FastQCFastQC Report
Thu 29 Jun 2023
SRR1610244.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1610244.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47835548
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG21263554.445135655182627No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT13039272.725853584869562No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC4044000.8453963985109985No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG3998370.8358574673378886No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA3782250.7906776776133096No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG3315190.693038992675489No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA3201850.6693453161652919No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA2900590.6063670473682041No Hit
CCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAG2721010.5688259283660762No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG2672920.5587727352888274No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAATA2474960.517389285474476No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG2456660.5135636786266147No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG2364940.49438965348531183No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA2250090.4703803121477776No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA2185090.45679209110346136No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC2104210.4398841631332414No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA2102570.4395413218638156No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA1913770.4000727659689401No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1885690.3942026544777955No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA1602900.33508553095283866No Hit
GTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1594090.3332438043774475No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG1572880.32880986332590983No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG1561230.3263744360156593No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1546050.3232010637779252Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1512710.3162313516299636No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG1414230.2956441515000518No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT1344400.2810462211073656No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA1340550.280241380322433No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1306020.27302289920458317No Hit
TTCTAGAGCTAATACATGCTGTAGGCACCATCAATAGATCGGAAGAGCAC1215620.2541248194752572No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1211130.2531861869754267No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA1208840.2527074634955577No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG1196440.2501152490194113No Hit
ACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATA1158260.24213373702753443No Hit
CGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGG1137900.2378774880973455No Hit
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1104980.23099557676228566No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA1092100.22830301849996576No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA1046790.2188309831843047No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA999450.20893457727295192No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA987270.20638835369880154No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG980300.2049312783037418No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATCTGTAGGCACCATCAAT978600.20457589406104432No Hit
CGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGAT935170.1954968719078958No Hit
TTCTAGAGCTAATACACTGTAGGCACCATCAATAGATCGGAAGAGCACAC882100.18440261204909789No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATTAAT828760.17325190881057745No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCA824890.17244288703455432No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATTAATAGATCGG801840.16762429480268523No Hit
TATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCTGTAGGCACCA790260.16520350096125166No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC785480.1642042440906081No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA764890.15989991376287777No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATTGG743150.15535517644744032No Hit
CGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGGAA740580.1548179190923035No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAATAGAT727620.15210863686562137No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAATAGATCGG726870.1519518496997254No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA726010.15177206708283136No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA723580.15126407666532846No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAAT712010.14884537331944017No Hit
GCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAA709970.1484189122282032No Hit
ATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCG708500.1481116093830471No Hit
ACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAG706840.14776458712253074No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC700760.14649356583100082No Hit
AGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC698910.14610682415512413No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACCA683780.14294390439511637No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCAC677150.1415579058485961No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCATC677140.14155581535305084No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC644830.13480142424625302No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAAT641760.13415964211385223No Hit
TGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC626120.13089010708103524No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCATCATCAATAGATCGG599470.12531893645286557No Hit
CGGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCG581290.12151841555154758No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT579420.1211274928845803No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGG579280.12109822594694639No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA569750.11910598369229511No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACTATCAATAGATCGG560560.11718481828618332No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAG549530.11487900169974011No Hit
GGCGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGG530100.1108171688552622No Hit
ATTGATCATCGACACTTCGAACGCACTCTGTAGGCACCATCAATAGATCG523050.1093433694958402No Hit
TATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCAT511220.10687031326577463No Hit
AATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC501900.10492197141757423Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAG500440.10461675906796343No Hit
CCTAAGCGCAGACCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG490200.10247609162959731No Hit
CACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCA490120.10245936766523507No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA484580.10130123313315026No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)