FastQCFastQC Report
Thu 29 Jun 2023
SRR1610250.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1610250.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44713983
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA19020554.253825922866232No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG13372712.9907221640264074No Hit
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG7988031.78647247774818No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT7740721.731163157618949No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA5896361.3186836878298227Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT5893251.3179881559645448No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG5742621.2843007074543102No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC4075850.9115381199657386No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA3964380.8866085582221562No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC3635530.8130633318888189No Hit
CCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA3229360.7222259757087621Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG2666120.5962609056768663No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA2639020.5902001617704242No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG2463740.55099989638588No Hit
GTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2242420.501503075670982No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG2061480.461036987020369No Hit
TTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGGA1911340.42745912391656093No Hit
CCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAATAGATCGGA1786330.39950142665662325No Hit
TTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGAA1689250.37779009756299275No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA1684780.37679041028395976No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA1539300.3442547267596358No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1373460.30716565777644994No Hit
TCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACA1358780.30388256845738837No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1347800.30142696077868975No Hit
CCGCCACGCAGTTTTATCCGGTAACTGTAGGCACCATCAATAGATCGGAA1298430.29038567197200926No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG1294380.2894799150413418No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1266110.28315750802159584No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA1259740.2817328977380521No Hit
CCGTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1185460.2651206446985499Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC1177220.263277820721093No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA1111220.24851733740651108No Hit
TGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1107310.24764289059196537No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT1071700.23967893891268868No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA1042040.2330456671685902No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA1032450.23090092421424413No Hit
GTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1009540.2257772473545915No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA980770.2193430184915533No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG962020.21514969936809256No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAATAGATCGG959320.21454586141431414No Hit
TTCTAGAGCTAATACACTGTAGGCACCATCAATAGATCGGAAGAGCACAC948700.212170765462786No Hit
ATTGATCATCGACACTTCGAACGCACTCTGTAGGCACCATCAATAGATCG948550.2121372189097983No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA918900.20550618360256567No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAATAGAT906720.20278220349996554No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT904250.20222980359410164No Hit
AGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGA897410.20070008077786314No Hit
TCCCACGGCGCGACCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCA885010.19792689906421446No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC882210.19730069674177764No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAATA881000.19703008788101029No Hit
ATCGGGGATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA852160.19058020395991115Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG850100.19011949796554692No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA832760.18624151644017042No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA829470.18550572871130716No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA818770.1831127412648522No Hit
TTCTAGAGCTAATACATGCTGTAGGCACCATCAATAGATCGGAAGAGCAC806140.18028812150328902No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCAC767510.1716487658905269No Hit
TAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGA766510.17142512220394232No Hit
GCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGGA762050.17042767136177514No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA750400.16782222241306483No Hit
AAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCA749600.1676433074637972No Hit
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA747890.16726087675973755No Hit
TTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC744990.16661231006864227No Hit
TTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCGGAAGAGCACA729010.16303848395702078No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGCTGTAGGCACCATCAATA727730.1627522200381925No Hit
TCCGGGACGGCTGGGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC721130.16127617170673433No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAG695850.15562245930987628No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA694420.15530264883806033No Hit
CGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGG689220.15413970166782057No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG682030.15253170356127746No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA676730.1513463920223792No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT668090.14941411057028847No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG652760.14598565285494697No Hit
CCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAG649330.1452185550099619No Hit
CTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGA645420.14434410819541618No Hit
TCCCACGGCGCGACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA636690.14239169881153285No Hit
CGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGGAA631560.14124440669935398No Hit
CTGTAGGCACCATTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA618290.13827665497837668Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAA617070.1380038096807435No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCATCATCAATAGATCGGA607690.13590603190058018No Hit
ACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCAC588810.1316836390978634No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACCA588790.13167916622413173No Hit
AAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAA571560.12782578550427948No Hit
CACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCA549510.12289444221508963No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCACC544760.1218321347038129No Hit
TATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCAT535710.11980815934022251No Hit
ATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACGATCT526490.11774616454991271TruSeq Adapter, Index 6 (97% over 37bp)
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGG515750.11534423135599438No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATTAATAGATCGGA512500.11461738937459452No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAATAGA508180.11365124864854916No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC505110.11296466253073452Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACAC501780.11221992905440788No Hit
ATGTTGGAACAATGTAGGTAAGGGACTGTAGGCACCATCAATAGATCGGA499170.11163621903242213No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAAT498900.1115758352370443No Hit
TCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGAAGAGCACA498900.1115758352370443No Hit
ACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATA496900.11112854786387515No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC493260.1103144848447073No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCATC493030.11026304679679286No Hit
ATCGGGGATTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG484690.1083978584506775Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATCG475450.10633139078663602No Hit
TCCCACGGCGCGACCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC471040.10534512212879805No Hit
ACCTCGGGCCGATCGCACTGTAGGCACCATCAATAGATCGGAAGAGCACA468710.104824032339056No Hit
CTAAACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATAGATCG468560.1047904857860683No Hit
CCCGTGCCTTGGAAAGCGTCGCCTGTAGGCACCATCAATAGATCGGAAGA466950.10443041945066714No Hit
TATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCTGTAGGCACCA464500.10388249241853494No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATCTGTAGGCACCATCAATAG459630.10279334766486806No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACTATCAATAGATCGGA456840.10216938177929709No Hit
CTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC452210.10113391151041051No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)