FastQCFastQC Report
Fri 30 Jun 2023
SRR1610251.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1610251.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58074106
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG28362064.883770401906832No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA18204293.1346655598968667No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT7527931.296262744018823No Hit
TTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGAA6722211.157522769270008No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA5277740.9087940157012491No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG4775920.8223837315722089No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG4407230.7588976057590968No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC4182700.7202349356871718No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG3540500.6096520883162626No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC2929410.5044261895310106No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2472830.42580595213984007No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG2388230.41123835810748427No Hit
CCGCCACGCAGTTTTATCCGGTAACTGTAGGCACCATCAATAGATCGGAA2282260.39299098293480406No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA2218500.3820119073378418No Hit
TTCTAGAGCTAATACATGCTGTAGGCACCATCAATAGATCGGAAGAGCAC2099050.36144335997182636No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA2081750.3584644075278576No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT2061940.3550532486888391No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1979730.3408971977975864Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGA1931520.33259573552453825No Hit
GCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGAA1914760.3297097677233292No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG1844950.31768891973989233No Hit
TTCTAGAGCTAATACACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1828390.31483739069526095No Hit
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1803990.31063586239278485No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAATA1802580.31039306915891224No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1799090.30979211285663183No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA1730670.2980106142314098No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA1663400.28642713845650936No Hit
TAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGA1656710.2852751620489862No Hit
ACCTCTAGCATCACCAGTATTAGAGGCCTGTAGGCACCATCAATAGATCG1571660.2706300808143306No Hit
TTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGGA1557010.268107441895016No Hit
GTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1534880.2642967934796964No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCAA1532270.2638473677063578No Hit
CCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAG1429210.24610107644188273No Hit
CCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAATAGATCGGA1378960.23744833885174227No Hit
AAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAA1360440.2342593099926497No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA1351370.23269751238185224No Hit
TCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACA1326260.22837372649352536No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA1297830.2234782572460091No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA1280530.22049930480204033No Hit
CGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGGAA1274070.21938693296458148No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG1237260.21304847981646072No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG1229630.2117346412530225No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATCG1150950.19818643441536576No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA1104360.19016392607059676No Hit
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG1057320.18206393052352798No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCTGTAGGCACCATCAATAGATCGGA1034350.1781086393305822No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA1033060.17788650935065622No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA1032220.17774186657302998No Hit
AGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC1013960.17459760809748842No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA953230.16414027966267788No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAA937580.16144544696047497No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATTAATAGATCGG929000.15996802430329277No Hit
TTCTAGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGCA925040.15928613692305485No Hit
TTGGAACAATGTAGGTAAGGGACTGTAGGCACCATCAATAGATCGGAAGA923660.15904850950266888No Hit
ATTGATCATCGACACTTCGAACGCACTCTGTAGGCACCATCAATAGATCG911630.15697701829452182No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG890380.15331790040814405No Hit
TAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATCG870530.14989985381780996No Hit
CGGGGCGCGGGACATGTGGCGTACCTGTAGGCACCATCAATAGATCGGAA858880.14789379624716048No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGACTGTAGGCACCATCAATAGAT856550.1474925847330306No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATC851020.14654035311365793No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG842890.1451404176587755No Hit
AAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCA827970.14257128641808106No Hit
TATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCTGTAGGCACCA827890.14255751091545No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATTAATAGATCGGA824410.1419582765509985No Hit
GTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT822840.14168793231186375No Hit
CGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGATCGGAAG815530.14042919575894977No Hit
CGGGGCGCGGGACATGTGGCGTACTGTAGGCACCATCAATAGATCGGAAG780350.13437141847693704No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC775820.13359138064045273No Hit
CCGTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA768850.13239118997372082Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CCGCCACGCAGTTTTATCCGGTACTGTAGGCACCATCAATAGATCGGAAG764280.131604264385921No Hit
CGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCGG758080.1305366629320131No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCA754140.12985821942743295No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCATCATCAATAGATCGG742280.12781600116237693No Hit
CGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGG733560.12631447137559035No Hit
GTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATAGATC724360.12473028857301738No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCTGTAGGCACCATCAATAGATCGG718590.12373673044575151No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT715720.12324253428886188No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATC712850.12274833813197226No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC704010.12122614509123911No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAAT698070.12020331402088222No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA677370.11663890271509303No Hit
TGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG672350.11577448992499341No Hit
TGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC669800.11533539577862809No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC661750.11394923582637674No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACTATCAATAGATCGG658870.11345331773165823No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA658030.11330867495403202No Hit
AACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAAG654590.11271632834089602No Hit
TCCGGGACGGCTGGGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC652840.11241498922084138No Hit
AACGATGCCGACCGGCGATGCGGCGGCGCTGTAGGCACCATCAATAGATC652150.11229617551064841No Hit
GCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCGGA648040.11158845906297722No Hit
ACCCGTCGCCGCGCTCTCCCCCCTCCCGGCGCCTGTAGGCACCATCAATA647220.11144726016100877No Hit
CGCCACGCAGTTTTATCCGGTAACTGTAGGCACCATCAATAGATCGGAAG644620.110999556325499No Hit
ATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCAGCATCT637230.10972704426995399TruSeq Adapter, Index 11 (97% over 36bp)
ATTGATCATCGACACTTCGAACGCACTGTAGGCATCATCAATAGATCGGA632490.10891084573906311No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA623560.10737315525787001No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATCTGTAGGCACCATCAAT621800.10707009419998648No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGG619340.1066464974940811No Hit
ACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAG618990.10658622967007017No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAG618130.10643814301678617No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCAA616870.10622117885034682No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCA616380.10613680389673152No Hit
ATAACTGTGGTAATTCTAGAGCTCTGTAGGCACCATCAATAGATCGGAAG611350.10527066916880305No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACTATCAATAGATCGGA603130.10385523627346067No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA602770.10379324651162086No Hit
TTCTAGAGCTAATACATCTGTAGGCACCATCAATAGATCGGAAGAGCACA595880.10260683134752002No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCA594280.10233132129489862No Hit
TAAGGCCTACTTCACAAAGCGCCTTCCTGTAGGCACCATCAATAGATCGG589480.10150479113703446No Hit
CTAAACCATTCGTAGACGACCTGCTTCCTGTAGGCACCATCAATAGATCG586940.101067418928498No Hit
CTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC582470.10029771271898702No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)