FastQCFastQC Report
Fri 30 Jun 2023
SRR1610254.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1610254.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42154513
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA467742611.09590804666632Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA36298458.61080995052653No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG11281292.6761760953091787No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA5135551.2182681365575259No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG5040171.1956418521547147No Hit
TTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGGA2808540.6662489494303967No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCG2803810.6651268868887182No Hit
TTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGAA2780890.659689746623333No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT2753030.6530807270860892No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA2523500.5986310409990978No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA2488520.5903329970862194No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCG2323650.5512221194442456No Hit
CTGTAGGCACCATTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2128500.5049281437553318Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATTAATAGATCGGA2112250.5010732777294806No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG2102170.49868207468082953No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA1858000.4407594508327021No Hit
TAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATCG1723160.40877236560650104No Hit
CTGTAGGCACCATCAATAGATTGGAAGAGCACACGTCTGAACTCCAGTCA1699230.4030956305912015Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA1682610.39915299223122325No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATTGGA1659570.39368738526287805No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC1653310.39220237225845783No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCATCATCAATAGATCGGA1622740.38495047967936435No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACTATCAATAGATCGGA1609410.3817883034255431No Hit
CTGTAGGCACTATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1547390.36707576244564843Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1448230.3435527768995932No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1410610.3346284655215919Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1390950.3299646706866238No Hit
CTGTAGGCATCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1350240.32030734170739916Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1248480.2961675775972077Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGTAGGTACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1177770.2793935728779502Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGTTGGAACAATGTAGGTAAGGGACTGTAGGCACCATCAATAGATCGGA1172570.27816001574967786No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG1148700.27249751408585837No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCAC1066710.25304763928834856No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGTACCATCAATAGATCGGA1031330.24465470636560313No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA1018350.24157555799541558No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA1002380.23778711427647142No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT992370.2354125168045471No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG979280.23230727395664613No Hit
AGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGA955220.22659970001313975No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC932840.22129065991107524No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAAT917170.2175733829495314No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA899630.21341249986685887No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG880220.2088080106630576No Hit
ATTGATCATCGACACTTCGAACGCACTCTGTAGGCACCATCAATAGATCG878920.2084996213809895No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCACC842400.19983625478012285No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG840630.1994163709114609No Hit
GGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAA814220.1931513240349853No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACCA812510.19274567351780342No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA803250.19054899293937994No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA780950.18525893063928883No Hit
GCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAA750880.17812564932252922No Hit
CCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAATAGATCGGA740840.17574393517486492No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGG688000.16320909697142036No Hit
TCCTAAGGCGAGCTCAGGGAGGACACTGTAGGCACCATCAATAGATCGGA682340.16186641748180083No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATTAATAGATCGG659440.15643402166690906No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA654540.15527163129603702No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGA642710.1524652888292174No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT587020.13925436643046973No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAG586670.139171338546836No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC558240.13242710217053155No Hit
ATGCTAAGACTTCACCAGTCAAAGCGACTGTAGGCACCATCAATAGATCG549350.13031819392623514No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGA536340.12723192888030754No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT531660.12612172746486242No Hit
ATGTTGGAACAATGTAGGTAAGGGCTGTAGGCACCATCAATAGATCGGAA530350.12581096595754765No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGATCGG521980.12382541342607849No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAAT513160.12173311075850883No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATTGG512670.1216168717214216No Hit
ATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCATATCT505880.12000613077892751TruSeq Adapter, Index 10 (97% over 37bp)
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG505480.11991124176906041No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA502420.11918534084357706No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGAT488760.11594488115661543No Hit
CCGCCACGCAGTTTTATCCGGTAACTGTAGGCACCATCAATAGATCGGAA485890.11526405251081895No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACTATCAATAGATCGG475620.11282777718248102No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCATCATCAATAGATCGG463870.11004041251763481No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATAGA461670.10951852296336574No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA458300.1087190830552354No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT454910.10791489869661167No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTCCTGTAGGCACCATCAATAGATCG446200.10584869050675547No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA443880.10529833424952625No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCACC441980.1048476114526575No Hit
TAAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATA441730.10478830582149057No Hit
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC439180.10418338838358777Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCTGTAGGCACCATCAA434270.10301862578746905No Hit
ACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAG427680.10145532934990852No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)