FastQCFastQC Report
Fri 30 Jun 2023
SRR1610259.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1610259.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33997375
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA674992219.85424462918093Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGA17075995.022737784902511No Hit
ACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCAC4747921.3965548810753772No Hit
ACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGC3872611.1390908856933808No Hit
CTGTAGGCACCATCAATAGAGCGGAAGAGCACACGTCTGAACTCCAGTCA3654061.0748065107967895Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGAA3534891.0397538045216725No Hit
CTGTAGGCACCATTAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3434041.010089749576254Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGTCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGC3369430.9910853411476621No Hit
GCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAG3182910.93622228186735No Hit
TCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGAAGAGC3106360.9137058375830487No Hit
ATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGG2794170.82187815971086No Hit
CTGTAGGCACTATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2494240.7336566426084367Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2439570.717575989322705Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTGTAGGCATCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2119720.6234951963203041Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC2055020.604464315259634Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTAAACCATTCGTAGACGACCTGCTTCTGTAGGCACCATCAATAGATCGG1936890.5697175149551987No Hit
ACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAG1749890.5147132683038028No Hit
CTGTAGGTACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1703430.501047507344317Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAG1645200.4839197143897139No Hit
TACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAG1379870.40587545361958094No Hit
TAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1296040.3812176675405087No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA1285150.378014479059045No Hit
GCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAA1029800.3029057390460293No Hit
TCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG970800.28555145801697923No Hit
ATTGATCATCGACACTTCGAGCGCACTGTAGGCACCATCAATAGATCGGA963490.2834012920115156No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACCATTAATAGATCGGA909580.2675441853966667No Hit
AGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC864080.2541607991793484No Hit
TTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGGA835670.24580427165332616No Hit
CTGTAGGCACCATCAATAGACCGGAAGAGCACACGTCTGAACTCCAGTCA810110.238286044143114Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
TTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT793690.23345625949062243No Hit
ATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGA758380.22307016350527065No Hit
TCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGAAGAGCACA750400.22072292346100253No Hit
TCCCACGGCGCGACCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCA743890.21880806974067848No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCACTATCAATAGATCGGA742940.21852863640207518No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGCATCATCAATAGATCGGA739240.21744031708330422No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC737200.2168402707561981Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT728760.21435772614797466Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACAC689690.20286566242246643No Hit
CTGTAGGCACCATCAATAGAACGGAAGAGCACACGTCTGAACTCCAGTCA657500.19339728434915932Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
CCGCCACGCAGTTTTATCCGGTAACTGTAGGCACCATCAATAGATCGGAA652220.19184422326723755No Hit
CTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGA622830.18319943819191922No Hit
ACGTCTGCCCTATCAACTTCTGTAGGCACCATCAATAGATCGGAAGAGCA592980.1744193485526456No Hit
ATCATCGACACTTCGAACGCACTGTAGGCACCATCAATAGATCGGAAGAG584840.17202504605134955No Hit
AGCTAGGAATAATGGAATACTGTAGGCACCATCAATAGATCGGAAGAGCA583650.1716750190272043No Hit
TCATCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGGAAGAG581550.1710573242787127No Hit
TACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGA569720.16757764386220994No Hit
ACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAG559200.1644832873126234No Hit
CTGTAGGCACCATCAATAGATTGGAAGAGCACACGTCTGAACTCCAGTCA556120.1635773350148357Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCAGCATCT549910.1617507234014391TruSeq Adapter, Index 11 (97% over 36bp)
TCGACACTTCGAACGCACCTGTAGGCACCATCAATAGATCGGAAGAGCAC536290.15774453174693637No Hit
ACGACGCTCTTCCGATCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC525480.15456487449398668No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG511120.15034101897572974No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCG510910.1502792495008806No Hit
ATGCGTGCATTTATCAGACTGTAGGCACCATCAATAGATCGGAAGAGCAC510750.15023218704385263No Hit
ATAACCTCGGGCCGATCGCACTGTAGGCACCATCAATAGATCGGAAGAGC504040.14825850525224374No Hit
AGCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAATAGATCGGA479710.14110207038043376No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCAA473760.13935193525970754No Hit
ATTGATCATCGACACTTCGAACGCACTGTAGGTACCATCAATAGATCGGA466440.13719882785067963No Hit
ATTGATCATCGACACTTCGATCGCACTGTAGGCACCATCAATAGATCGGA455910.13410152989752885No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCCCTGTAGGCACCATCAATAGATCG453190.133301468128054No Hit
TCCTTCTGATCGAGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA440730.12963647928700378No Hit
GCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATAGATCGGAAGAGCA430260.12655682975523846No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATAGATC428670.12608914658852338No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA422700.12433312866066866No Hit
GCAGCTAGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGC422280.12420958971097033No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCCTGTAGGCACCATCAATAGATCGG414730.12198883001996479No Hit
ACCTCGGGCCGATCGCACTGTAGGCACCATCAATAGATCGGAAGAGCACA390050.1147294460234062No Hit
AAGTCCTTCTGATCGAGGCCTGTAGGCACCATCAATAGATCGGAAGAGCA387710.11404115758937271No Hit
ATGTTGGAACAATGTAGGTAAGGGACTGTAGGCACCATCAATAGATCGGA387190.11388820460403193No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGTAGGCACCATCAA383160.1127028189676409No Hit
GCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC379830.11172333158074704No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTCTGTAGGCACCATCAAT374130.11004673154912696No Hit
CCGCCACGCAGTTTTATCCGGTAAACTGTAGGCACCATCAATAGATCGGA368340.10834365888542866No Hit
TCCCACGGCGCGACCGCTCTCTGTAGGCACCATCAATAGATCGGAAGAGC368050.10825835818206553No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)