FastQCFastQC Report
Tue 19 Sept 2023
SRR16301572_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16301572_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70429335
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT48200966.843875495913741No Hit
TAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT32726634.646732785422438No Hit
ACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATATCTCG8321351.1815176162035324No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG5882930.835295406381446No Hit
AAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT5630950.7995177009693475No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTA5314970.7546528729825434No Hit
CAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT4373910.6210352546989121No Hit
AAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA4287460.6087605399085481No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATATC3885980.5517558841070982No Hit
TAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTAT3809130.5408442377029401No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG3786420.537619729051822No Hit
ACGCTTTAGCAGCTTAATAACCTGCTCTGTAGGCACCATCAATATCTCGT3347230.47526077024580743No Hit
GGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGTAT3300140.46857463583888737No Hit
TACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATATCTC3236570.45954856736898053No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCT2920580.4146823195192742No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC2897080.41134564169887444No Hit
GAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT2763990.39244868633219376No Hit
TAGGCTGAGGCGTGATGACGAGGCACTGTAGGCACCATCAATATCTCGTA2670790.37921556408277884No Hit
AGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCTC2659220.37757278270482036No Hit
ATCACGGGAGACACACGGCGGGTGCTGTAGGCACCATCAATATCTCGTAT2466550.3502162841662498No Hit
ACTAACTGGAGGACCGAACCGACCTGTAGGCACCATCAATATCTCGTATG2348470.33345054301591803No Hit
TTATCCTGTCTGAATATGGGGGGACTGTAGGCACCATCAATATCTCGTAT2258060.3206135625162441No Hit
GGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT2231440.31683388747032754No Hit
NAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT2016640.286335232328972No Hit
TGGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTAT1893480.268848200824273No Hit
TTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATATC1851550.26289471567493855No Hit
TAGGCGTGTGACTGCGTACCTTTTGCTGTAGGCACCATCAATATCTCGTA1818220.2581623126215802No Hit
TTGAGGCGTGGCTTCCGGAGCTAACGCGTTCTGTAGGCACCATCAATATC1806980.2565663867193975No Hit
GGCTACGTAGCTCAGTTGGTTAGAGCACATCCTGTAGGCACCATCAATAT1770760.2514236432872751No Hit
AGCGGATGACTTGTGGCTGGGGCTGTAGGCACCATCAATATCTCGTATGC1609910.22858514850381592No Hit
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1601560.2273995629803973Illumina PCR Primer Index 6 (96% over 29bp)
AGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGTA1568340.2226827784189642No Hit
TAGGCACGCTGATATGTAGGTGAAGCGACCTGTAGGCACCATCAATATCT1545750.2194753081226736No Hit
AGCGGATGACTTGTGGCTGGGGGCTGTAGGCACCATCAATATCTCGTATG1522310.2161471494796877No Hit
TTGAGGCGAACCGGGGGAACTGCTGTAGGCACCATCAATATCTCGTATGC1505290.21373054281997694No Hit
TAGGCGTGTGACTGCGTACCTTTTGTACTGTAGGCACCATCAATATCTCG1496460.21247680387724804No Hit
ACGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATATCTCGT1464480.20793608231569985No Hit
TTGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATATCTCGTATG1456940.20686550568736733No Hit
TTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATCTC1455160.20661277009075835No Hit
TACGCTTTAGCAGCTTAATAACCTGCTCTGTAGGCACCATCAATATCTCG1389200.19724735438720245No Hit
TTAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG1355000.19239142326134417No Hit
AACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGT1304380.185204077249913No Hit
