FastQCFastQC Report
Fri 30 Jun 2023
SRR1610247.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1610247.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51940871
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG26383725.0795682652298995No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG20596653.9654032755823443No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT18368893.536500186914463No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA12091962.3280241103388506Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT10238511.971185658399914No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA8050451.5499258762911388No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA4794470.9230630730085368No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC3693020.7110046344813894No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG3548470.6831749124884717No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG3526680.6789797575785743No Hit
GTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG3368290.6484854672537163No Hit
CCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA3332500.6415949397537057Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC3252710.6262332412561968No Hit
TCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACA3229590.6217820259502387No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA3016700.5807950351852975No Hit
GTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2783690.5359344089551367No Hit
TCCGGGACGGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2212920.42604599372236174No Hit
TGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2118790.40792346358612275No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT2093140.4029851559478085No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG2077210.3999182069934869No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG2062210.3970303077897943No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA2045170.3937496542943995No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2030000.39082902556639837No Hit
TCCGGGACGGCTGGGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1967350.3787672332256423No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG1904240.36661687864263964No Hit
CCGTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1875120.36101050365520443Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TTCTAGAGCTAATACACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1830970.3525104536656692No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA1821910.35076616254663884No Hit
AGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC1800400.34662491508854365No Hit
TTCTAGAGCTAATACATGCTGTAGGCACCATCAATAGATCGGAAGAGCAC1785930.3438390549900482No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA1545680.29758453607757174No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAATA1504590.28967361752558984No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA1478970.28474108568568285No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA1468600.2827445847028634No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA1412070.2718610552372139No Hit
TTCTGATCGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1393230.26823385383737597No Hit
TAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1365830.26295862462529745No Hit
TGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1323140.25473966349158833No Hit
TTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1321900.2545009304907498No Hit
TCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1301940.2506580992837028No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA1244550.23960899693037493No Hit
AAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCA1215340.23398529454771752No Hit
ATCGGGGATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA1203350.23167690045089923Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1200930.23121098604603685No Hit
CCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAATAGATCGGA1122190.21605144049278652No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAAT1064760.20499463707491544No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA1061920.20444786149234964No Hit
CTGTAGGCACCATTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1054740.2030655204068488Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA1044570.2011075247467452No Hit
TAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGA1036640.1995807887010597No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC1018520.19609220646299905Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA963510.18550131744999038No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA922900.1776828116725266No Hit
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATTGGAAGAGCACACG909860.17517226463144986No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG901030.17347225463354282No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT898130.17291392745416226No Hit
TCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT896180.17253850055768222No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAA889880.17132558289213132No Hit
AGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT873590.16818932435692116No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA868530.16721513969220886No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAATAGAT868160.16714390484518443No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATTGG849930.16363414467963003No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAATAGATCGG848370.163333803162446No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG842820.16226528045707975No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA839340.16159528784182306No Hit
TAGGAATAATGGAATCTGTAGGCATCATCAATAGATCGGAAGAGCACACG824720.15878054875129066No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG810580.15605822243527645No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG806300.15523420852915615No Hit
TTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACA804850.15495504493946588No Hit
TCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC797760.1535900312491872No Hit
TATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCTGTAGGCACCA783290.1508041711506917No Hit
AATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG770480.14833790523073823Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATTGGAAGAGCACACGT732110.14095065906769255No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGCTGTAGGCACCATCAATA717550.13814747157397494No Hit
TTCTGATCGAGGCCCTGTAGGCATCATCAATAGATCGGAAGAGCACACGT709220.13654372488285765No Hit
ATTGATCATCGACACTTCGAACGCACTCTGTAGGCACCATCAATAGATCG707030.13612209159911853No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT703140.13537316307229424No Hit
ACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCAC701980.13514983220054205No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA697040.13419875072945928No Hit
ACCTCGGGCCGATCGCACTGTAGGCACCATCAATAGATCGGAAGAGCACA665260.12808025494990255No Hit
CTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC662310.12751230143984302No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAA660380.12714072507563456No Hit
CTGTAGGCACTATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA655830.1262647289838478Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGA629470.12118972744989201No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA624510.12023479544653766No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC624250.12018473852700698No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG620650.11949164271812075No Hit
TTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGGA620120.11938960361292361No Hit
TAGGAATAATGGAATCTGTAGGTACCATCAATAGATCGGAAGAGCACACG612130.11785131597042338No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGG609640.11737192470261039No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAAT598820.11528878674368015No Hit
CACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGCAC595920.11473045956429957No Hit
ACGTAGCAGAGCAGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACA594450.11444744544233769No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAG591740.11392569831953722No Hit
TCAGCCCTCGACACACTGTAGGCACCATCAATAGATCGGAAGAGCACACG591170.1138159581497969No Hit
CCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAG582460.11213905134551941No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCTGTAGGCACCATCAATAGATC581560.11196577739329786No Hit
TATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCAT580530.11176747498131095No Hit
TAGGAATAATGGAATCTGTAGGCACTATCAATAGATCGGAAGAGCACACG571860.11009826924157663No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA567860.10932816278725861No Hit
TCGGGCCGATCGCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT558420.10751071155506806No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA556350.10711218146495849No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGCTGTAGGCACCATCAATAGA555510.10695045910955171No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA553970.10665396812463926No Hit
GCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA547560.10541987253159463No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCATC540600.10407988730108127No Hit
AAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAA537670.1035157843232933No Hit
GCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGGA533770.10276493053033324No Hit
CTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC529130.10187160704332432No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)