FastQCFastQC Report
Thu 29 Jun 2023
SRR1605310.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1605310.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29061342
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC63

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACAC7510292.5842887778547876No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCA6368492.1913957036120353No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTAT4763151.6389986394984786TruSeq Adapter, Index 8 (100% over 48bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTC4327831.4892051440707728No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTC3548441.2210172537799526No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGT3530111.2147099056884572No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCA2568650.8838717771533056No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCA2317140.7973272535039847No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACAC2288480.7874653551787113No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGCAC2186400.7523396545142341No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCAC2170410.7468374997961209No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACT2156720.7421267744621015Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGA2144490.7379184347371157Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTG1961630.6749963577043345Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTG1941190.667962959177866Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCAC1897410.6528982729015061No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGC1877980.6462124151045743No Hit
GGGGGCCCGAGGTGGGATCCCGAGGCCTAGATCGGAAGAGCACACGTCT1733170.5963833328825626Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCAGATCGGAAGAGC1722230.5926188818121337No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACA1625520.5593409967096495No Hit
GGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCT1607360.5530921455726305Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGT1590430.5472665371062355No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACG1553080.5344144121080162No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCAC1427250.4911163428034397No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACG1309980.4507637672066211No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCTAGATCGGAAGAGCACACGTC1302490.4481864602123329No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACG1238720.42624322028900113No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAGATCGGAAGAGCACA1145530.3941765662439126No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTC1074470.3697248392727356No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGAGATCGGAAGAGCACACG1073230.36929815560478935No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGAGATCGGAAGAGCACACGTCT1040330.3579772744149255Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGACCGGGGTTCGATTCCCCGACGGGGAGCAGATCGGAAGAGCACACGT1023390.3521482249512084No Hit
GGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCTGAA959740.3302462769957423Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACG845840.2910533174964873No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACGT818970.2818073576918781No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACG799930.2752556987905101No Hit
CGGTGGGGGGCGGGGCGGACTGTAGATCGGAAGAGCACACGTCTGAACT797850.2745399713475035Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGAGATCGGAAGAGC797490.2744160954439062No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACAC795390.2736934860062553No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACG786350.2705828244270344No Hit
GTATAGTGGTGAGTATCCCCGCAGATCGGAAGAGCACACGTCTGAACTC783770.2696950471179204Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTCTGA779750.2683117661944173Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCAC777010.267368932928149No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTAGATCGGAAGAGCACA772770.26590995006355866No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACAC748570.25758273654396274No Hit
GGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT744330.2561237536793724Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTATGGGAGATCGGAAGAGCACAC741780.2552462993622249No Hit
CGACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGA740790.25490564062733234Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCAGATCGGAAGAGCACAC729060.25086935076845385No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCAGATCGGAAGAGC707750.24353658547495846No Hit
TTCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACA705800.24286559099713978No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATCGGAAGAGCACACGTCT681750.2345899924373761Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAGCA665080.22885384990135693No Hit
AGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCACACGTC653540.22488293899159922No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAGATCGGAAGAGCAC647290.2227323156652573No Hit
CGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTC641360.22069180425322407No Hit
GGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTCTG582640.20048626797757652Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGATCGGAAGAGCACACGTCT558940.19233110432408798Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGT538650.1853493207574516No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC533200.18347397721688144No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACAGATCGGAAGA520350.17905229565792247No Hit
CGACCTGGTATTGCAGTACCTCCAGATCGGAAGAGCACACGTCTGAACT517900.17820925131399645Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAAGATCGGAAGAGCACACGTC514960.1771975981012852No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACG514700.1771081321709094No Hit
CTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACACGTCTGA509640.175366987525903Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAGATCGGAAGAGCACA499440.17185717025731295No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCACA488890.1682269180824478No Hit
CTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGAGATCGGAAGAGCA487870.1678759363555888No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCT486570.16742860670370968Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAGATCGGAAGAG481710.1657562820051462No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGAAGATCGGAAGAG475850.16373985757436804No Hit
GGGGGGGGGGATGCGTGCATTTATCAGAAGATCGGAAGAGCACACGTCT473420.1629036952250863Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGGCCCGAGGTGGGATCCCGAGGCCTAGATCGGAAGAGCACACGTC473300.16286240325722054No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGAGATCGGAAGAGCACACGTC461240.15871256048671117No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGAGATCGGAAGAGCACACGT446540.15365429442315498No Hit
GGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCAC444840.15306932487839No Hit
GGGGCCCGAGGTGGGATCCCGAGGCCTAGATCGGAAGAGCACACGTCTG441790.15201982069513514Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGAGATCGGAAGAGCACACGTC437320.1504816948921354No Hit
TTGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG434560.1495319796312228Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGCGGGCGTGGGGGTGGGGGCCGGGCCGCAGATCGGAAGAGCACACGTC434270.14943219070888053No Hit
GGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACAC433730.1492463768534846No Hit
CTCGGTTGGCCCCGGATAGCCGGGTCCCCGAGATCGGAAGAGCACACGT433370.14912250094988733No Hit
CGCGGGAGACCGGGGTTCAATTCCCCGACGGGAGATCGGAAGAGCACAC431370.1484343014854579No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGAGATCGGAAGAGCACACGTC428850.14756717016027682No Hit
GGGCGGGCGTCCCGCGCGTGTGGGGGGAAGATCGGAAGAGCACACGTCT427700.14717145546822993Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGCAGCGGAAGCGTGCTGGGCAGATCGGAAGAGCACACGTCTGAACTC421470.14502771413653232Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGACAGATCGGAAGAGCACACG415570.14299752571646554No Hit
GTGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGAGATCGGAAGAGCA415560.1429940847191434No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAAGATCGGAAGAGCACACG415220.1428770908101904No Hit
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCAGATCGGAAGAGCACACGT399730.1375469859581846No Hit
TCTTGCGACCCGGGTTCGATTCCCGGGCGGCGAGATCGGAAGAGCACAC386850.13311498140725916No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAAGATCGGAAGAGCAC386450.13297734151437327No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTAGATCGGAAGAGCACAC370740.12757153472128024No Hit
GGGGGCCCGAGGTGGGATCCCGAGGCCTCAGATCGGAAGAGCACACGTC363310.12501487371092498No Hit
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGT359320.12364191577938831No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCA358210.12325996507662998No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCAGATCGGAAGAGCACACGTCT346710.11930281815616085Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGGCCCGAGGTGGGATCCCGAGGCCAGATCGGAAGAGCACACGTCTG346640.11927873117490582Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCAGATCGGAAGAGCACA346420.11920302923381859No Hit
GGGGGGGGGGATGCGTGCATTTATCAGATAGATCGGAAGAGCACACGTC345250.11880043254712737No Hit
GTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTGAAC344690.11860773669708714Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT344000.11837030788185901Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAGATCGGAAGAGCACACG343870.11832557491667109No Hit
CGGGAGACCGGGGTTCAATTCCCCGACGGGAGATCGGAAGAGCACACGT343190.11809158709876509No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGC325170.11189090992425607No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAA325090.11186338194567891Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCGACAGATCGGAAGAG324720.11173606504475947No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGAGATCGGAAGAGCACACG321690.1106934428561489No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCAGATCGGAAGAGCACACGTCT318060.1094443608282095Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGAGATCGGAAGAGCAC316380.1088662732780888No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGAGATCGGAAGAGCACA315050.10840862063424325No Hit
GGGGGGGGATGCGTGCATTTATCAGATCAGATCGGAAGAGCACACGTCT313700.10794408599575339Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGTGGTTCAGTGGTAGAATTCTCGCAGATCGGAAGAGCACACGTCTG313540.10788903003859905Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGC309430.1064747801391966No Hit
CGGTTGGCCCCGGATAGCCGGGTCCCCGTCCGAGATCGGAAGAGCACAC306280.10539086598272028No Hit
CTAGCTCAGTCGGTAGAGCATGAGAAGATCGGAAGAGCACACGTCTGAA303390.10439641775661977Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGACCGGGGTTCGATTCCCCGACGGGGAGATCGGAAGAGCACACGTCTG302900.10422780888783456Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGAGATCGGAAGAGCACACGT301630.10379080222792189No Hit
CTCCCCGCGTCCGGCGCTCCGGCGGCGGGCGCGGGGGAGATCGGAAGAG300700.1034707894769622No Hit
CGCCGCCGCCCTCTCCCACGTCCGGGGAGATCGGAAGAGCACACGTCTG300030.10324024265637836Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGGCTGGACGAGGCGCCGCCGCCCTCTCCCAGATCGGAAGAGCACAC299980.10322303766976762No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCAGATCGGAAGAGCACACGTCTG299960.10321615567512334Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGACCAAGGAGTCTAACGCGTGCGCGAGTCAGATCGGAAGAGCACAC299830.10317142270993543No Hit
CCAGGCGGCCCGGGTTCGACTCCCGGTGTGGGAAGATCGGAAGAGCACA294110.10120317224166729No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)