FastQCFastQC Report
Wed 9 Aug 2023
SRR1605307_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1605307_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34667242
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT10824773.1224779865672616Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC7084012.0434305099898054Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCACA4624231.3338903625503293No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC4402001.269786618733616No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG4133731.1924023260921652Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT3170860.9146559740748917Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA2687000.775083290444622No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGT2608550.7524538583138515No Hit
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT2439730.7037565895781384Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG2367310.6828665516570369Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC2330690.6723032654284987Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC2000320.577005808538216No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG1880520.5424486897457836TruSeq Adapter, Index 8 (100% over 49bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC1878780.5419467749987149No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC1870330.5395093154511685Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACAGATCGGAAGAGC1766500.5095588509752232No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA1528400.4408772985171419Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT1497190.4318745633125358No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACACG1398850.4035077264006176No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTC1264730.36481990693116No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG1165830.336291534238576No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGGGGAGATCGGAAGAGCACA1159140.33436175857312217No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACACG1148780.331373346630805No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTC1115710.32183408186898743No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG1106460.31916585692048993Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGAA1081160.31186790111541035Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCAC989620.2854625701115768No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACAGATCGGAAGAG978710.2823155069561057No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC950920.27429929384056567No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC929700.2681782415803369No Hit
CGACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCT893240.2576611084319889Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAAGATCGGAAGAGCA840540.24245943764433295No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACAC811590.23410861469741379No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA791190.22822409697315985Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACG786730.22693757986285726No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACAC783410.22597990344891006No Hit
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACACG767490.2213876719699825No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA759830.21917809325587537No Hit
TAAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTC750260.21641756214699745No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA681990.19672461974333003No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC681320.19653135372003344No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA652400.18818918447564995Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT640830.18485173986439418No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACCAGATCGGAAGAG639630.18450559176296746No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGT626550.18073257745741642No Hit
GTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAACTCC614640.17729705755075642Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA586930.1693039209753115Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG566320.16335882733330792No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGT561690.16202327257530322No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACA559660.1614377053703897No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACAC552620.15940696984201974No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAAGATCGGAAGAGC550730.15886178658227268No Hit
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA537030.1549099290909845Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAATACAAGCTTGGGCTGCAGGTCGACCCGAGATCGGAAGAGCACACGTC530880.15313592007117266No Hit
CTCGACTGCGTAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT528700.15250708435358082Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCA519000.14970905386704833No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA514000.14826677011110373No Hit
GTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAACTC486240.14025921069809938Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTC472670.13634485258446577No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCAGATCGGAAGAGCACACGTCT455540.1314035884365996Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGT449760.12973630841472766No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACAC448570.12939304488081285No Hit
GTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACGTCTGAAC446530.12880459310838746Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACAC428330.12355468023674916No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTTAGATCGGAAGAGCACACGT396000.11422887347081144No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAGATCGGAAGAGCACACGTCTG387990.1119183348937882Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTC380630.10979529320503778No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAGATCGGAAGAGCACACG376660.10865011990281778No Hit
ATAAACGATGCCGACTGGCGATGCGGCGGCGAGATCGGAAGAGCACACGT374530.10803570702278538No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCT370960.10700591642104093Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCACAC367010.10586651225384472No Hit
GGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC365010.10528959875146686No Hit
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA359070.10357616564940471Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGT353680.10202138376049644No Hit
GTCTAGGGGGATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC352160.10158292949868927Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGT350430.10108389931913246No Hit
CGCGTCTCTCGGGGTCGGGGGTGCGTGGCGGAGATCGGAAGAGCACACGT348420.10050410124924274No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)