FastQCFastQC Report
Thu 29 Jun 2023
SRR1610255.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1610255.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41611510
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA33952778.15946597467864Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA31340697.5317358105966346No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG16544373.9759119532071776No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA13517753.248560314201528No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG9525732.2892055587504516No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA5554591.3348686457184562No Hit
TTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGAA3694310.8878096468981779No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG3618840.8696728381161848No Hit
TTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGGA2567410.6169951535044029No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC2374230.5705704984029659No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT2083120.5006114894652945No Hit
ACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCAC1968500.473066226147525No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCG1883560.45265360473580507No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCG1873030.450123054895148No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA1819280.4372059557559915No Hit
TTCTAGAGCTAATACATGCTGTAGGCACCATCAATAGATCGGAAGAGCAC1763410.42377938219497446No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1729990.41574795050696306No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATTGGA1726670.4149500943368794No Hit
CTGTAGGCACCATTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1692040.40662787771940984Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATTAATAGATCGGA1682070.4042319060279236No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG1672710.40198252839178394No Hit
CTGTAGGCACCATCAATAGATTGGAAGAGCACACGTCTGAACTCCAGTCA1495550.35940776962912424Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1478170.3552310406423607No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACTATCAATAGATCGGA1477110.3549763034314304No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCATCATCAATAGATCGGA1425660.3426119359763681No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC1341910.32248529313163593No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA1310580.3149561263217797No Hit
TCCCACGGCGCGACCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCA1261750.3032213923503377No Hit
TAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATCG1259730.30273594974082896No Hit
ACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAGATC1249850.3003616066804593No Hit
CTGTAGGCACTATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1203040.28911231531852605Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA1148780.2760726539363748No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1124890.2703314539655014No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG1124770.2703026157906791No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1090070.2619635769045632No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1058850.2544608450882941Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1038180.24949346947515244No Hit
CTGTAGGCATCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1028180.24709028823996054Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1022040.24561473496155273No Hit
ATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCAC1014560.2438171553976292No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG1007080.24201957583370565No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG980720.23568479009773977No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGTACCATCAATAGATCGGA945310.22717512534392528No Hit
ACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGAAGAGC900700.21645453385373423No Hit
CTGTAGGTACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA893440.2147098242769849Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC879290.21130932282918835Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAAT869210.20888691614411492No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC847620.20369844785733562No Hit
ATGTTGGAACAATGTAGGTAAGGGACTGTAGGCACCATCAATAGATCGGA841070.20212436414828494No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA823270.19784670154964334No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATTGG767850.18452827114420986No Hit
TCCCACGGCGCGACCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC744090.17881831252939392No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCAC729570.17532889337589527No Hit
CCGCCACGCAGTTTTATCCGGTAACTGTAGGCACCATCAATAGATCGGAA706030.16967180474825355No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT704100.1692079907698615No Hit
TCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGAAGAGC694180.16682403498455117No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA693190.16658612004226717No Hit
CCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAATAGATCGGA685950.16484621682798822No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA676690.16262087100420053No Hit
AGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGA673570.16187107845882065No Hit
TCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGAAGAGCACA673210.16178456393435375No Hit
ATTGATCATCGACACTTCGAACGCACTCTGTAGGCACCATCAATAGATCG662910.15930928726210608No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG662630.15924199818752072No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA643930.15474804927771185No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGG640170.1538444531332797No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG636930.15306582241307753No Hit
TCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAAGA628950.1511480837873944No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTGTAGGCACC617810.14847093989139062No Hit
TCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGGAAGAGCAC617340.1483579903733366No Hit
TCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG608700.14628164178613082No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGTACCATCAATAGATCGG604950.14538044882293383No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA596710.1434002274851357No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCCTGTAGGCACCA579230.1391994666860203No Hit
TCCTAAGGCGAGCTCAGGGAGGACACTGTAGGCACCATCAATAGATCGGA564420.1356403552767011No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACTATCAATAGATCGG558850.13430178332869921No Hit
ACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAGA555740.1335543939645545No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATTAA527780.12683509923095798No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATTAATAGATCGG515750.12394407220502211No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATTGG508120.1221104449225707No Hit
GGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAA506120.12162980867553232No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGTACCATCAA497300.11951020282609306No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA495920.1191785638156366No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCATCATCAATAGATCGG492850.11844078717643267No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACTATCAA483350.11615776500300037No Hit
AAGTCAGAATCCCGCCCAGGCGGAACGATACCTGTAGGCACCATCAATAG481400.11568914466213796No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATTAATAGATCGG464820.1117046701741898No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCATCATCAA457560.10995996059744047No Hit
ACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGAAGA456210.10963553113068956No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAAT450630.10829455600145249No Hit
AGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC447440.10752794118742627No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAAT439750.10567989481756371No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA438520.10538430352563509No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT434910.10451675509973082No Hit
GCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAA432000.10381742936028998No Hit
TCCCACGGCGCGACCGCTCTCTGTAGGCACCATCAATAGATCGGAAGAGC430660.10349540307477427No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACTATCAATAGATCGG429680.10325989131372547No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGAT429230.10315174815814182No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGATCGG424900.10211117068330373No Hit
ATGTTGGAACAATGTAGGTAAGGGCTGTAGGCACCATCAATAGATCGGAA422920.10163534079873575No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)