FastQCFastQC Report
Thu 29 Jun 2023
SRR1610248.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1610248.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50730087
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG17714823.491975087683173No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT10849752.1387209527158904No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA9408631.8546449565521146Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA7661571.5102615534643178No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA6334141.2485963211535591No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC5417101.0678278552922649No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG5359091.056392826608005No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4890510.9640255495717955No Hit
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG3756050.740398887941982No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA3241780.6390251213249447No Hit
GTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2782310.5484536227978477No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA2664070.5251459552986771No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA2663210.52497643065347No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT2537920.5002790553069622No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA2499810.49276674806412213No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC2493930.4916076725829388No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG2450720.48309004476968476No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG2255830.4446730004622306No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG2236590.44088037932992313No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG2214330.43649245072258597No Hit
CCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA2036420.4014225325495696Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA1938100.3820415289254284No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA1804950.3557947771703999No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA1762610.3474486452191576No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG1752980.3455503634361991No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAATA1671410.3294711479599867No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA1552310.30599395581560895No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG1531750.3019411340650766No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA1454060.2866267507091009No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA1442630.2843736499012903No Hit
AGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC1267650.24988129825206096No Hit
CTGTAGGCACCATTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1248340.24607487860212027Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA1210680.23865127611549336No Hit
TGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1199340.23641591625892538No Hit
GCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAA1190880.23474826684212075No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACCA1143230.2253554187675649No Hit
TCCCACGGCGCGACCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCA1099300.21669586334437No Hit
GTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1076130.21212855400780212No Hit
TCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACA1057690.20849363021987324No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG1041600.20532194238105683No Hit
AAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCA1039310.20487053373277284No Hit
TTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGAA1035640.20414709716543555No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAAT1029300.20289734571123444No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCACC1009180.19893125750011031No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA1002520.19761842710815772No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC962150.189660624867448No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC960530.18934128774508113Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAG960180.18927229515691546No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA944630.18620705302555465No Hit
CCGCCACGCAGTTTTATCCGGTAACTGTAGGCACCATCAATAGATCGGAA901230.1776519720930106No Hit
CACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCA863400.1701948589207032No Hit
AGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGA856250.16878543890531866No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCAC845480.1666624384066205No Hit
TAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATCG818090.16126327557845505No Hit
CCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAATAGATCGGA787510.15523529458957958No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA782830.15431276512496422No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG771740.1521266856885146No Hit
CTGTAGGCACTATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA768190.15142690372283416Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCTAGAGCTAATACACTGTAGGCACCATCAATAGATCGGAAGAGCACAC707700.1395030132710003No Hit
CCGTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC702060.13839124699313052Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC697700.13753179646626668No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCATC689120.1358404924478052No Hit
TTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT681500.13433842524259815Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATTGG677460.13354205365348576No Hit
TCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT663290.13074883944117818No Hit
TTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGGA662410.1305753723623616No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA651530.1284306884788114No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAAT648930.12791817210958065No Hit
TCCCACGGCGCGACCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC647480.12763234567289428No Hit
ACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATA644140.12697395926011323No Hit
ACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAG643620.1268714559862671No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAATAGAT630500.12428521953845653No Hit
TATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCAT627510.12369582571384118No Hit
GCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGGA614080.1210484815450839No Hit
TAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGA612740.12078433849324957No Hit
ACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCAC609820.12020874318626736No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATCTGTAGGCACCATCAATA608220.11989334849750997No Hit
TCCCACGGCGCGACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA603800.11902207066981771No Hit
CTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGA601620.11859234540638576No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA600670.11840507980993606No Hit
CGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGG596070.11749832007975859No Hit
AAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAA593250.1169424369408237No Hit
TTCTAGAGCTAATACATGCTGTAGGCACCATCAATAGATCGGAAGAGCAC581060.11453952365585339No Hit
ACCTCGGGCCGATCGCACTGTAGGCACCATCAATAGATCGGAAGAGCACA570340.1124263792411789No Hit
CTGTAGGTACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA565490.11147033909088308Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGATC555720.10954446027265832No Hit
ATCGGGGATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA552880.10898463470011396Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGGAA545710.10757127225111993No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATCTGTAGGCACCATCAAT545190.10746876897727378No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT537230.1058996804007058No Hit
TGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG535980.10565327830011409No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGA528610.1042004915150254No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA526970.10387721195904909No Hit
TCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGA520090.10252101479739231No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)