FastQCFastQC Report
Tue 19 Sept 2023
SRR16301570_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16301570_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72114788
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT20057912.781386530596193No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT10062171.395299116735946No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT5021960.696384214566366No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATATC4488780.6224493095646346No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC3142440.4357552850325235No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATATC2977300.4128556822492496No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG2742050.3802340790352181No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTA2710780.3758979364953552No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT2526430.3503345250075477No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC2463120.3415554657111382No Hit
TTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCTC2106110.29204966947971894No Hit
GCCCGGATAGCTCAGTCGGTAGAGCAGGGGCTGTAGGCACCATCAATATC2041840.28313748908198966No Hit
ATCACGGGAGACACACGGCGGGTGCTGTAGGCACCATCAATATCTCGTAT1961410.2719844368120447No Hit
ACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATATCTCG1956340.2712813909956998No Hit
TAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCT1747340.2422998178958801No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG1733720.24041116227090623No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATAT1687290.23397281567270226No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA1599730.22183106188983043No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATC1597270.22148993906769857No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT1572220.21801631033013646No Hit
GGCTACGTAGCTCAGTTGGTTAGAGCACATCCTGTAGGCACCATCAATAT1381080.19151134438611953No Hit
TGCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATAT1329800.18440045889062312No Hit
CAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT1327930.18414115007867735No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1217370.1688100365766866Illumina PCR Primer Index 6 (96% over 29bp)
TTGAGGCGTGGCTTCCGGAGCTAACGCGTTCTGTAGGCACCATCAATATC1213450.16826645874629764No Hit
TGCCCGGATAGCTCAGTCGGTAGAGCAGGGGCTGTAGGCACCATCAATAT1166600.1617698716662663No Hit
GGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT1131380.1568859912615981No Hit
GGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGTAT1104710.1531877206655589No Hit
TAGGCGTGTGACTGCGTACCTTTTGCTGTAGGCACCATCAATATCTCGTA1094160.15172477522918046No Hit
ACTAACTGGAGGACCGAACCGACCTGTAGGCACCATCAATATCTCGTATG1079320.14966694487127938No Hit
AGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATATCTCGTATGC1057040.14657742597815027No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCATCAATAT1021660.14167135872326214No Hit
TTTCCCTGGTTCGAATCCAGGTACCCCAGCCACTGTAGGCACCATCAATA1004480.1392890456808942No Hit
TTGGGGTATCGCCAAGCGGTAAGGCACCGGCTGTAGGCACCATCAATATC1003580.13916424464840693No Hit
TTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATATCT997490.1383197576619098No Hit
TGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT977930.13560741522251996No Hit
ACCTGCGGTTGGATCACCTCCCTGTAGGCACCATCAATATCTCGTATGCC957350.1327536316129779No Hit
TAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA952210.13204087904966177No Hit
TTGAGGCGAACCGGGGGAACTGCTGTAGGCACCATCAATATCTCGTATGC922110.12786697785203222No Hit
CGCTGTGCGGGTTCAAGTCCCGCTCCGGGCTGTAGGCACCATCAATATCT911160.12634856529010388No Hit
TTGAGGCGTGGCTTCCGGAGCTAACGCGTTACTGTAGGCACCATCAATAT904500.12542503764969815No Hit
TAGGCGTGTGACTGCGTACCTTTTGTACTGTAGGCACCATCAATATCTCG897910.12451121675626363No Hit
GAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT884680.1226766415787009No Hit
ACGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATATCTCGT861780.11950114864096946No Hit
AACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGT856480.11876620922743335No Hit
TACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATATCTC818710.11352872589738461No Hit
TGCCGACTTAGCTCAGTAGGTAGAGCAACTGTAGGCACCATCAATATCTC811330.11250535743098905No Hit
TAGGCGTGTGACTGCGTACCTTTTGTATCTGTAGGCACCATCAATATCTC807950.11203666022009244No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGTAGGCACCATCAATATCTCG799180.11082054349241101No Hit
TAGGCGTGTGACTGCGTACCTTTTGTATACTGTAGGCACCATCAATATCT780800.10827182907339337No Hit
GCCCGGATAGCTCAGTCGGTAGAGCAGGGGACTGTAGGCACCATCAATAT752780.10438635692862329No Hit
NAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT736490.10212745824060385No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)