FastQCFastQC Report
Fri 30 Jun 2023
SRR1610256.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1610256.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42335858
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA32769867.740450187639992Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA22241095.2534874809907No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG6258571.478314198805183No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA5572291.316210480486778No Hit
TTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGAA3986790.9417052560975615No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG3630400.8575236623289884No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCG2994780.7073861595057315No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCG2756230.6510391262177797No Hit
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC2586970.6110588333889442No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG2049590.48412624588829634No Hit
GCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAA2024930.47830139641908287No Hit
ACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCAC1873760.44259407710598425No Hit
TAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATCG1795470.42410147917635216No Hit
CTGTAGGCACCATCAATAGAGCGGAAGAGCACACGTCTGAACTCCAGTCA1773600.4189356455229985Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT1736870.4102597849794375No Hit
CTGTAGGCACCATTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1625920.3840526864956888Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGGA1621730.38306298173997083No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGG1588000.37509574035324855No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA1500990.3545434227410721No Hit
CTGTAGGCACCATCAATAGATTGGAAGAGCACACGTCTGAACTCCAGTCA1457670.3443109621163223Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA1385210.3271954474148132No Hit
ATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAG1247940.29477139686173365No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATTAATAGATCGGA1247210.29459896620023623No Hit
ATTGATCATCGACACTTCGAGCGCACTGTAGGCACCATCAATAGATCGGA1242460.293476985868575No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG1217420.28756237797282863No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATTGGA1188380.2807029445346307No Hit
ACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAG1183590.279571515947545No Hit
CTGTAGGCACTATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1181030.278966827600376Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC1148670.2713231889619433No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1118730.2642511697766938Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGA1115000.26337011995835774No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA1006830.23781967522661288No Hit
CTGTAGGCATCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1003730.23708743543121294Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATTGATCATCGACACTTCGAACGCACTGTAGGCACTATCAATAGATCGGA1001940.23666462600096588No Hit
TAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACAC998050.2357457831609318No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCATCATCAATAGATCGGA981420.23181767096818964No Hit
CCGCCACGCAGTTTTATCCGGTAACTGTAGGCACCATCAATAGATCGGAA978140.23104291402337943No Hit
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG955830.22577314956035616No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG904120.2135589173603143No Hit
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC895290.21147321497535257Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC885080.2090615477782451No Hit
GGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAA855330.202034407806262No Hit
CTGTAGGTACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA835530.19735752137112705Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG808300.1909256214908884No Hit
TCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG786520.18578104641223994No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCTGTAGGCACCATCAATAGATCGG773230.18264186354744483No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT735230.1736660208941555No Hit
TCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGAAGAGC727490.1718377834695118No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCA716960.16935053022900823No Hit
ATGTTGGAACAATGTAGGTAAGGGACTGTAGGCACCATCAATAGATCGGA713460.16852380787936316No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG687540.1624013383642774No Hit
GGGGGGGATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGA684630.16171397778214391No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGCTGTAGGCACCATCAATA667160.15758745222548698No Hit
CCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAATAGATCGGA661960.1563591790203No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGTACCATCAATAGATCGGA653480.1543561488703028No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG629550.14870373006258666No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATA613310.14486773836023353No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAAT600440.14182776217739582No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA596130.14080971265540432No Hit
TACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGA595280.14060893722763337No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTCCTGTAGGCACCATCAATAGATCG580790.1371863067001028No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG561850.1327125577565949No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA549090.12969856427617457No Hit
TCCTAAGGCGAGCTCAGGGAGGACACTGTAGGCACCATCAATAGATCGGA541950.12801205068289864No Hit
ACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAG528410.12481381622170028No Hit
TCTAAGTCAGAATCCCGCCCAGGCGGAACGATACTGTAGGCACCATCAAT503030.11881889815484546No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAATA502100.11859922621622551No Hit
ATTGATCATCGACACTTCGAACGCACTCTGTAGGCACCATCAATAGATCG468910.11075953627773412No Hit
TACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAG459360.10850376529513113No Hit
CTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAG451480.10664245897650167No Hit
ACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCTGTAGGCACCATC429910.1015474872388319No Hit
AACGATGCCGACCGGCGATGCGGCGGCGTTCTGTAGGCACCATCAATAGA429070.1013490738749171No Hit
CCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGAT426920.10084123014584942No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA426690.10078690267715845No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)