FastQCFastQC Report
Thu 29 Jun 2023
SRR1610253.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1610253.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33936769
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA455531913.42296021168073Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA18427095.429830400177459No Hit
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG6441851.8981918991757878No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG5665871.6695372502903858No Hit
TAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACAC5257791.5492900929961835No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA4144781.2213242810474974No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT4113871.2122161658936947No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT2668140.7862091998209966No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG2615340.7706508536507999No Hit
TTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGAA2590140.7632252793422969No Hit
CTGTAGGCACCATTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2231250.6574727252320337Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCAC2031060.5984836093265096No Hit
CTGTAGGCACCATCAATAGATTGGAAGAGCACACGTCTGAACTCCAGTCA1948020.5740145739861093Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1779000.52421018630265No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC1757740.5179455946439686No Hit
TTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGGA1653660.4872767940872627No Hit
TCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1616360.47628576544808965No Hit
CTGTAGGCACTATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1610760.4746356378239779Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTAGGCATCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1400040.4125436926538293Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1371180.40403964207671034Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1318400.3884871892194569No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1226750.3614810826569848No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG1202370.35429713417915537No Hit
CTGTAGGTACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1195710.352334660969051Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGAAGAGCACA1164370.3430998395869684No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA1162810.3426401611773944No Hit
TAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATCG1157530.3410843265603747No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1087100.32033102503069755Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCAC1028930.3031903243352365No Hit
TCCCACGGCGCGACCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCA895900.2639909532931671No Hit
TTCTAGAGCTAATACATGCTGTAGGCACCATCAATAGATCGGAAGAGCAC888780.26189293388536783No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC887240.2614391487887371No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATTGGA878510.2588667176890057No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATTAATAGATCGGA878140.25875769139955546No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG878070.25873706480425407No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCG875480.25797388077810235No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCATCATCAATAGATCGGA842580.24827938098644572No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACTATCAATAGATCGGA819120.24136652490400604No Hit
CCGCCACGCAGTTTTATCCGGTAACTGTAGGCACCATCAATAGATCGGAA806650.23769204428388568No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG750890.22126148779808708No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCG746060.21983825272229068No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA710780.20944244869038653No Hit
TCCCACGGCGCGACCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC705390.20785420085217893No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG679020.2000838677364955No Hit
TATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGTCTGTAGGCACCA663960.19564620309022346No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG649520.1913912311451924No Hit
CCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAATAGATCGGA634210.18687990008712968No Hit
TCCCACGGCGCGACCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA611590.18021456314830678No Hit
AGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGA593700.174942994720564No Hit
GCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAA572370.16865777646658112No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC571820.16849571036064157No Hit
TCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACA569720.16787691250159967No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA566190.1668367427671149No Hit
TCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGGAAGAGCAC562250.16567576011729343No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA557560.1642937782320998No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA535560.15781113399451788No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA534990.15764317457563506No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGTACCATCAATAGATCGGA528850.15583392750205535No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA528140.15562471489256977No Hit
AGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC528090.1556099816102116No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG527480.1554302355654423No Hit
ATGTTGGAACAATGTAGGTAAGGGACTGTAGGCACCATCAATAGATCGGA523300.15419853316030174No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA511170.15062423886021678No Hit
ATTGATCATCGACACTTCGAACGCACTCTGTAGGCACCATCAATAGATCG500580.14750372965676256No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA487240.14357288992361059No Hit
TTCTAGAGCTAATACACTGTAGGCACCATCAATAGATCGGAAGAGCACAC473080.1394004243597851No Hit
TCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGAAGAGC470640.13868144018070783No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA466540.13747331102734028Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAAT459970.1355373577254806No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCAC456920.134638627501634No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC436730.12868932808541672Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC436690.12867754145953023No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT435160.1282267030193711No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT427080.12584580459029554No Hit
ATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCAGTATCT409180.12057128950608116TruSeq Adapter, Index 1 (97% over 37bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACCA385760.113670220049528No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA380770.11219983847018554No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA378900.11164881370999108No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCACC373540.11006940584119837No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA361120.1064096585034362No Hit
GTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG360270.10615919270334781No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)