FastQCFastQC Report
Thu 29 Jun 2023
SRR1605304.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1605304.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24521162
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCA6161912.5128947804349564No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC5482872.235974787817967No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC4813691.9630758118232734No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTAT3844391.5677845935686083TruSeq Adapter, Index 1 (100% over 48bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC3410831.3909740492722165No Hit
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA2876661.1731336386097853Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC2818631.1494683653246123No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC2679401.0926888375028885No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG2650071.0807277403901168No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACAC2441300.9955890344837656No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCAC2387750.9737507545523333No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGT2345310.9564432550137715No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA2286370.9324068737036197No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA2101010.8568150236925967No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCT2085080.850318594200389Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTC1910940.7793023837940469No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTG1853300.7557961568052933Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG1769270.7215277970921606No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCA1756570.7163485971831188No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCAGATCGGAAGAGCACAC1549250.6318012172506344No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACA1509530.6156029636768436No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA1446190.5897722138942681Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACT1357760.5537094857087115Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACG1212810.49459727887283644No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT1180360.4813638113887099No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGT1087690.44357196449336295No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACAC1080780.44075399036962437No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGA1009940.41186465796359895Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACAC947870.38655182817192757No Hit
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGGAGATCGGAAGAGCACACGT947690.3864784221889648No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACAC915820.373481485094385No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGC856260.3491922609540282No Hit
GGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG843830.34412317001943055Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGAGATCGGAAGAGCACACG840620.342814096656594No Hit
GGGGGCCCGAGGTGGGATCCCGAGGCCTAGATCGGAAGAGCACACGTCT787160.32101251971664313Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACAC773540.3154581336724581No Hit
CGACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGA768300.31332120394620777Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGC766760.3126931749808594No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCT752150.306735056030379Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC747640.3048958283461444No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA747020.3046429855159392No Hit
TCCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGT689570.281214242620313No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT669470.2730172411894673Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGAGATCGGAAGAGCACACGTC661830.269901565023713No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACG653700.2665860614598933No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG652790.2662149534349147No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACA647120.2639026649715866No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCAC625480.2550776345753925No Hit
CGACCTGGTATTGCAGTACCTCCAGATCGGAAGAGCACACGTCTGAACT580300.23665273285173025Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCT552320.22524218061117984Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACAGATCGGAAGAG541800.2209520087180208No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACAC536220.21867642324617406No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC535710.21846843962777945No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACG527470.21510807685214917No Hit
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAA524760.21400290899754262Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG523090.21332186460005445No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGC515090.21005937646837455No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGAGATCGGAAGAGCACACG510400.20814674280117718No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA508210.20725363667512983No Hit
TCCCGGGGAGCCCGGCGGGTGCCGGCGCGGGAGATCGGAAGAGCACACG507880.20711905903969804No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTCT486050.19821654455037652Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACAGATCGGAAGA471910.19245009677763233No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGATCGGAAGAGCACACGTCT470100.19171195883783973Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGAGATCGGAAGAGCAC451910.1842938764484326No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGAGATCGGAAGAGCACACGT439590.17926964472564555No Hit
CCTCCCCTCTTCCCCGCGGGGCCCCGTCGTAGATCGGAAGAGCACACGT432130.17622737454285406No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT429820.1752853310948315No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA428610.1747918797649149No Hit
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA422070.17212479571726658Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGAGATCGGAAGAGCACACG419280.17098700298134323No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG416640.16991038189788887No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCAGATCGGAAGAGCACACGT413350.1685686836537355No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCT402910.16431113664189323Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCAC396570.16172561479753692No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCGGAAGAGCAC395510.16129333512008934No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACA381830.1557144804149167No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCAGATCGGAAGAGCACA373890.1524764609442244No Hit
GGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCTGAACTCCA371540.15151810505554347Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCA360720.1471055898574464No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACG357430.14576389161329303No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAAGATCGGAAGAGC356290.14529898705452865No Hit
CCCGGGGAGCCCGGCGGGTGCCGGCGCGGGTAGATCGGAAGAGCACACG353950.14434470927601228No Hit
GGGGCCCGAGGTGGGATCCCGAGGCCTAGATCGGAAGAGCACACGTCTG349560.14255441891375292Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGCGGCGGCGACTCTGGACGCGAGCCGGGAGATCGGAAGAGCACACGT346280.14121679877976417No Hit
GGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAACT342820.13980577266281263Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGT337710.1377218583687021No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCAGATCGGAAGAGC336030.13703673586104934No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTAGATCGGAAGAGCACAC330620.13483047826200079No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGT330460.1347652284993672No Hit
GTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCA330360.1347244473977212No Hit
TCCCGGGGAGCCCGGCGGGTGCCGGCGCGGGTAGATCGGAAGAGCACAC322670.1315883806811439No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA318330.12981848086970757No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAAGATCGGAAGAG316550.12909257726040876No Hit
GCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAG313930.12802411239728362Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGTC307940.1255813244086883No Hit
GGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAAC301470.12294278713219219Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACAC299620.1221883367517412No Hit
CGGCGGGCGTCGCGGCCGCTCCCGGGGAGCCCGGCGGAGATCGGAAGAG291440.11885244263709852No Hit
GGCGGGCGTGGGGGTGGGGGCCGGGCCGCAGATCGGAAGAGCACACGTC285980.116625794487227No Hit
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACA282460.11519029970928783No Hit
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAG281290.11471316082002966Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCAGATCGGAAGAGCA271850.11086342482464738No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCTAGATCGGAAGAGCACACGTC267840.10922810264864284No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC267310.10901196280991904No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGAGATCGGAAGAGCACACGTC263640.10751529637951088No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCAGATCGGAAGA260780.10634895687243534No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGAGATCGGAAGAGCACAC260450.10621437923700353No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTC256800.10472586902692457Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG253780.10349427975721542Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGAGATCGGAAGAGCACACGTCT253630.10343310810474642Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGCCCGAGGTGGGATCCCGAGGCCTAGATCGGAAGAGCACACGTC253150.10323735881684562No Hit
GTATAGTGGTGAGTATCCCCGAGATCGGAAGAGCACACGTCTGAACTCC253080.10320881204569343Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CGCGAGCCGGGCCCTTCCCGTGGATCGCCTCAGCAGATCGGAAGAGCAC251050.10238095568227965No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACAC247260.1008353519298963No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)