FastQCFastQC Report
Fri 30 Jun 2023
SRR1610252.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1610252.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36688083
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA29126477.938945733414308No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA21486525.856539301876307Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA7400432.01712092725041No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG5939811.619002551864048No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG4606711.2556420568499043No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT4396911.1984572756227136No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC3366650.9176412951311738No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA2555190.6964632085028809No Hit
TTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGAA2508280.6836770403076116No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG2261590.6164372229532952No Hit
ACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCAC2203650.600644628938503No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA2155630.5875559101847867No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATC1947600.5308535744426875No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCG1906420.5196292212923744No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA1587800.4327835826145509No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC1546630.42156195514494443No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA1503810.4098905903587277No Hit
TAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATCG1455700.3967773404786508No Hit
TTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGGA1454370.3964148249446557No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG1446020.39413888155453636No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA1359430.37053721231496345No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCG1339970.3652330376596673No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACTATCAATAGATCGGA1264510.3446650510466846No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCATCATCAATAGATCGGA1260300.3435175394691513No Hit
GCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAA1248470.34029305919309005No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA1243070.33882119161145596No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1194420.3255607549732157No Hit
TAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1145200.3121449545346918No Hit
TTCTAGAGCTAATACATGCTGTAGGCACCATCAATAGATCGGAAGAGCAC1142120.31130544487701906No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATTAATAGATCGGA1136840.30986628546386574No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG1051780.2866816453724224No Hit
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1021060.2783083542413486No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT984840.2684359387215734No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA973240.26527414910176694No Hit
CTGTAGGCACCATTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA965470.2631562951926379Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA961240.2620033322536912No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT888230.24210313741385722No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA857380.23369441243359595No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT852240.23229341255033686No Hit
TCCCACGGCGCGACCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCA839770.22889448870904486No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG834910.22756980788557418No Hit
TCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG821810.2239991661597582No Hit
CCGCCACGCAGTTTTATCCGGTAACTGTAGGCACCATCAATAGATCGGAA812100.2213525301935236No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA809870.22074470339592286No Hit
CCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAATAGATCGGA809530.22065203025189406No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGTACCATCAATAGATCGGA799970.21804627949626043No Hit
ACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAG799260.2178527561660826No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG783700.2136115969864111No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG756950.206320401095909No Hit
ATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATCGATCT756880.2063013213309619TruSeq Adapter, Index 3 (97% over 36bp)
TCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGAAGAGC739030.20143598126944928No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC715120.1949188786996584No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA714690.19480167442926904No Hit
AGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGA712060.1940848204033991No Hit
CTGTAGGCACTATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA696280.18978369624817956Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGTAGGCACCATCA685310.186793624512897No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG661590.18032830987653403No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA650990.17743908832740046No Hit
ACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAG638620.17406742129317576No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA636990.17362313533797882Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACCA611180.16658815343390931No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGCTGTAGGCACCATC603660.16453844154244854No Hit
CTGTAGGCATCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA593040.16164376863190152Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCAC588850.16050170841578176No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC586800.15994294387090216Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCCCCTTGTAAATTTAACTGTTAATCCAAAGACTGTAGGCACCATCAATA569570.15524659601320678No Hit
TTCTAGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGCA568500.1549549481775867No Hit
ATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAG564200.15378290547369292No Hit
ATGTTGGAACAATGTAGGTAAGGGACTGTAGGCACCATCAATAGATCGGA552360.15055569951692488No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCACC537350.14646445277612352No Hit
ATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCG536180.14614554813343614No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA527920.14389413586967734No Hit
CTGTAGGTACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA508960.13872624524971774Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGG488750.13321764454141688No Hit
ATTGATCATCGACACTTCGAACGCACTCTGTAGGCACCATCAATAGATCG482140.13141596959426852No Hit
AGCTAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCA465730.126943127554525No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA463740.12640071709388576No Hit
TATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCTGTAGGCACCA459900.12535405570250155No Hit
TTATACCCACACCCACCCAAGAACAGGGTTTGTTCTGTAGGCACCATCAA454450.12386855971733383No Hit
AGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC441030.12021069620890248No Hit
ATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCAC440680.12011529738416694No Hit
ATTATACCCACACCCACCCAAGAACAGGGTTTGTTCTGTAGGCACCATCA439050.11967101142897No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC428350.11675453307276916No Hit
TCCCACGGCGCGACCGCTCTCTGTAGGCACCATCAATAGATCGGAAGAGC418320.11402067532391921No Hit
TCCCCTTGTAAATTTAACTGTTAATCCAAAGAGCTGTAGGCACCATCAAT417110.1136908679584049No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAAT416970.11365270842851069No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCTGTAGGCACCATC406500.11079892072856463No Hit
GGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAA405760.11059722035626662No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACACTGTAGGCACCATCAATA396470.1080650629797147No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT393600.10728279261688325No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA390150.10634243277306149No Hit
AAAAAGCTCGTAGTTGGATCTTGGCTGTAGGCACCATCAATAGATCGGAA389070.10604805925673466No Hit
ACGATCAAAAGGGACAAGCATCAAGCACGCAGCCTGTAGGCACCATCAAT387720.10568009236132615No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAAT377070.10277724240865896No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAG370190.1009019740824289No Hit
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC368380.1004086258745108No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)