CGCTGTGCGGGTTCAAGTCCCGCTCCGGGCTGTAGGCACCATCAATATCT1290830.18328016301729955No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATC1272170.18063069884161195No Hit
TTGAGGCGTGGCTTCCGGAGCTAACGCGTCTGTAGGCACCATCAATATCT1177100.1671320622294673No Hit
GCCCGGATAGCTCAGTCGGTAGAGCAGGGGCTGTAGGCACCATCAATATC1159150.16458340831984286No Hit
TGTACGTACAAGCAGTGGGAGCACGCCTGTAGGCACCATCAATATCTCGT1156380.16419010629590639No Hit
TAGGCGTGTGACTGCGTACCTTTTGTCTGTAGGCACCATCAATATCTCGT1107980.15731796984878532No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATATC1084020.15391597833487994No Hit
TGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCAATATCT1034920.146944451484598No Hit
CGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTAT1022160.14513270642126608No Hit
AGTTCAGTCGGTAGAACGGCGGCTGTAGGCACCATCAATATCTCGTATGC1019830.14480187836503072No Hit
GGTTAAGCGACTAAGCGTACACGGTCTGTAGGCACCATCAATATCTCGTA1018310.14458605920388146No Hit
TAGGCGTGTGACTGCGTACCTTTTGTATCTGTAGGCACCATCAATATCTC1001600.14221346829414191No Hit
TAGGCGTGTGACTGCGTACCTTTTGTATACTGTAGGCACCATCAATATCT992180.14087595744017745No Hit
TTACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATATCT992110.14086601839986135No Hit
GGGGCTGATTCTGGATTCGACGGCTGTAGGCACCATCAATATCTCGTATG960410.13636505299957752No Hit
TGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGTA948190.13462998053296968No Hit
TTGAGGCGTGGCTTCCGGAGCTAACGCGTTACTGTAGGCACCATCAATAT910560.1292870364316233No Hit
TTGTCCCGGTTTAAGCGTGTAGGCTGCTGTAGGCACCATCAATATCTCGT910060.1292160432865084No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACTCCCTGTAGGCACCATCAATAT907950.12891645221412357No Hit
ACTAATGAACCGTGAGGCTTAACCCTGTAGGCACCATCAATATCTCGTAT877980.12466112309593722No Hit
ATGAGGCGAACCGGGGGAACTGCTGTAGGCACCATCAATATCTCGTATGC874210.12412583478177099No Hit
GGTACTAATGAACCGTGAGGCTTAACCCTGTAGGCACCATCAATATCTCG866920.12309075472599591No Hit
ACCTGCGGTTGGATCACCTCCCTGTAGGCACCATCAATATCTCGTATGCC862270.12243051847642748No Hit
TAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTGTAGGCACCATCAATAT858790.12193640618642786No Hit
TTGGGGTATCGCCAAGCGGTAAGGCACCGGCTGTAGGCACCATCAATATC838490.11905408449476343No Hit
TGCCCTGCTTCCAGGAAAAGCCTCTAAGCCTGTAGGCACCATCAATATCT833730.11837822975326973No Hit
AAATTGAAGAGTTTGATCATGGCTCACTGTAGGCACCATCAATATCTCGT822210.11674254768982273No Hit
TGCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATAT819020.11628961142398973No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGTAGGCACCATCAATATCTCG817680.11609934979508182No Hit
CGCAGGTTCGAATCCTGCACGACCCCTGTAGGCACCATCAATATCTCGTA815740.11582389639203607No Hit
TGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATATCTCGTA802200.11390140202232493No Hit
AAATTGAAGAGTTTGATCATGGCTCCTGTAGGCACCATCAATATCTCGTA801790.11384318764333073No Hit
ACTAACTGGAGGACCGAACCGACTCTGTAGGCACCATCAATATCTCGTAT797550.11324116577275647No Hit
AACGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATATCTCG794120.11275415279726834No Hit
ATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATATCTCGTATG783270.1112136015482753No Hit
TGAAAGCTATTTAGGTAGCGCCTCGTGACTGTAGGCACCATCAATATCTC775260.1100762913635348No Hit
ATCAGGTAACATCAAATCGTACCCCCTGTAGGCACCATCAATATCTCGTA767520.10897731747715636No Hit
TATGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGTATG766120.10877853667083467No Hit
ATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTATGC756530.10741688814753114No Hit
GGTTAAGCGACTAAGCGTACACGGTGCTGTAGGCACCATCAATATCTCGT754880.10718261076865201No Hit
GGGGGCTGATTCTGGATTCGACGGGCTGTAGGCACCATCAATATCTCGTA731040.1037976576095742No Hit
CGGTACTAATGAACCGTGAGGCTTAACCCTGTAGGCACCATCAATATCTC729660.10360171652905711No Hit
TAAGGCTGAGGCGTGATGACGAGGCCTGTAGGCACCATCAATATCTCGTA717190.10183114748989182No Hit
AAATTGAAGAGTTTGATCATGGCTCTGTAGGCACCATCAATATCTCGTAT713180.10126178246607043No Hit
GGGTCGTTAGCTCAGTTGGTAGAGCCTGTAGGCACCATCAATATCTCGTA707330.10043116266822624No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)