FastQCFastQC Report
Thu 29 Jun 2023
SRR1610258.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1610258.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32704287
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA749459422.916243365892676Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA22477416.872924641347479No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG6643922.031513483232336No Hit
CTGTAGGCACCATCAATAGAGCGGAAGAGCACACGTCTGAACTCCAGTCA4069701.2443934338027305Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CTGTAGGCACCATTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3660711.1193364343946712Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG3157660.9655186795541514No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA3062990.9365714042321118No Hit
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2648120.8097164753966354Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTAGGCACTATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2627850.8035185111970183Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2352570.7193460600440548Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTGTAGGCATCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2213070.6766911016895125Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTAGGTACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1868420.5713073640773761Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGGA1443860.4414895209303905No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCG1414130.4323989695907451No Hit
ATTGATCATCGACACTTCGAGCGCACTGTAGGCACCATCAATAGATCGGA1261600.385759824086671No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATTAATAGATCGGA1213030.3709085600918314No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCG1181880.36138381491087085No Hit
TTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGAA1158070.35410342381107407No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA993670.30383478471797903No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACTATCAATAGATCGGA973100.2975450894251264No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCATCATCAATAGATCGGA948970.29016685182587837No Hit
ATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGA913380.27928448646503135No Hit
CTGTAGGCACCATCAATAGACCGGAAGAGCACACGTCTGAACTCCAGTCA912120.2788992158734419Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA903330.2762114948416396No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA864420.2643139720489855No Hit
ATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACGATCT853520.26098107566142625TruSeq Adapter, Index 6 (97% over 37bp)
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG837980.2562294050318235Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCAC808820.24731314276932562No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC804020.24584544527755642No Hit
CTGTAGGCACCATCAATAGAACGGAAGAGCACACGTCTGAACTCCAGTCA714620.2185095794933551Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ATTGATCATCGACACTTCGAACGCACTGTAGGTACCATCAATAGATCGGA625500.19125932939617366No Hit
ATGTTGGAACAATGTAGGTAAGGGACTGTAGGCACCATCAATAGATCGGA623080.19051936524407334No Hit
ATTGATCATCGACACTTCGATCGCACTGTAGGCACCATCAATAGATCGGA595690.18214431643166537No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT593540.1814869102634771No Hit
ACGACGCTCTTCCGATCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC591590.18089065815744584No Hit
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG584660.17877166990370408No Hit
TCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG571020.17460096286459326No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC540490.1652657952763196Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG522870.15987812240028348No Hit
ATTGATCATCGACACTTCGAACGCACTCTGTAGGCACCATCAATAGATCG503080.1538269279498434No Hit
CTGTAGGCACCATCAATAGATTGGAAGAGCACACGTCTGAACTCCAGTCA486840.14886121810269096Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
AATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTACGATCTCGT471450.14415541301970597TruSeq Adapter, Index 6 (97% over 37bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGTAGGCACCATCAATAG462750.14149521131587428No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAG442130.13519022750748244No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC429740.1314017333568532No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCCTGTAGGCACCATCAATAGATCG417370.12761935461243964No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGTAGGCACCATCAATAGA411840.12592844479379722No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGG406640.12433843917771391No Hit
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT390570.11942471028339495No Hit
TAAGGCCTACTTCACAAAGCGCCTTCCCTGTAGGCACCATCAATAGATCG382210.11686847048523027No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG381720.11671864303294549No Hit
TAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACAC380580.11637006487865031No Hit
ATTGATCATCGACACTTCGAGCGCACCTGTAGGCACCATCAATAGATCGG373190.11411042228194732No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT365000.11160616343661611No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATTAATAGATCGG363120.11103131525233986No Hit
ACGCGCGCTACACTGACTGGCTCAGCGCTGTAGGCACCATCAATAGATCG358240.10953915613570783No Hit
ATACCGCAGCTAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGG347470.10624600988855068No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)