FastQCFastQC Report
Thu 29 Jun 2023
SRR1610246.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1610246.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47265605
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG15611153.3028562736052995No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT13089672.76938589911205No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC7676491.6241175797919016No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA6830861.4452073553274098No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG5572101.1788910773489516No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA4939191.0449860950684964No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG4046290.8560749407523716No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG3933070.8321209471453925No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA3192640.6754679221814679No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG3035530.6422281064634632No Hit
CCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAG2376350.5027651714179898No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA2336440.49432139925004664No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA2224730.4706868768526289No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1977270.4183316811453064No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC1912380.40460288194766575No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG1902080.4024237074718498No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA1831030.3873916349954687No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA1815210.38404459225688536No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG1701280.3599403837103111No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCAC1692310.3580425977833141No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1661920.35161297522796964No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAATAGAT1498230.3169810266894923No Hit
CGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGG1428970.30232766511716075No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1389800.29404045499893633No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA1376680.29126465217148917No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA1344950.28455152536395123No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA1335090.2824654418366167No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG1311560.2774871917962332No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACCA1296900.27438557064910096No Hit
ACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATA1282220.27127971809521956No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAATA1250940.26466179793953765No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAATAGATCGG1224300.2590255641496602No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT1212900.2566136623026406No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA1131110.23930932440196206No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG1104890.2337619501538169No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAA1086130.22979289062310745No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA1056050.22342885487237496No Hit
ATTGATCATCGACACTTCGAACGCACTCTGTAGGCACCATCAATAGATCG1046440.2213956639294049No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCACC1023480.21653800898137238No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA1005840.21280590822861573No Hit
CCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAATAGATCGGA996830.2108996594881204No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAG943260.19956583651050275No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA930520.19687043041128957Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGCTGTAGGCACCATCAATA922610.19519690904199788No Hit
TATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCTGTAGGCACCA914690.1935212719693316No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCATC914670.19351704056258245No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA903930.191244775138285No Hit
GTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGATC870540.184180441570567No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG846850.17916834027619027No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAAT827390.17505118150926027No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGG817890.17304126330341058No Hit
GCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGGA794850.16816668272838145No Hit
TATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCAT790840.16731828567517543No Hit
CGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGAT784110.16589441730408402No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA775610.1640960694356922No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA771680.1632645980094828No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGAT755920.1599302494911469No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC745140.15764952125335113No Hit
TCAACTTCTTAGAGGGACAAGTGGCGTCTGTAGGCACCATCAATAGATCG743610.15732581863704062No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATAGATC741870.15695768624986395No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC735680.15564806586099977No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAATAGA719080.15213599825919927No Hit
TCCCACGGCGCGACCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCA699350.14796171550115564No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT680610.14399688737719532No Hit
CCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATC654570.13848759578979258No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGAT637710.13492051990025303No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATTAAT628220.13291271739777794No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATCTGTAGGCACCATCAATAG620530.13128574150272698No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA620270.13123073321498793No Hit
TTCTAGAGCTAATACATGCTGTAGGCACCATCAATAGATCGGAAGAGCAC611360.1293456415082384No Hit
TGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA610790.12922504641588742No Hit
AAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCA606130.12823912864333376No Hit
AGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC586390.12406273018191558No Hit
TAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAAT572910.12121076203298362No Hit
TAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGA565390.11961975309530049No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCA564690.11947165385907997No Hit
CTAAACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATAGATCG561980.11889829824456917No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT556200.11767542169406273No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA554560.11732844634063185No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCTGTAGGCACCATCAATAGATCG544940.1152931396942872No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC533680.11291085769451169No Hit
ACCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCA521520.11033816239102408No Hit
TAAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATA519490.10990867460598462No Hit
AGGGCACCACCAGGAGTGGAGCCTGCGGCTTAATTTGCTGTAGGCACCAT496880.10512506927606237No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCACC496830.10511449075918948No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT483170.10222443994951508No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATTGG481670.1019070844433283No Hit
AAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCG479360.10141835696380064No Hit
ACGCGCGCTACACTGACTGGCTCAGCGTCTGTAGGCACCATCAATAGATC479350.10141624126042606No Hit
ACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAG473630.10020605893016707No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)