FastQCFastQC Report
Fri 29 Sept 2023
SRR16312993.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR16312993.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41940755
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA487723711.628872680045935No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA37758809.002889909826372No Hit
CTCGGTTGGCCTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAACT10893632.5973852878900248RNA PCR Primer, Index 1 (100% over 25bp)
CTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAACT10569702.5201501498959664RNA PCR Primer, Index 1 (100% over 25bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAAC9106462.1712675415595166RNA PCR Primer, Index 1 (100% over 24bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA8410312.0052834051270656No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGT8036281.9161028455496332No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG5299791.2636372425818276No Hit
CTAAACCATTCGTAGACGACCTGCTTTGGAATTCTCGGGTGCCAAGGAAC4642071.1068160313280007RNA PCR Primer, Index 1 (100% over 24bp)
CGTTTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGG4474111.0667690650776316Illumina Small RNA Adapter 2 (100% over 21bp)
TCGGTTGGCCTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAACTC4120700.9825049644432963RNA PCR Primer, Index 1 (100% over 26bp)
GCCTCGGTTGGCCTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAA4055200.9668876967045539RNA PCR Primer, Index 1 (100% over 23bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCC3875620.9240701556278612No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC3622240.8636563647936237No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA3518170.8388427914566631No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG3356280.8002431048272736No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAACT3318240.7911731679603765RNA PCR Primer, Index 1 (100% over 25bp)
CCTCGGTTGGCCTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAAC3249010.7746665504710156RNA PCR Primer, Index 1 (100% over 24bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG3224810.7688965065125795No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCCAAGGAA2945360.702266804686754RNA PCR Primer, Index 1 (100% over 23bp)
GCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAGG2895530.6903857596268832Illumina Small RNA Adapter 2 (100% over 21bp)
TCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAAGGAACTC2791610.6656079510252021RNA PCR Primer, Index 1 (100% over 26bp)
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATC2747890.6551837228490522RNA PCR Primer, Index 2 (100% over 42bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC2657700.6336795796832937No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGG2477770.5907785875576155Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG2021810.48206332957048575No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTGC1932490.46076662186934875No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC1912800.45607190428498484No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGTGGAATTCTCGGGTGCCAA1801360.42950109028795497No Hit
CGGTTGGCCTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGAACTCC1724910.4112729968738045RNA PCR Primer, Index 1 (100% over 27bp)
CTCGCTTCTGGCGCCAAGCGCCCGTGGAATTCTCGGGTGCCAAGGAACTC1648130.3929662210420389RNA PCR Primer, Index 1 (100% over 26bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCT1631040.3888914255358541RNA PCR Primer, Index 2 (100% over 43bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTGGAATTCTCGGGTGCCAAG1630190.3886887587026032No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTAGTGGAATTCTCGGGTGCCA1458940.34785735259176903No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGA1414270.33720661442551525RNA PCR Primer, Index 1 (100% over 22bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTGCCAAGGA1388900.3311576055318985RNA PCR Primer, Index 1 (100% over 22bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTATGGAATTCTCGGGTGCCAAG1298490.30960100742106333No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG1238290.29524742699553214No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGGA1229420.29313253898266733RNA PCR Primer, Index 1 (100% over 22bp)
CGCTGAGAAGACGGTCGAACTTGGAATTCTCGGGTGCCAAGGAACTCCAG1089050.2596638997080525RNA PCR Primer, Index 1 (100% over 29bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC1013900.2417457673329915No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC988570.235706295702116No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCCAAGGAAC948990.22626917421968204RNA PCR Primer, Index 1 (100% over 24bp)
TTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAAC947950.2260212053884104RNA PCR Primer, Index 1 (100% over 24bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAGG826150.1969802403414054Illumina Small RNA Adapter 2 (100% over 21bp)
GGATGGTTTAGTGAGGCCCTCGGATCGGCCTGGAATTCTCGGGTGCCAAG797570.19016586611280603No Hit
AGGTCGGTTTCTATCTACTTCAAATTCCTTGGAATTCTCGGGTGCCAAGG776960.1852517914853941Illumina Small RNA Adapter 2 (100% over 21bp)
CGCTGAGAAGACGGTCGAACTTGTGGAATTCTCGGGTGCCAAGGAACTCC766570.18277448748836306RNA PCR Primer, Index 1 (100% over 27bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA744810.17758621655714113No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCA742570.177052129843633No Hit
TTTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAGGAA739020.17620569777535003RNA PCR Primer, Index 1 (100% over 23bp)
CCTCGGTTGGCCTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGG733480.1748847868856915Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG717420.171055575895093No Hit
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC678250.16171621135575648RNA PCR Primer, Index 1 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA673130.16049544172488073No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA662760.15802290635922028No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGT573710.1367905751815865No Hit
GCTGGTCCGAAGGTAGTGAGTTATCTCAATGGAATTCTCGGGTGCCAAGG526700.1255819071449715Illumina Small RNA Adapter 2 (100% over 21bp)
TCGGTTGGCCTCGGATAGCCGGTCCCCTGGAATTCTCGGGTGCCAAGGAA514320.12263012432656493RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT511890.12205073561503602No Hit
CTAAACCATTCGTAGACGACCTGCTTCTGGAATTCTCGGGTGCCAAGGAA505050.12041986368628796RNA PCR Primer, Index 1 (100% over 23bp)
TCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTGCCAAGGAA501370.119542435514096RNA PCR Primer, Index 1 (100% over 23bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCA492780.11749430834041971No Hit
CTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAGGA491060.11708420604254739RNA PCR Primer, Index 1 (100% over 22bp)
AGCCTCGGTTGGCCTCGGATAGCCGGTCTGGAATTCTCGGGTGCCAAGGA478370.11405850943789637RNA PCR Primer, Index 1 (100% over 22bp)
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGGAATTCTCGGGTGC478130.11400128586144909No Hit
AACCTCGGGCCGATCGCACTGGAATTCTCGGGTGCCAAGGAACTCCAGTC474860.11322161463235461RNA PCR Primer, Index 1 (100% over 31bp)
GCTGAGAAGACGGTCGAACTTGACTATCTAGTGGAATTCTCGGGTGCCAA473300.11284966138544716No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA466730.1112831659802023No Hit
CGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAACTC456740.10890123461058342RNA PCR Primer, Index 1 (100% over 26bp)
CTCGGTTGGCCTCGGATAGCCGGTCCCTGGAATTCTCGGGTGCCAAGGAA432940.10322656327955947RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)