.670898043868615No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC1819210.6083329248153192Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC1694600.5666640873742118No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG1374400.4595911257447874Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGAGATCGGAAG1347580.4506226784277944No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1235700.4132106767191748No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCGGAAGAGCACA992050.33173557646617896No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTC975320.32614116469834553No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACAGATCGGAAGAGC929040.31066540996939557No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA865570.28944142222854746Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG837100.27992122479697434No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG820000.2742030872458714No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT800290.2676121813317054No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT797580.26670597356775866No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG779400.2606266904871124Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCT778420.2602989843584527Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA777520.25999802975049996No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG749110.25049789595945704No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA735010.24578294043486335Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC693400.23186880572717955Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC638370.2134671034209109No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC636570.21286519420500535No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGT612040.20466250916824774No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC573590.19180505952848706No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC559470.18708341612371668No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA545620.18245205910133214No Hit
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA534820.17884060380589872Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC520080.17391163611564978No Hit
GTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC517910.1731860011164747Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA506520.1693772533558278No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG500910.16750130296625543No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACAGATCGGAAGAG493720.1650970100427215No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC493560.16504350700130768No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC481770.1611010016371262No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCAC477140.15955275737621352No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT470790.1574293554201022Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG452880.15144035872184178No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACG448840.15008940692614262No Hit
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC448430.14995230538251966Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG443770.1483940293013419No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC442520.14797603679029636No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCAC437500.1462973788659375No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA427250.1428698402753641Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAAGATCGGAAGAGCA426650.14266920387006224No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGAGATCGGAAGA419290.14020806396502614No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC418360.13989707753680825No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC405520.1356034584633485No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC402290.13452336581480684No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG397430.1328982109318618Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA393760.1316709849194321Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC387090.12944057688049315No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG381710.12764153711295315No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC380860.12735730220544217No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGT375770.12565523670046475No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA366710.12262562698040672Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT365430.1221976026490961No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA362030.12106066301905223No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA360450.12053232048509067No Hit
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGTC351920.11767993958971593No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCAGATCGGAAGAG349070.11672691666453211No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT348010.1163724590151655No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCT345000.11536593304856786Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCGTAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT343400.11483090263442956Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA334630.11189826717693407Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTC326190.10907598174235464No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTG321830.10761802386382781Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA315390.1054645264469212No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACA312580.10452487928209085No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCT305080.10201692421581764Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGGGGGATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC300330.1004285526738446Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)