FastQCFastQC Report
Tue 19 Sept 2023
SRR16301571_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16301571_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72893291
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT29541584.052715907695813No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT19024672.6099342942274344No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT6860870.9412210514682346No Hit
ACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATATCTCG6012590.8248482017364259No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT5340100.7325914260065443No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT4193530.575297114792087No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTA3960680.5433531598950581No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG3788060.5196719681650812No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATATC3546360.48651390976434306No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC3292270.4516561064584119No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATATC3043840.4175747806475084No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC2647530.3632062654435509No Hit
CAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT2561810.35144660981214304No Hit
AGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATATCTCGTATGC2475990.3396732354970775No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG2417820.3316930772133748No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA2348980.32224913538339217No Hit
TACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATATCTC2277080.31238540183348285No Hit
GGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGTAT2262570.31039482083474595No Hit
ACGCTTTAGCAGCTTAATAACCTGCTCTGTAGGCACCATCAATATCTCGT2209310.3030882499186379No Hit
TTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCTC2096910.2876684494873472No Hit
ATCACGGGAGACACACGGCGGGTGCTGTAGGCACCATCAATATCTCGTAT2046510.28075423292385027No Hit
CGCTGTGCGGGTTCAAGTCCCGCTCCGGGCTGTAGGCACCATCAATATCT1825670.25045789193411505No Hit
TTGAGGCGTGGCTTCCGGAGCTAACGCGTTCTGTAGGCACCATCAATATC1821300.24985838545827213No Hit
TAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA1759360.241361032800673No Hit
TAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCT1726460.23684758587727917No Hit
GGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT1634710.22426069362131007No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATAT1634470.22422776878053152No Hit
GAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT1631290.22379151464021566No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1588810.21796381782241112Illumina PCR Primer Index 6 (96% over 29bp)
ACTAACTGGAGGACCGAACCGACCTGTAGGCACCATCAATATCTCGTATG1571420.21557813873433151No Hit
TTATCCTGTCTGAATATGGGGGGACTGTAGGCACCATCAATATCTCGTAT1566890.21495668236463628No Hit
GGCTACGTAGCTCAGTTGGTTAGAGCACATCCTGTAGGCACCATCAATAT1506620.2066884317241212No Hit
TGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT1462240.20060007991681977No Hit
TAGGCGTGTGACTGCGTACCTTTTGCTGTAGGCACCATCAATATCTCGTA1359740.18653842916764451No Hit
AGCGGATGACTTGTGGCTGGGGGCTGTAGGCACCATCAATATCTCGTATG1266680.17377182215575915No Hit
TAGGCGTGTGACTGCGTACCTTTTGTCTGTAGGCACCATCAATATCTCGT1260760.17295967608322144No Hit
AACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGT1258970.17271411164574804No Hit
TAGGCGTGTGACTGCGTACCTTTTGTACTGTAGGCACCATCAATATCTCG1231730.16897714221738186No Hit
TGCGCTGTGCGGGTTCAAGTCCCGCTCCGGGCTGTAGGCACCATCAATAT1228650.1685546067607237No Hit
NAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT1194810.1639122042109472No Hit
AGCGGATGACTTGTGGCTGGGGCTGTAGGCACCATCAATATCTCGTATGC1164610.15976916174631214No Hit
ACGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATATCTCGT1149680.15772096227621277No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATC1104180.15147896121194473No Hit
TAGGCGTGTGACTGCGTACCTTTTGTATCTGTAGGCACCATCAATATCTC1085150.14886829571187835No Hit
TTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATATCT1075210.1475046585562998No Hit
GCCCGGATAGCTCAGTCGGTAGAGCAGGGGCTGTAGGCACCATCAATATC1031270.14147666895709235No Hit
TTGAGGCGTGGCTTCCGGAGCTAACGCGTTACTGTAGGCACCATCAATAT1019810.13990450780991628No Hit
TTGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATATCTCGTATG996220.1366682703350573No Hit
TAGGCGTGTGACTGCGTACCTTTTGTATACTGTAGGCACCATCAATATCT929920.12757278306998104No Hit
TAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA915720.12562472999058308No Hit
TTGAGGCGAACCGGGGGAACTGCTGTAGGCACCATCAATATCTCGTATGC915250.12556025217739175No Hit
TACGCTTTAGCAGCTTAATAACCTGCTCTGTAGGCACCATCAATATCTCG881080.12087257797154473No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCATCAATAT865040.11867210111284454No Hit
TTACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATATCT842270.11554835684397897No Hit
TTAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG833010.11427800673727297No Hit
AAAACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTC824040.11304744081317443No Hit
AAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTGTAGGCACCATCAATA823870.11302411905095629No Hit
CGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTAT822430.11282657000628493No Hit
TGCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATAT818490.11228605387017031No Hit
AACGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATATCTCG802020.11002658667174185No Hit
TGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCT773160.1060673745681204No Hit
ACTAACTGGAGGACCGAACCGACTCTGTAGGCACCATCAATATCTCGTAT767590.10530324388838475No Hit
TAAACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTC764250.10484503985421648No Hit
TTGGGGTATCGCCAAGCGGTAAGGCACCGGCTGTAGGCACCATCAATATC755430.10363505195560452No Hit
TTGAGGCGTGGCTTCCGGAGCTAACGCGTCTGTAGGCACCATCAATATCT741000.10165544590379381No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)