FastQCFastQC Report
Thu 29 Jun 2023
SRR1610245.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1610245.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50248400
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA30812736.1320818175305085Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT12141212.416238128975251No Hit
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG7789261.5501508505743467No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG6974671.3880382260927713No Hit
TCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG3510400.6986093089531209No Hit
TAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACAC3494760.6954967720365225No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA3119140.6207441430970936No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG3040740.6051416562517413No Hit
ACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCAC3030730.6031495530205937No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC2925030.5821140573630205No Hit
TCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACA2805980.5584217606928777No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA2736890.5446720691604111No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2551870.5078509962506269No Hit
CCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA2485220.4945868923189594Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG2405390.47869981929772887No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA2088440.4156231840217798No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG2073560.4126618957021517No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA1999220.3978673947827195No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC1924320.38296144752867756No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG1918070.381717626829909No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1845490.36727338581924995No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1833100.36480763566601127No Hit
CTGTAGGCACCATTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1826580.36351008191305595Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG1790140.35625810971095595No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA1725060.34330645353881917No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA1583320.3150985902038672No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG1540060.306489360855271No Hit
TTCTAGAGCTAATACATGCTGTAGGCACCATCAATAGATCGGAAGAGCAC1531320.30475000199011315No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA1487360.2960014647232549No Hit
ATCGGGGATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1405690.27974821088830687Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTGTAGGCACTATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1338090.2662950462104266Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCATC1324750.2636402353109751No Hit
AGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC1306460.26000031841809884No Hit
TTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1304060.2595226912697718No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA1277410.2542190398102228No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT1272060.25315432929207693No Hit
TATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCTGTAGGCACCA1225250.24383860978658026No Hit
ACGTGGCGACCCGCTGAACTGTAGGCACCATCAATAGATCGGAAGAGCAC1193360.23749213905318375No Hit
CTGTAGGCACCATCAATAGATTGGAAGAGCACACGTCTGAACTCCAGTCA1174220.23368306254527507Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1106270.22016024390826375Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1056870.2103290851051974Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGTCAGCCCTCGACACACTGTAGGCACCATCAATAGATCGGAAGAGCAC1054990.2099549438390078No Hit
CTGTAGGCATCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1031250.2052304152968055Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1023980.20378360305999796Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCTCGGGCCGATCGCACTGTAGGCACCATCAATAGATCGGAAGAGCACA1009880.20097754356357614No Hit
TATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCAT991490.19731772553951968No Hit
ACGTGGCGACCCGCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACA983970.1958211604747614No Hit
CTGTAGGTACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA976820.1943982295953702Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG955550.19016525899332118No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA933640.18580492115171826No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA933050.1856875044777545No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA930350.1851501739358865No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT909480.18099680785855868No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAAT891300.17737878220998082No Hit
TTATACCCACACCCACCCAAGAACAGGGTTTGTTCTGTAGGCACCATCAA890830.17728524689343342No Hit
ACGTAGCAGAGCAGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACA880850.17529911400163986No Hit
ATTGATCATCGACACTTCGAACGCACTCTGTAGGCACCATCAATAGATCG854930.17014074079970706No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAATAGATCGG854600.17007506706681208No Hit
AATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG847050.1685725316626997Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCTAGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGCA834270.16602916709785784No Hit
ACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATA830480.16527491422612461No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA808480.16089666536645944No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT805680.16033943369341114No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC799830.15917521751936378No Hit
ATCCTGCCGACTACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC789710.15716122304391783No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAATA788070.15683484449256097No Hit
TTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACA777340.1546994531169152No Hit
TCCCACGGCGCGACCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCA771000.15343772140008438No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA767610.15276307305307235No Hit
TTCTAGAGCTAATACACTGTAGGCACCATCAATAGATCGGAAGAGCACAC760920.15143168737711052No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAAT759920.15123267606530755No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAATAGAT717280.14274683373002922No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA693980.1381098701650202No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC685880.13649787853941617Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCA676260.1345833897198717No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC675560.1344440818016096No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG674380.1342092484536821No Hit
TCAGCCCTCGACACACTGTAGGCACCATCAATAGATCGGAAGAGCACACG662700.1318847963318235No Hit
TCGGGCCGATCGCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT653200.12999418886969535No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGTAGGCACCATCA650330.12942302640482087No Hit
CCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAG628240.12502686652709338No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA625470.12447560519339919No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA624200.12422286082740942No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATC619500.1232875076619355No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGCTGTAGGCACCATCAATA616850.12276012768565765No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCAA610760.1215481487967776No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA601820.11976898766925911No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAG581760.11577682075449168No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA577110.11485141815460792No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCA547750.10900844604007291No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAA547210.10890097993169932No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG537590.10698649111215483No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA527860.105050111048312No Hit
TCCGGGACGGCTGGGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC525950.10466999944276834No Hit
TTTTATCCGGTAAAGCGAATGCTGTAGGCACCATCAATAGATCGGAAGAG524430.10436750224882782No Hit
TCCCCTTGTAAATTTAACTGTTAATCCAACTGTAGGCACCATCAATAGAT514740.10243908263745713No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG503580.10021811639773605No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)